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EC number: 305-673-7 | CAS number: 94944-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 January 2018 to 18 January 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no analytics were performed because it was not possible to develop a suitable analytical method
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- it was not possible to develop a suitable analytical method for this complex UVCB
- Vehicle:
- no
- Details on test solutions:
- The 100 mg/L WAF was prepared by adding a nominal 0.2 g of test substance (actual weight: 0.20008 g) to 2000 mL of test media. The control was treated the same without the addition of test substance.
The solutions were stirred for 24 hours and left to settle for approximately 1 hour 15 mins. Undissolved test substance was observed on the surface of the solution, on the sides and bottom of the vessel, in the outlet tube and a large amount was observed in the protruding vessel tap area. Fine particles of test substance were visible throughout the solution. The first ~100 mL drawn off the vessel went to waste to clear the outlet, followed by the water-soluble portion from the middle of the vessel. The solutions were higher than the test temperature due to stirring, so were cooled using an ice bath to a nominal temperature of 20°C. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphmia magna
- Source: laboratory cultures held at Scymaris.
- Age of parental stock (mean and range, SD): 22 ± 1 days (daphnids in test < 24 h)
- Feeding during test: none
ACCLIMATION
- Stock conditions (same as test or not): yes
- Type and amount of food: mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4
- Feeding frequency: daily ad libitum
- Health during acclimation: no evidence of diease before test:
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 248.7 as CaCO2
- Test temperature:
- 18.7-20.6 ˚C
- pH:
- 7.64 - 7.94
- Dissolved oxygen:
- 8.96-9.08 mg/L
- Nominal and measured concentrations:
- nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers of 250 mL
- Type (delete if not applicable):closed
- Fill volume: 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS: The reconstituted water medium used for testing (and maintenance of stock cultures) was Elendt's M4 D. magna medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8
EFFECT PARAMETERS MEASURED : immobilisation at 24 and 48 h
VEHICLE CONTROL PERFORMED: NA
RANGE-FINDING STUDY: not performed, 100 mg/L WAFwas prepared - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Based on immobility compared to the control (from direct observation of the data), the 48 hour No Observed Effect Concentration (NOEC) was determined to be 100 mg/L and the Lowest Observed Effect Concentration (LOEC) was >100 mg/L.
There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed. - Reported statistics and error estimates:
- Linear interpolation (ICPIN)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for Daphnia magna is > 100 mg/L (nominal)
- Executive summary:
Daphnids were exposed during 48 h (static test) to control and 100 mg/L (WAF).
Based on immobility compared to the control (from direct observation of the data), the 48 hour No Observed Effect Concentration (NOEC) was determined to be 100 mg/L. The EC50 is > 100 mg/L
There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed.
Reference
Daphnia magna response
Time (hour) |
Nominal concentration of the substance (mg/L) |
Number immobilised per replicate |
Total number tested |
Total number immobilised |
Percentage immobilised |
|||
A |
B |
C |
D |
|||||
24 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
48 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
Description of key information
Daphnids were exposed during 48 h (static test) to control and 100 mg/L (WAF)
Based on immobility compared to the control (from direct observation of the data), the 48 hour NOELR was determined to be 100 mg/L. The EL50 is > 100 mg/L.
There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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