Chloroacetaldehyde

Short-term toxicity to fish

Description of key information

Mature zebrafishes (Brachydanio rerio) commercially purchased were housed separated according to sex in aquaria for producing mature artemia larvas. Four female and eight male fishes were kept together overnight for spawing at dawn simulated by neon lighting. The eggs were fertilized extracorporally by the delivered sperm of the male fishes. The spawn was collected on the bottom of a glass box covered by a steel wire-net containing some small green glass trees. The eggs were separated and cultured at 25°C individually using micro plates containing 100uL reconstituted water.
At the 8-cell stage, test substances in 100/uL reconstituted water each were added after neutralization to pH 8 by HCI. Each test contained one control and up to seven concentrations of each chemical; 12 eggs were used per concentration or control. The eggs were observed microscopically for about 96 hr until hatching. Developmental stages were determined as described elsewhere (Hisaoka and Battle 1958). Marked signs of development, e.g. abnormalities, and deaths were recorded.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

42.7 mg/L