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EC number: 700-626-0 | CAS number: 1073607-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14,2000.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[(1E)-2-[(3Z)-2-chloro-3-{2-[(2E)-1,1-dimethyl-3-(4-sulfobutyl)-1H,2H,3H-benzo[e]indol-2-ylidene]ethylidene}cyclohex-1-en-1-yl]ethenyl]-1,1-dimethyl-3-(4-sulfonatobutyl)-1H-benzo[e]indol-3-ium amine
- EC Number:
- 700-626-0
- Cas Number:
- 1073607-50-7
- Molecular formula:
- C46 H50 Cl N2 O6 S2 . H4 N
- IUPAC Name:
- 2-[(1E)-2-[(3Z)-2-chloro-3-{2-[(2E)-1,1-dimethyl-3-(4-sulfobutyl)-1H,2H,3H-benzo[e]indol-2-ylidene]ethylidene}cyclohex-1-en-1-yl]ethenyl]-1,1-dimethyl-3-(4-sulfonatobutyl)-1H-benzo[e]indol-3-ium amine
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Aanlysis performed at the start and after 48h of exposure.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with a loading rate of 100mg/l applying a 15-minute period of ultrasonic treatment in combination with stirring. Thereafter, the aqueous dispersion was magnetically stirred for three days to ensure reaching maximum dissolution in test medium. The resulting green dispersion contained precipitate and floating small particles at the surface and through the dispersion. The dispersion was consequently filtered througha 0.45µm membrane filter to remove the undissolved material. This resulted in a very slightly green filtrate that was used as highest test concentration. The lower test concentrations were prepared by subsequent dilutions of the filtrat in test medium. The dilutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180mg/l expressed as CaCO3
- Test temperature:
- 18-22°C
- pH:
- 7.5-7.9
- Dissolved oxygen:
- 8.7-8.8mg/l
- Salinity:
- The test medium is an adjusted ISO medium made by dissolving several macro salts, trace elements, macro nutrients and vitamins to tap water purified by reverse osmosis (medium called M7, as presribed by Dr. Elendt-Schneider: Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33)
- Nominal and measured concentrations:
- Analysis of duplicate samples taken from the filtered solution at the start of the test showed a measured concentration of 0.2mg/l. Measured concentrations in the dilutions were all below the limit of quantification. During the exposure period the measured concentration in the filtered solution decreased below the limit of quantification, i.e. below 0.1mg/l. This was expected to be due to the very low solubility in test medium, i.e. the start concentrations might stil be above the limit of solubility.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.32 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility (including mortality)
- Remarks on result:
- other: The initial measured exposure concentration corresponds to a loading concentration of >100mg/l.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.32 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility (including mortality)
- Remarks on result:
- other: The initial measured exposure concentration corresponds to a loading concentration of >100mg/l.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility (including mortalilty)
- Remarks on result:
- other: The initial measured exposure concentration corresponds to a loading concentration of 100mg/l.
Any other information on results incl. tables
Acceptability of the test:
1.. In the control, no daphnids became immobilised.
2. The oxygen concentration at the end of the test was >+ 3mg/l in control and test vessels.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 exceeded an initial measured concentration of 0.32mg/l being the maximum soluble concentration of the test substance in test medium.
Due to the very low solubility in test medium concentrations toxic for Daphnia magna could not be reached.
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