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EC number: 275-912-7 | CAS number: 71720-84-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted ApriI 4, 1984
- GLP compliance:
- no
- Details on test solutions:
- The test article was dissolved in bidistilled water at a concentration of 500 mg/l.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: the tests were run with aerobic activated sludge from a domestic waste-water treatment plant (ARA, Sissach/Switzerland).
- Pretreatment: the sludge was coarsely sieved, centrifuged, the supernatant liquid phase decanted, the solid material resuspended in tap water and again centrifuged. This procedure was repeated a further two times.
- Initial biomass concentration: a volume of washed sludge suspension corresponding to 4.0 g dry material were made up with Soerensen buffer (pH = 7) to 1 litre.
- Preparation of inoculum for exposure: 50 ml OECD recommended synthetic sewage feed were added to sludge suspension.
- Incubation: the sludge was incubated overnight at 20 - 25 °C under aeration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- pH:
- 7.8 at the start
- Nominal and measured concentrations:
- 10 and 100 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flasks (> 500 ml).
- Aeration: 0.2 l/minute instead of 0.5 l/minute to prevent possible foam development.
- No. of vessels per concentration: one replicate.
- No. of vessels per control: 2 replicates.
- Sludge amount: 200 ml
SAMPLING AND ANALYSIS
After 180 minutes of aeration at 22.0 °C, the first sample was taken for oxygen measurement over a period of up to 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration.
Sampling was started with the control 1. Thereafter, the measurements were continued with the positive controls and the test article samples. Finally, the oxygen consumption of control 2 was determined.
MEASUREMENTS
Oxygen consumption was measured over a period of 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
VALIDITY OF THE TEST
The tests are validated by testing a known standard, i.e. 3,5-dichlorophenol. The IC50-value should be in the range of 5 - 30 mg/l. Furthermore, the variation between the two control samples (start, end) should not be greater than 15 %. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, 3.2, 10 and 50 mg/l
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The test article showed only a slight inhibition (17.0 %) of the respiration rate up to a concentration of 100 mg/l.
The aeration of the test medium with test article caused the solutions to foam strongly. - Results with reference substance (positive control):
- At a concentration of the standard of 10 mg/l, the respiration rate was highly inhibited by 75.5 %. The IC50 was found to be about 9.0 mg/l. Almost complete inhibition (91.5 %) was observed at 50 mg/l.
- Validity criteria fulfilled:
- yes
- Remarks:
- the IC50-value of reference substance was in the range of 5 - 30 mg/l and the variation between the two control samples (start, end) was not greater than 15 %
- Conclusions:
- IC50 (3h) > 100 mg/l (nominal)
- Executive summary:
The inhibitory effect of test item on aerobic waste-water bacteria was investigated in a respiration test, according to the OECD guideline 209. The method determines the respiration rate (oxygen consumption) of an aerobic activated sludge fed with an excess but standard amount of biodegradable food and as compared to the respiration rate obtained in an activated sludge run under identica conditions but containing the test chemical.
The test article showed only a slight inhibition (17.0 %) of the respiration rate up to a concentration of 100 mg/l. The IC50 was determined to be higher than 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l (nominal)
Reference
Influence of test item and reference substance on oxygen consumption of activated sludge.
Test substance (mg/l) | Oxygen consumption (mgO2/l min) | Inhibition (%) | Starting pH |
Control | 0.88 | - | 7.8 |
Control | 1.00 | - | 7.8 |
Mean |
0.94± 0.06 (13.6 %) | ||
Test item 100 | 0.78 | 17.0 | 7.8 |
Test item 10 | 0.91 | 3.2 | 7.8 |
3 ,5-dichlorophenol 50 | 0.08 | 91.5 | 7.8 |
3 ,5-dichlorophenol 10 | 0.23 | 75.5 | 7.8 |
3 ,5-dichlorophenol 3.2 | 0.88 | 6.4 | 7.8 |
Description of key information
IC50 (3h) > 100 mg/l (nominal)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
The inhibitory effect of test item on aerobic waste-water bacteria was investigated in a respiration test, according to the OECD guideline 209. The test article showed only a slight inhibition (17.0 %) of the respiration rate up to a concentration of 100 mg/l. The IC50 was determined to be higher than 100 mg/l.
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