α-chloro-4-fluoroacetophenone

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11.12.1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: German national standard method with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard guideline: DIN 38412 part 27 (Draft)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

α-chloro-4-fluoroacetophenone

EC Number:

207-256-4

EC Name:

α-chloro-4-fluoroacetophenone

Cas Number:

456-04-2

Molecular formula:

C8H6ClFO

IUPAC Name:

2-chloro-1-(4-fluorophenyl)ethan-1-one

Details on test material:

- Name of test material (as cited in study report): p-Fluoro-w-chloroacetophenone
- Analytical purity: > 99%
- Physical state: solid
- Lot/batch No.: 23473/86/2

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: The test strain of Pseudomonas putida DSM 50026 used is obtained at regular intervals from DSM (German Collection of Microorganism) and is kept on agar slants for further culturing in the Ecotoxicology Laboratory of BASF AG Ludwigshafen .
- Method of cultivation:
- pre-culture
-vessel: Erlenmeyer flask
-volume: 500 mL
-inoculating density: streak with inoculating loop
-incubation time: 16 ± 2 h with shaking
-adjustment of the bacteria suspension: Centrifuge at 6000 rpm for 10 min, decant supernatant, resuspend bacteria in buffer solution and centrifuge again as stated above. Repeat washing and centrifuging. Resuspend bacteria with as little buffer solution as possible.

- Pretreatment: The concentration of the glucose stock solution is 49.54 g/100 mL water ; dissolution time 2 hours. 2 mL of this solution are added to each test and control mix.

- Initial biomass concentration: 240 - 400 TE/F (Turbidity unit/Formazin)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

21 ± 1 °C

Details on test conditions:

TEST SYSTEM
-vessel: Erlenmeyer flask
-volume: 500 mL
-inoculating density: streak with inoculating loop
-incubation time: 16 ± 2 h with shaking
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Light intensity: daylight

EFFECT PARAMETERS MEASURED:
measurement of the oxygen content in the medium: 2 - 5 minutes (continuously)
cell counting: determination of the optical density of the pre-culture as validity criterion
pH-measurement: at the start of the test in all concentrations

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

970 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

other: EC90

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes