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EC number: 216-088-0 | CAS number: 1493-27-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 04 to 13 May 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Although the test was performed according to an internationally recognized guideline (but not under GLP compliance), no information was available about the purity of the test substance. There was no analytical monitoring performed during the study. However, the available water solubility data indicates that 2-fluoronitrobenzene is soluble (water solubility = 1607 mg/L, see IUCLID section 4.8) and thus, there should have been no solubility issue at the concentrations tested during this ecotoxicity study (maximum 100 mg/L).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- 1-fluoro-2-nitrobenzene
- EC Number:
- 216-088-0
- EC Name:
- 1-fluoro-2-nitrobenzene
- Cas Number:
- 1493-27-2
- Molecular formula:
- C6H4FNO2
- IUPAC Name:
- 1-fluoro-2-nitrobenzene
- Test material form:
- liquid
- Details on test material:
- Identity: 2-FLUORONITROBENZENE
CAS number: 1493-27-2
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test solutions were prepared by direct dispersion into test medium.
- Controls: Yes (test medium without test item).
No further data.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 270 mg/L CaCO3
- Test temperature:
- 21°C
- pH:
- 7.8 +/- 0.2 adjusted if necessary with NaOH or HCl (no information on whether such adjustment was actually performed)
- Nominal and measured concentrations:
- Nominal: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels: 200 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was prepared as follows:
a) CaCl2.2H2O 11.76 g/L
b) MgS04.7H2O 4.93 g/L
c) NaHC03 2.59 g/L
d) KCl 0.23 g/L
25 mL of each of solutions a) to d) were added to deionised water (conductivity < 5 µS/cm) to reach a final volume of 1 L.
EFFECT PARAMETERS MEASURED
Immobilization after 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: Yes
Test concentrations: 0, 0.1, 1, 10 and 100 mg/L
Results used to determine the conditions for the definitive study: 100 % mortality at 100 mg/L and 70% at 10 mg/L. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Other effect concentrations:
24h-EC50 = 26 mg/L.
24h-NOEC = 18 mg/L
48h-NOEC = 5.6 mg/L. - Reported statistics and error estimates:
- Analysis of the immobility data was performed by the moving average method of Thompson (1947).
Any other information on results incl. tables
Cumulative immobilisation data for Daphnia magna exposed for 48 hours to 2-fluoronitrobenzene
Nominal Concentration (mg/L) |
Cumulative immobilized Daphnia (initial population: 10 per replicate) |
|||||||
24 hours |
48 hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5.6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
6 |
8 |
14 |
70 |
18 |
0 |
0 |
0 |
0 |
9 |
8 |
17 |
85 |
32 |
8 |
9 |
17 |
85 |
10 |
10 |
20 |
100 |
56 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
100 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- The criterion according to which there should be no immobility in the controls is fulfilled, but there is no information reported regarding the dissolved oxygen concentrations at the end of the test (which should be > 3 mg/L).
- Conclusions:
- A 48h-EC50 (Daphnia magna) of 9.7 mg/L was determined; indicating that 2-fluoronitrobenzene is toxic to aquatic invertebrates.
- Executive summary:
The acute toxicity of 2-fluoronitrobenzene to Daphnia magna was evaluated according to the OECD 202 guideline, but not under GLP conditions. Groups of 10 daphnids were exposed for 48 hours under static conditions to a control treatment and to 2-fluoronitrobenzene concentrations of 1, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L in duplicates. Mobility was observed after 24 and 48 hours. There was no analytical monitoring of the test item concentrations during the exposure. The 48h-EC50 was set equal to 9.7 mg/L; indicating that 2-fluoronitrobenzene was toxic to aquatic invertebrates under the tested experimental conditions.
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