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EC number: 218-792-3 | CAS number: 2235-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Test chemical did not induce mutation in with and without S9 mix and hence it is not likely to classify as a gene mutant in vitro.
hence it is not likely to classify as a gene mutant in vitro.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across substances
- Justification for type of information:
- Weight of evidence approach based on structurally similar chemicals
- Reason / purpose for cross-reference:
- exposure-related information
- Reason / purpose for cross-reference:
- exposure-related information
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- WoE report is based on two gene toxicity studies 1. and 2 Genotoxicity was performed to determine the mutagenic nature of test chemical
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Species / strain / cell type:
- other: S. typhimurium strains TA98, 100, 1535, 1537 and 1538
- Details on mammalian cell type (if applicable):
- not specified
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium, other: Salmonella Typhimurium TA98, TA100, TA1535, TA1537, TA97
- Details on mammalian cell type (if applicable):
- not specified
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- not specified
- Test concentrations with justification for top dose:
- 2. 10 mg/Platte
- Vehicle / solvent:
- not specified
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Remarks:
- not specified
- Details on test system and experimental conditions:
- not specified
- Rationale for test conditions:
- not specified
- Evaluation criteria:
- 1. Revertants / plate (less than doubling)
- Statistics:
- not specified
- Species / strain:
- other: S.typhimurium strains TA98, 100, 1535, 1537 and 1538
- Remarks:
- 1.
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium, other: Salmonella Typhimurium TA98, TA100, TA1535, TA1537, TA97
- Remarks:
- 2.
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutation
- Conclusions:
- Test chemical did not induce mutation in with and without S9 mix and hence it is not likely to classify as a gene mutant in vitro.
- Executive summary:
AMES test was performed to determine the mutagenic nature of test chemical. The study was performed usingS. typhimuriumstrains TA98, 100, 1535, 1537 and 1538 both in the presence and absence of a metabolic activation system (liver S9 mix produced from rats pretreated with Aroclor 1254). The test material did not produce a statistically significant increase in the revertants / plate (less than doubling) thereby demonstrating no potential to induce gene mutations. Therefore, Test chemical did not induce revertants / plate (less than doubling) inS. typhimuriumstrains TA98, 100, 1535, 1537 and 1538 and hence it is not likely to classify as a gene mutant in vitro.
AMES test was performed to determine the mutagenic nature of test chemical. The study was performed using Salmonella Typhimurium TA98, TA100, TA1535, TA1537 and TA97 both in the presence and absence of a metabolic activation system at 10 mg/Platte. The test material did not induce gene mutations. Therefore, Test chemical did not induce mutation in Salmonella Typhimurium TA98, TA100, TA1535, TA1537 and TA97 and hence it is not likely to classify as a gene mutant in vitro.
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across substances
- Justification for type of information:
- Weight of evidence approach based on structurally similar chemicals
- Reason / purpose for cross-reference:
- exposure-related information
- Reason / purpose for cross-reference:
- exposure-related information
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Genotoxicity was performed to determine the mutagenic nature of test chemical
- GLP compliance:
- not specified
- Type of assay:
- other: 1. Genotoxicity was performed to determine the mutagenic nature of test chemical 2. Chinese Hamster lung fibroplast cells, V79
- Species / strain / cell type:
- other: Cell line
- Remarks:
- 1.
- Details on mammalian cell type (if applicable):
- not specified
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Details on mammalian cell type (if applicable):
- not specified
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with
- Metabolic activation system:
- liver S9 mix produced from rats pretreated with Aroclor 1254
- Test concentrations with justification for top dose:
- not specified
- Vehicle / solvent:
- not specified
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Remarks:
- not specified
- Details on test system and experimental conditions:
- not specified
- Rationale for test conditions:
- not specified
- Evaluation criteria:
- not specified
- Statistics:
- not specified
- Species / strain:
- other: cell line
- Remarks:
- 1.
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Remarks:
- 2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutation
- Conclusions:
- Test chemical did not induce mutation in with and without S9 mix and hence it is not likely to classify as a gene mutant in vitro.
- Executive summary:
SCE-assay was performed to determine the mutagenic nature of test chemical. The study was perfomed using SCE cell both in the presence and absence of a S9 metabolic activation system (liver S9 mix produced from rats pretreated with Aroclor 1254). Highest test substance concentrations employed in this test were 0.1 and 0.3 mg/ml and exposure times were 5h and 2 h in the absence and presence of a metabolic activation system, respectively. Additionally, it was found that 2,4-pentanedione was considered genotoxic particularly in the absence of metabolic activation since the magnitude of SCE induction was lower when activation by S9 mix was included. Therefore, Test chemical did not induction in SCE with metabolic activation and produce induction without S9 mix and hence it is not likely to classify as a gene mutant in vitro.
Genotoxicity was performed to determine the mutagenic nature of test chemical. The study was performed using Chinese Hamster lung fibroplast cells, V79 absence of a S9 metabolic activation system. No inhibition of intracellular communication was observed in Chinese Hamster lung fibroplast cells, V79. Therefore, Test chemical did not induction in Chinese Hamster lung fibroplast cells, V79 without metabolic activation and hence it is not likely to classify as a gene mutant in vitro.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Gene mutation in vitro:
Data available for the target chemical was reviewed to determine the mutagenic nature of the test chemical barium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate (CAS no 17852-98-1)The studies are as mentioned below:
Ames test:
AMES test was performed to determine the mutagenic nature of test chemical. The study was performed usingS. typhimuriumstrains TA98, 100, 1535, 1537 and 1538 both in the presence and absence of a metabolic activation system (liver S9 mix produced from rats pretreated with Aroclor 1254). The test material did not produce a statistically significant increase in the revertants / plate (less than doubling) thereby demonstrating no potential to induce gene mutations. Therefore, Test chemical did not induce revertants / plate (less than doubling) inS. typhimuriumstrains TA98, 100, 1535, 1537 and 1538 and hence it is not likely to classify as a gene mutant in vitro.
AMES test was performed to determine the mutagenic nature of test chemical. The study was performed using Salmonella Typhimurium TA98, TA100, TA1535, TA1537 and TA97 both in the presence and absence of a metabolic activation system at 10 mg/Platte. The test material did not induce gene mutations. Therefore, Test chemical did not induce mutation in Salmonella Typhimurium TA98, TA100, TA1535, TA1537 and TA97 and hence it is not likely to classify as a gene mutant in vitro.
Chromosome aberration:
SCE-assay was performed to determine the mutagenic nature of test chemical. The study was perfomed using SCE cell both in the presence and absence of a S9 metabolic activation system (liver S9 mix produced from rats pretreated with Aroclor 1254). Highest test substance concentrations employed in this test were 0.1 and 0.3 mg/ml and exposure times were 5h and 2 h in the absence and presence of a metabolic activation system, respectively. Additionally, it was found that 2,4-pentanedione was considered genotoxic particularly in the absence of metabolic activation since the magnitude of SCE induction was lower when activation by S9 mix was included. Therefore, Test chemical did not induction in SCE with metabolic activation and produce induction without S9 mix and hence it is not likely to classify as a gene mutant in vitro.
Genotoxicity was performed to determine the mutagenic nature of test chemical. The study was performed using Chinese Hamster lung fibroplast cells, V79 absence of a S9 metabolic activation system. No inhibition of intracellular communication was observed in Chinese Hamster lung fibroplast cells, V79. Therefore, Test chemical did not induction in Chinese Hamster lung fibroplast cells, V79 without metabolic activation and hence it is not likely to classify as a gene mutant in vitro.
Based on the data available from the test chemical and read across, N,N-Diethyl-3-oxobutyramide does not exhibit gene toxicity in in the presence and absence of metabolic activation.. Hence the substance cannot be classified as genetox in vitro.
Justification for classification or non-classification
Based on the data available from the test chemical and read across, N,N-Diethyl-3-oxobutyramide does not exhibit gene toxicity in in the presence and absence of metabolic activation.. Hence the substance cannot be classified as genetox in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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