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EC number: 251-974-0 | CAS number: 34375-28-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- The supporting QMRF report has been attached
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Prediction is done using OECD QSAR Toolbox version 3.3 with logKow as the primary descriptor.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2-(hydroxymethylamino)ethanol
- Molecular formula (if other than submission substance): C3H9NO2
- Molecular weight (if other than submission substance): 91.1091 g/mol
- Smiles notation (if other than submission substance): CN(CCO)O
- InChl (if other than submission substance): 1S/C3H9NO2/c1-4(6)2-3-5/h5-6H,2-3H2,1H3
- Substance type: Organic
- Physical state: Liquid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- 20 °C
- pH:
- 8.4 - 10.1
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 385.589 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The median Effective concentration (EC50) value for 2-[hydroxy(methyl)amino]ethanol on Desmodesmus subspicatus in a 72 hour study was estimated to be 385.6 mg/L on the basis of effects on growth rate.
- Executive summary:
Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 2-[hydroxy(methyl)amino]ethanol (CAS no. 34375 -28 -5). EC50 value was estimated to be 385.6 mg/l for Desmodesmus subspicatus for 72 h duration. Based on this value it can be concluded that the substance 2-[hydroxy(methyl)amino]ethanol is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Reference
The
prediction was based on dataset comprised from the following
descriptors: EC50
Estimation method: Takes average value from the 7 nearest neighbours
Domain logical expression:Result: In Domain
((((((("a"
or "b" or "c" or "d" )
and "e" )
and ("f"
and (
not "g")
)
)
and "h" )
and "i" )
and "j" )
and ("k"
and "l" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Alcohol OR N-Hydroxylamine
derivatives by Organic Functional groups ONLY
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Alcohol OR N-Hydroxylamine
derivatives OR Overlapping groups by Organic Functional groups (nested)
ONLY
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as Aliphatic Carbon [CH] OR
Aliphatic Carbon [-CH2-] OR Aliphatic Carbon [-CH3] OR Amino, aliphatic
attach [-N<] OR Hydroxy, aliphatic attach [-OH] OR Hydroxy, nitrogen
attach [-OH] OR Oxygen, nitrogen attach [-O-] by Organic functional
groups (US EPA) ONLY
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Alcohol OR Hydroxy compound OR
Hydroxylamine OR Primary alcohol by Organic functional groups, Norbert
Haider (checkmol) ONLY
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as No alert found by DNA binding by
OECD ONLY
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Non binder, non cyclic structure
by Estrogen Receptor Binding
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as Non binder, without OH or NH2
group OR Weak binder, OH group by Estrogen Receptor Binding
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Very fast by Bioaccumulation -
metabolism half-lives ONLY
Domain
logical expression index: "i"
Similarity
boundary:Target:
CN(O)CCO
Threshold=30%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization
Domain
logical expression index: "j"
Similarity
boundary:Target:
CN(O)CCO
Threshold=40%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization
Domain
logical expression index: "k"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= -3.86
Domain
logical expression index: "l"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 0.945
Description of key information
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 2-[hydroxy(methyl)amino]ethanol (CAS no. 34375 -28 -5). EC50 value was estimated to be 385.6 mg/l for Desmodesmus subspicatus for 72 h duration. Based on this value it can be concluded that the substance 2-[hydroxy(methyl)amino]ethanol is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 385.6 mg/L
Additional information
Various predicted data for the target chemical 2-[hydroxy(methyl)amino]ethanol(CAS No. 34375-28-5) and weight of evidence study for its read across substancewere reviewed to summarize the following information:
Short term toxicity on aquatic algae and cyanobacteria of target chemical2-[hydroxy(methyl)amino]ethanol(CAS No. 34375-28-5) is predicted using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances (2017). EC50 value was estimated to be 385.6 mg/l for Desmodesmus subspicatus for 72 h duration. Based on this value it can be concluded that the substance 2-[hydroxy(methyl)amino]ethanol is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
In another prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2017), the short term toxicity on green algae was predicted for test substance2-[hydroxy(methyl)amino]ethanol(CAS No. 34375-28-5).On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 25761.029 mg/l for green algae for 96 h duration. Based on this value, it can be concluded that the test chemical 2-[hydroxy (methyl)amino]ethanol can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classifiedas per the CLP classification criteria.
In a weight of evidencestudy from secondary source (OECD SIDS, 2006) of the read across chemical C.I. Pigment Black 7 (CAS no. 1333 -86 -4),short term toxicity to Selenastrum capricornutum (Green algae) study was carried out for 24 hrs. The study was performed according to ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum) and NF EN 28692 Guideline. The study was based on the effects of the read across compound C.I. Pigment Black 7 on Selenastrum capricornutum (green algae) in a static fresh water system. Selenastrum capricornutum was used as a test organism. After 72 hours of exposure to C.I. Pigment Black 7, the 72 hr EC50 value was determined to be > 13,000 mg/l. Thus, based on the EC50 value, it can be concluded that the substance C.I. Pigment Black 7 can be considered as non-toxic to Selenastrum capricornutum at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.
For the same read across chemical C.I. Pigment Black 7 (CAS no. 1333 -86 -4), short term toxicity to Scenedesmus subspicatus (Green algae) study was carried out for 72 hrs. The study was performed according to OECD Guide-line 201 "Algae, Growth Inhibition Test". The study was based on the effects of the read across compound C.I. Pigment Black 7 (CAS no. 1333 -86 -4) on Scenedesmus subspicatus (green algae) in a static fresh water system at a temperature range of 21.4 – 24.4°C, pH 8.15 and light intensity of greater than 120 µE/m2s, respectively. Scenedesmus subspicatus (at an initial cell density of 8 × 104cells/ml) was used as a test organism. The algal stock culture was cultivated under strictly aseptic conditions within Institut Fresenius. Test chemical suspensions was prepared in ultrapure water at 6250, 630, and 63 mg per 500 ml batch. After 24 hours incubation at room temperature on a shaking machine, during which time equilibration was considered to be achieved, the suspensions were filtered through a filter paper, which had previously been thoroughly rinsed with ultrapure water. These filtered solutions were then passed through sterile membrane filters to ensure sterile conditions for the test. Test chemical concentration used for the study was 100.8, 1,008 and 10,000 mg/l, respectively (nominal concentrations). Mineral nutrient medium containing Fe complex and additional trace elements was prepared in ultrapure water. Solid sodium hydrogen carbonate was added, to give twice the concentration indicated in the OECD guideline. This solution, at pH 8.15 after equilibration with air, was sterilized by ultrafiltration through a 0.45µ membrane filter before the test. The incubation of the test and control solutions was carried out for 72 hrs. The solutions were stirred for 15 minutes in every hour with a magnetic stirrer, to ensure adequate CO2 input. The test solutions were covered with a Plexiglas lid, to maintain sterile conditions. Based on the effect on the growth rate of the test organism Scenedesmus subspicatus, the 72 hr NOEC, EC10 and EC50 value was determined to be > 10,000 mg/l. Thus, based on the EC50 value, it can be concluded that the substance C.I. Pigment Black 7can be considered as non-toxic to Scenedesmus subspicatus at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.
Thus, based on the overall reported results for target chemical 2 -[hydroxy(methyl)amino]ethanol (OECD QSAR toolbox version 3.3 and EPI suite, 2017) and for its read across substance (from secondary source OECD SIDS), it can be concluded that the test substance 2 -[hydroxy(methyl)amino]ethanol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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