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EC number: 274-668-9 | CAS number: 70546-25-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- The test item and reference compound concentrations were not confirmed by analytical methods, they were based on nominal concentrations.
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- incubation time with permanent aeration
- Test temperature:
- 20 +/- 2°C
- pH:
- pH of the suspension before application : 7.7
pH of the synthetic sewage feed : 7.5 ± 0.5
pH ranges after 3 hours incubation periods: 7.2 - 8.3 - Salinity:
- A synthetic waste water feed was prepared by dissolving the following amounts of substance per 1 litre of water.
16.0 g peptone
11.0 g meat extract
3.0 g urea
0.7 g NaCl
0.4 g CaCl2 x 2H2O
0.2 g MgSO4 x 7H2O
2.8 g K2HPO4 - Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- Test item concentration/s : 100 mg/L, 3 replicates
100 mg/L with ATU, 2 replicates
Control : 6 replicates
4 replicates with ATU
Test item concentration in physico-chemical oxygen consumption control : 100 mg/L
Concentration of reference compound 3,5-Dichlorophenol : 2.5, 5, 10, 20 and 40 mg/L
The test item and reference compound concentrations were not confirmed by analytical methods, they were based on nominal concentrations.
Test vessels : 300 mL glass Erlenmeyer flasks
Method of application : direct weighing
Test concentration of the activated sludge : 1200 mg/L suspended solids
Test temperature : 20 ± 2°C
Stirring period of the test item before start of incubation time : 17 hours
Incubation time : 3 hours with permanent aeration - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (Acros Organics, purity: 99.9%, Batch No.: A0357150))
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Macrolex Fluoreszenzrot G showed 1.5 % respiration inhibition of activated sludge at the highest test item concentration of 100 mg/L. The results is the eman ouot of three measurements. Macrolex Fluoreszenzrot G showed no statistical significant difference of respiration inhibition of activated sludge between control and a limit test item concentration of 100 mg/L.
- Results with reference substance (positive control):
- Concentration of the reference compound 3,5-Dichlorophenol was 2.5, 5, 10, 20 and 40 mg/L. The reference compound showed 15.76, 23.84, 45.90, 76.32 and 83.87 % inhibition, respectively.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Macrolex Fluoreszenzrot G showed no significant inhibitory effect at a limit test concentration of 100 mg/L (nominal).
- Executive summary:
The toxicity of Macrolex Fluoreszenzrot G was invetsigated according to OECD Guideline 209 and the EU method C.11. In order to do so, the activated sludge was exposed to Macrolex Fluoreszenzrot G at a limit test item concentration of 100 mg/L. The respiration rate of each mixture was determined after aeration periods of 3 hours. Macrolex Fluoreszenzrot G showed 1.5 % respiration inhibition of activated sludge at a test item concentration of 100 mg/L (nominal).
Based on this observation, the EC50 is determined to be higher than 100 mg/L (nominal) and the NOEC is equal or higher than 100 mg/L (nominal).
Reference
No inhibition was observed within the six replcated of the control.
Description of key information
Macrolex Fluoreszenzrot G showed no significant inhibitory effect at a limit test concentration of 100 mg/L (nominal).
Key value for chemical safety assessment
Additional information
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