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EC number: 202-855-7 | CAS number: 100-47-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1961
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficent for assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
- Principles of method if other than guideline:
- Laboratory operations described in detail by Henderson and Tarzwell
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Lepomis macrochirus
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 24 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Duration:
- 48 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Sublethal observations / clinical signs:
Results for different bioassays use
and experimental conditions :
TLM / LC50 (mg/l)
Kind of Fish
Dilution Water
24 hour
48 hour
96 days
Bluegills
Soft
78.
78.
78.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- he 96-h TLm (median toxic level) of Benzonitrile for bluegill is 78 mg/L in soft water.
- Executive summary:
The toxicity of benzonitrile for bluegill was tested under static condictions for groups of ten fish each at concentrations of
10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under soft water conditions.
The 96-h TLm (median toxic level) of Benzonitrile for bluegill was determined to be 78 mg/L in soft water.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1961
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient for assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
- Principles of method if other than guideline:
- Laboratory operations described in detail by Henderson and Tarzwell
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 24 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 116 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: hard water
- Duration:
- 24 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 240 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Duration:
- 48 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: hard water
- Duration:
- 48 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: hard water
- Duration:
- 96 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 135 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Sublethal observations / clinical signs:
Results for different bioassays use
and experimental conditions :
TLM / LC50 (mg/l)
Kind of Fish
Dilution Water
24 hour
48 hour
96 days
Fathead minnow
Hard
116.
78.
78.
Fathead minnow
Soft
240.
180.
135.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 96-h TLm (median toxic level) of Benzonitrile for fathead minnow is 78 mg/L in hard water and 135 mg/L in soft water.
- Executive summary:
The toxicity of benzonitrile for fathead minnow was tested under static condictions for groups of ten fish each at concentrations of
10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under hard water conditions as well as under soft water conditions.
The 96-h TLm (median toxic level) of Benzonitrile for fathead minnow was determined to be 78 mg/L in hard water and 135 mg/L in soft water.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1961
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficent for assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
- Principles of method if other than guideline:
- Laboratory operations described in detail by Henderson and Tarzwell
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Poecilia reticulata
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 24 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Duration:
- 48 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: TLm
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: soft water
- Sublethal observations / clinical signs:
Results for different bioassays use
and experimental conditions :
TLM / LC50 (mg/l)
Kind of Fish
Dilution Water
24 hour
48 hour
96 days
Guppies
Soft
400.
400.
400.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- he 96-h TLm (median toxic level) of Benzonitrile for guppy is 400 mg/L in soft water.
- Executive summary:
The toxicity of benzonitrile for guppy was tested under static condictions for groups of ten fish each at concentrations of
10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under soft water conditions.
The 96-h TLm (median toxic level) of Benzonitrile for guppy was determined to be 400 mg/L in soft water.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1982
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- abstract
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- no data; secondary reference
- GLP compliance:
- not specified
- Remarks:
- abstract from IFA GESTIS
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Brachydanio rerio (new name: Danio rerio) & Leuciscus idus
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 96 h
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 54 - 130 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks on result:
- other: median 92 mg/l; number of studies: 2
- Validity criteria fulfilled:
- not specified
- Conclusions:
- LC 50 (fish, 96 h) = 92 mg/l (median);
reported minimum value: LC50 = 54 mg/L - Executive summary:
A study conducted by Wellens, H. (1982: Comparison of the Sensitivity of Brachydanio rerio and Leuciscus idus by Testing the Fish Toxicity of Chemicals and Wastewaters. Z.Wasser-Abwasser-Forsch. 51(2):49-52.) is referenced in the GESTIS-database on hazardous substances (www.dguv.de/ifa/gestis-database). The reported median LC50 (96) for Brachydario rerio and Leuciscus idus is 92 mg/l. The LC50 ranges between 54 and 130 mg/L.
Referenceopen allclose all
Description of key information
The reported range of the 96-h LC50 of benzonitrile for different fish species is between 54 mg/L and 135 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 54 mg/L
Additional information
The toxicity of benzonitrile for fathead minnow, bluegill and guppy was tested under static conditions for groups of ten fish per concentration at levels of 10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The tests were conducted under soft water conditions for all three fish species and in addition under hard water conditions for fathead minnow.
The 96-h TLm (median toxic level, equivalent to LC50) of benzonitrile for fathead minnow was determined to be 78 mg/L in hard water and 135 mg/L in soft water.
The 96-h TLm for bluegill and guppy was determined to be 78 mg/L and 400 mg/L, respectively, in soft water.
A study conducted by Wellens, 1982 is referenced in the GESTIS-database on hazardous substances (www.dguv.de/ifa/gestis-database). The reported median LC50 (96) for Brachydario rerio and Leuciscus idus is 92 mg/l. The minimum value determined in the study is 54 mg/L (species not reported).
As the value of 54 mg/L is the most sensitive value reported in all available studies, this value is used as key value for the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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