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EC number: 220-076-0 | CAS number: 2623-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 26, 1999 - Oct 29, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixed population of aquatic microorganisms
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent of a domestic sewage treatment plant (Kläranlage Odenthal)
- Storage conditions: not reported
- Storage length: not reported
- Preparation of inoculum for exposure: separation of coarse particles by filtration
- Pretreatment: none
- Preconditioning: aeration for 5 days
- Water filtered: not reported - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: not reported
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 20 ± 1 °C
- pH: not reported
- pH adjusted: no
- Aeration of dilution water: not reported
- Suspended solids concentration: not reported
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: aeration of inoculum prior to test
- Measuring equipment: not reported
- Test performed in closed vessels: yes
- Test performed in open system: no
SAMPLING
- Sampling frequency: day 0, 7, 14, 21, 28
- Sampling method: two vessels per sampling day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 48
- Sampling time:
- 28 d
- Details on results:
- 48% of the test substance were degraded after 48 days. The test substance was not readily biodegradable under the conditions of the test. The test substance was not inhibitory at tested concentrations of 2.6 mg/L.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable, not fulfilling specific criteria
- Conclusions:
- The test substance is regarded as not readily biodegradable but considered to be inherently biodegradable, not fulfilling specific criteria.
- Executive summary:
The ready biodegradability of the substance was studied under GLP conditions in the closed bottle test according to EU Method C.4-E over a period of 28 days. The inoculum was taken from runoff of a municipal sewage treatment plant, filtered and aerated for five days prior to the test. 2.6 mg/L test substance was suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms and incubated for 28 days under aerobic conditions in the dark at 20 ± 1°C. The dissolved oxygen concentration was measured in two test bottles at the beginning of the test and in another two testing vessels at a frequency of once per week. A fraction of 48% of the substance had been degraded at the end of the observation period. A parallel degradation test was conducted with sodium benzoate, which resulted in a fraction of 81% of the reference substance being biodegraded showing the suitability of the inoculum. A toxicity control containing both test and reference substance was tested in parallel showing that the test substance was not inhibitory to the inoculum. In conclusion, the substance must be regarded as not readily biodegradable under the conditions of this test. But can be regarded as biodegradable as it degraded between 40 and 60 %, which is a clear indication that extensive primary biodegradation has occured.
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- QSAR prediction: migrated from IUCLID 5.6
- Principles of method if other than guideline:
- Calculation based on BIOWIN v4.10, Estimation Programs Interface Suite™ for Microsoft® Windows v 4.11. US EPA, United States Environmental Protection Agency, Washington, DC, USA.
- GLP compliance:
- no
- Details on results:
- For detailed description on the model and its applicability, see "Any other information on materials and methods incl. tables".
- Conclusions:
- The substance is likely to be biodegradable in the environment and not fulfilling the criteria for environmental persistence. It may, however, not be readily biodegradable.
- Executive summary:
According to the ECHA Guidance document on the PBT assessment R.12 (ECHA 2008), the results from freely available estimation models, such as BIOWIN 2, 3 and 6, can be used to make assumptions on the potential environmental persistence of a substance when considered in a combined way. The guidance gives the following criteria for non-degradability: a) the result of the non-linear model prediction with BIOWIN 2 is < 0.5 and the ultimate timeframe prediction with BIOWIN 3 is < 2.2 (or 2.7); b) the MITI non-linear model prediction with BIOWIN 6 is < 0.5 and ultimate timeframe prediciton with BIOWIN 3 is < 2.2 (or 2.7).
The predictions for the substance menthyl acetate were 0.9862 with BIOWIN 2, 2.9011 with BIOWIN 3 and 0.3884 with BIOWIN 6. According to these model predictions, the substance is likely to be biodegraded in the environment and not fulfilling the criteria for environmental persistence. It may, however, not be readily biodegradable.
Referenceopen allclose all
Details of the degradation test with the test substance
Test substance Test concentration: 2.6 mg/L ThOD: 2663 mg/g COD: -- mg/g |
DO depletion after n days [mg/L] |
|||
7 d |
14 d |
21 d |
28 d |
|
No.1: (mt0 - mtx) -(mb0 - mbx) |
1.13 |
1.36 |
2.55 |
3.23 |
No.2: (mt0 - mtx) -(mb0 - mbx) |
1.06 |
1.50 |
2.42 |
3.37 |
Dl: % Degradation |
16 |
20 |
37 |
47 |
Dl: % Degradation |
15 |
22 |
35 |
49 |
D, Mean |
16 |
21 |
36 |
48 |
Details of the degradation tests with the reference substance
Reference substance Test concentration: 2.9 mg/L ThOD: 1665 mg/g COD: -- mg/g |
DO depletion after n days [mg/L] |
|||
7 d |
14 d |
21 d |
28 d |
|
No.1: (mt0 - mtx) -(mb0 - mbx) |
3.17 |
3.74 |
3.72 |
3.84 |
No.2: (mt0 - mtx) -(mb0 - mbx) |
3.15 |
3.79 |
3.63 |
3.89 |
Dl: % Degradation |
66 |
77 |
77 |
80 |
Dl: % Degradation |
65 |
78 |
75 |
81 |
D, Mean |
66 |
78 |
76 |
81 |
Details of the degradation test with the toxicity control
Toxicity control (2.6 mg/L test substance and 2.9 mg/L reference substance)
|
DO depletion after n days [mg/L] |
|||
7 d |
14 d |
21 d |
28 d |
|
No.1: (mt0 - mtx) -(mb0 - mbx) |
4.05 |
4.94 |
5.45 |
6.59 |
No.2: (mt0 - mtx) -(mb0 - mbx) |
3.86 |
5.06 |
5.29 |
6.42 |
Dl: % Degradation |
34 |
42 |
46 |
56 |
Dl: % Degradation |
33 |
43 |
45 |
55 |
D, Mean |
34 |
43 |
46 |
56 |
SMILES : O=C(C)OC1CC(C)CCC1C(C)C
MOL FOR: C12 H22 O2
MOL WT : 198.31
--------------------------- BIOWIN v4.10 Results ----------------------------
Biowin1 (Linear Model Prediction) : Biodegrades Fast
Biowin2 (Non-Linear Model Prediction): Biodegrades Fast
Biowin3 (Ultimate Biodegradation Timeframe): Weeks
Biowin4 (Primary Biodegradation Timeframe): Days
Biowin5 (MITI Linear Model Prediction) : Readily Degradable
Biowin6 (MITI Non-Linear Model Prediction): Not Readily Degradable
Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast
Ready Biodegradability Prediction: YES
----------------------------------------------
Biowin1 (Linear Biodeg Probability): 0.8273
Biowin2 (Non-Linear Biodeg Probability): 0.9862
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
----------------------------------------------
Biowin3 (Survey Model - Ultimate Biodeg): 2.9011
Biowin4 (Survey Model - Primary Biodeg): 3.7906
Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks (Primary & Ultimate) 2.00 -> months 1.00 -> longer
----------------------------------------------
Biowin5 (MITI Linear Biodeg Probability): 0.5134
Biowin6 (MITI Non-Linear Biodeg Probability): 0.3884
A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable A Probability Less Than 0.5 indicates --> NOT Readily Degradable
----------------------------------------------
Biowin7 (Anaerobic Linear Biodeg Prob): 0.2821
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
----------------------------------------------
Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey model) result is "weeks" or faster (i.e. "days", "days to weeks", or "weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then the prediction is YES (readily biodegradable). If this condition is not satisfied, the prediction is NO (not readily biodegradable). This method is based on application of Bayesian analysis to ready biodegradation data (see Help). Biowin5 and 6 also predict ready biodegradability, but for degradation in the OECD301C test only; using data from the Chemicals Evaluation and Research Institute Japan (CERIJ) database.
Description of key information
Inherently biodegradable, not fulfilling specific criteria: 48% (O2 consumption) after 28 days (EU Method C.4-E); read-across
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
Additional information
Since no data for the target substance (L-menthyl acetate, CAS 2623-23-6) are available, the biodegradability is assessed on the basis of data from one structurally similar substance (stereo isomers). This read-across is in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5. The target substance L-menthylacetate is a mono-constituent substance. This allows the use of experimental data from a racemic mixture (D- and L-isomers) of menthylacetate for the assessment of the target substance. A detailed justification of the analogue approach is provided in the technical dossier (see IUCLID Section 13).
The key study with the source substance was performed as a closed bottle test according to EU Method C.4-E (GLP). Secondary effluent from a domestic sewage treatment plant treating predominantly domestic sewage was used as inoculum to determine the biodegradation of the substance. A substance concentration of 2.6 mg/L was added to the test vessels. Moreover, a toxicity control containing both, test substance and reference substance, was included in the study to assess possible toxic effects of the substance to the inoculum. The degradation rate was determined based on the measurement of the oxygen consumption. After 28 d a significant fraction of 48% was degraded (clear indication that extensive primary degradation occured) and biodegradation was still ongoing. Therefore, the substance is partly but not readily biodegradable according to the OECD criteria and menthyl acetate was classified to be “inherently biodegradable, not fulfilling specific criteria”.
A supporting QSAR estimation was performed for the target substance itself. According to the ECHA Guidance document on the PBT assessment R.12 (ECHA, 2008), the results from freely available estimation models, such as BIOWIN 2, 3 and 6, can be used to make assumptions on the potential environmental persistence of a substance if considered in a combined way. The guidance gives the following criteria for non-degradability: a) the result of the non-linear model prediction with BIOWIN 2 is < 0.5 and the ultimate timeframe prediction with BIOWIN 3 is < 2.2 (or 2.7); b) the MITI non-linear model prediction with BIOWIN 6 is < 0.5 and ultimate timeframe prediction with BIOWIN 3 is < 2.2 (or 2.7). The predictions for the target substance were 0.9862 with BIOWIN 2, 2.9011 with BIOWIN 3 and 0.3884 with BIOWIN 6. According to these model predictions, the substance is likely to be biodegraded in the environment and not fulfilling the criteria for environmental persistence. It may, however, not be readily biodegradable.
Based on the results from one structurally related source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be concluded that the target substance (L-menthyl acetate) is not readily biodegradable according to the OECD criteria but is considered as inherently biodegradable. Additional (Q)SAR estimations for the target substance indicate that the substance is not likely to be persistent under environmental conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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