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EC number: 458-890-5 | CAS number: 2138848-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 458-890-5
- EC Name:
- -
- Cas Number:
- 2138848-94-7
- Molecular formula:
- C35H31N8O18S3.xNa.yK, where x+y=3
- IUPAC Name:
- dipotassium sodium 3-[(1E)-2-{4-[(1E)-2-{4-[(1E)-2-(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl)diazen-1-yl]-2-sulfonatophenyl}diazen-1-yl]-2,5-bis(2-hydroxyethoxy)phenyl}diazen-1-yl]-4,5-dihydroxynaphthalene-2,7-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- S191820 concentration measured at 0 and 7 days
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Culture medium control and nominal concentrations 0f 5.0, 11, 25, 55 and 120 mg/l
Test organisms
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- The test organism was the duckweed Lemna minor Strain ST from laboratory cultures maintained under axenic conditions. The cultures were originally obtained from the Botanical Institute of the University of Jena, Germany.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Hardness:
- Not Applicable
- Test temperature:
- 24 degrees centigrade +/- 2 degrees
- pH:
- At the start of the test the pH ranged from 5.6 to 5.7 and at the end of the test the range was from 6.2 to 6.3.
- Dissolved oxygen:
- Not Applicable
- Salinity:
- Not Applicable
- Nominal and measured concentrations:
- Test concentrations were culture medium control and S191820 at nominal concnetrations of 5.0, 11, 25, 55 and 120 mg/l. The mean measured concentrations ranged from90 to 100% of the nominal values.
- Details on test conditions:
- The test vessels were borosilicate glass cylindrical dishes of 300 ml nominal capacity with loose-fitting lids. Each vessel contained 160 ml of test solution. The cultures were incubated at 24 ± 2C (the nominal test temperature), under continuous illumination, of approximately 8200 lux, using "warm-white" lights in an incubator.
- Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (frond number)
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (frond number)
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (frond number)
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry weight
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry weight
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry weight
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (dry weight)
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (dry weight)
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Specific growth rate (dry weight)
- Details on results:
- Individual results as follows:
Based on the increase in number of fronds the results obtained were:
No observed effect (P=0.05) concentrations (NOEC) = 25 mg l-1
Lowest observed effect (P=0.05) concentration (LOEC) = 55 mg l-1
Median effective concentration, EyC50 (frond number) >120 mg l-1
Based on average specific growth rate (frond number) the results obtained were:
No observed effect (P=0.05) concentrations (NOEC) = 25 mg l-1
Lowest observed effect (P=0.05) concentration (LOEC) = 55 mg l-1
Median effective concentration, ErC50 (frond number) >120 mg l-1
Based on the increase in dry weight of the plants over the test period, the results obtained were:
No observed effect (P=0.05) concentration (NOEC) = 25 mg l-1
Lowest observed effect (P=0.05) concentration (LOEC) = 55 mg l-1
Median effective concentration, EyC50 (dry weight) >120 mg l-1
Based on average specific growth rate (dry weight) the results obtained were:
No observed effect (P=0.05) concentrations (NOEC) = 25 mg l-1
Lowest observed effect (P=0.05) concentration (LOEC) = 55 mg l-1
Median effective concentration, ErC50 (dry weight) >120 mg l-1 - Reported statistics and error estimates:
- The number of fronds and numbers of plants in each test vessel was measured on days 2,5 and 7.
The data for increase in frond number, growth rate and dry weight were examined by one-way analysis of variance, and a multiple comparison procedure (Dunnett’s t-test) was used to identify significant differences (P=0.05, two tailed) from the culture medium control.
No attempt was made to analyse the data for plant numbers, since frond numbers and weight increases were considered more reliable estimates of Lemna growth.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- S191820 is considered to be non-toxic to duckweed (lemna minor sp.)
- Executive summary:
S191820 is considered to be non-toxic to duckweed (Lemna minor sp.)
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