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EC number: 217-157-8 | CAS number: 1758-73-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Reference Type:
- secondary source
- Title:
- Methanesulfinic acid, Aminoimino, CAS No: 1758-73-2
- Author:
- OECD SIDS
- Year:
- 2 002
- Bibliographic source:
- OECD SIDS; UNEP Publications
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Version / remarks:
- adopted in 1984
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Central Veterinary Public Health Inspectorate (Section GLP), Rijswijk, The Netherlands
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Aminoiminomethanesulphinic acid
- EC Number:
- 217-157-8
- EC Name:
- Aminoiminomethanesulphinic acid
- Cas Number:
- 1758-73-2
- Molecular formula:
- CH4N2O2S
- IUPAC Name:
- aminoiminomethanesulphinic acid
- Test material form:
- solid
Constituent 1
Method
- Target gene:
- HGPRT-locus
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Ham´s F-12 culture medium, supplemented with heat-inactivated foetal calf serum (10% v/v), L-glutamine (2 mM) and gentamicin (50 mg/L) (growth medium)
- Properly maintained: yes- Periodically checked for Mycoplasma contamination: no
- Periodically checked for karyotype stability: yes/no
- Periodically "cleansed" against high spontaneous background: no
- Metabolic activation:
- with and without
- Metabolic activation system:
- co-factor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- Experiment I
Without and with S9 mix: 4.75, 13.7, 41.2,124, 370, 1111, 3333 and 10000 µg/mL (4 h)
Experiment II
Without S9 mix: 250, 500, 750, 1000, 1100, and 1200 µg/mL (4 h)
With S9 mix: 250, 500, 1000, 1250, 1500, 1750, 2000 and 2500 µg/mL (4h) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ham´s F12-culture medium
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- other: dimethylnitrosamine and dimethylbenzanthracene
- Remarks:
- Ethylmethanesulfonate (EMS), 500 µg/mL culture medium, with S9; dimethylbenzanthracene (DMBA), 20 µg/mL, without S9 and dimethylnitrosamine (DMN) 3000 µg/mL
- Details on test system and experimental conditions:
- - Exposure duration: 4 h
- Expression time (cells in growth medium): about 24 h after addition of the test substance (day 1 of the study) cell cultures were trypsinized and counted. The initial survival of the cell was measured (Day 1 of the study). Furthermore, one 75 cm² tissue culture flask containing 1500000 (2 day culture period) or 750000 cells (3 day culture period) was prepared from the cell suspensions of each concentration level. The cells were subcultured at 2 or 3 day intervals during the expression period (till Day 8 of the study).
- Selection time (if incubation with a selection agent): Day 8
- Fixation time (start of exposure up to fixation or harvest of cells): Day 8
SELECTION AGENT: 6-TG
STAIN: 4% Giemsa
DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency - Evaluation criteria:
- The following criteria were used to evaluate the data obtained in the HGPRT assay:
a) the survival (absolute cloning efficiency) of negative controls should not be less than 50%<,
b) the mean mutant frequency of the negative control should fall within the range of 0 to 15 6-TG resistance mutants per 10E6 clonable cells
c) the mean mutant frequency of the positive controls should be higher than 20 6-TG resistant mutants per 10E6 clonable cells, and should at least be 5 times higher than the corresponding negative control
d) the highest test substance concentration should, if possible, result in a clear cytotoxic response (e.g: 10-30% of the relative initial survival). Any apparent increased in mutant frequency at concentrations of the test substance causing more than 90% toxicity is considered to be an artefact and not indicative of genotoxicity
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment I: - S9 at 1111.1 µg/mL; +S9: 3333.3 and 10000 µg/mL; Experiment II: at 1200 and 2500 µg/mL (+ and - S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: Chinese hamster Ovary (CHO)
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Experiment I - 4 h exposure - Without Metabolic Activation
Concentration |
Cloning efficiency [%] |
Mutants frequency per 1E+06 clonable cells |
0 |
104.4 |
1.9 |
4.75 |
100.5 |
0.5 |
13.7 |
99.2 |
0.0 |
41.2 |
101.7 |
1.5 |
124 |
92.1 |
0.0 |
370 |
99.5 |
1.0 |
1111 |
94.8 |
6.3 |
3333 |
-- |
-- |
10000 |
-- |
-- |
EMS 500 |
82.7 |
205.0 |
EMS ethymethanesulfonate
Table 2: Experiment I - 4 h exposure - With Metabolic Activation
Concentration |
Cloning efficiency [%] |
Mutants frequency per 1E+06 clonable cells |
0 |
102.8 |
1.5 |
4.75 |
107.6 |
2.3 |
13.7 |
98.5 |
2.0 |
41.2 |
103.9 |
1.0 |
124 |
98.3 |
3.1 |
370 |
102.1 |
2.9 |
1111 |
94.4 |
2.6 |
3333 |
N.D (*) |
-- |
10000 |
-- |
-- |
DMN 3000 |
74.5 |
93.3 |
DMHA 20 |
92.2 |
160.0 |
DMN dimethylnitrosamine
DMBA dimethylbenzanthracene
N.D=not determined, because no cells were recovered after treatment of (*) because of a limited number of cells were recovered for cloning efficiency
Table 3: Experiment II - 4 h exposure - Without Metabolic Activation
Concentration |
Cloning efficiency [%] |
Mutants frequency per 1E+06 clonable cells |
0 |
93.3 |
3.8 |
250 |
101.1 |
0.0 |
500 |
96.4 |
0.0 |
750 |
93.5 |
0.0 |
1000 |
93.6 |
0.0 |
1100 |
95.0 |
2.1 |
1200 |
97.2 |
1.0 |
EMS 500 |
78.9 |
67.2 |
EMS ethymethanesulfonate
Table 4: Experiment II - 4 h exposure – With Metabolic Activation
Concentration |
Cloning efficiency [%] |
Mutants frequency per 1E+06 clonable cells |
0 |
97.0 |
1.5 |
250 |
103.2 |
0.5 |
500 |
104.3 |
6.7 |
1000 |
96.0 |
3.1 |
1250 |
93.9 |
3.7 |
1500 |
95.4 |
1.6 |
1750 |
91.4 |
3.8 |
2000 |
91.2 |
3.3 |
2500 |
75.2 |
2.0 |
DMBA 20 |
85.5 |
164.9 |
DMBA dimethylbenzanthracene
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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