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EC number: 464-520-3 | CAS number: 189813-45-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Remarks:
- BASF AG, Experimental Toxicology and Ecology
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): 520 F-Chlormethyloximether (Stufe 9 der BAS 520 FSynthese)
- Physical state: crystalline / grey
- Analytical purity: 98.3 g/100 g
- Lot/batch No.: 29388-136
- Storage condition of test material: room temperature
Method
- Target gene:
- histidine and tryptophan auxotrophy
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- 1st experiment, Standard plate test (with and without metabolic activation): 0, 20, 100, 500, 2500 and 5000 µg/plate
2nd experiment, Standard plate test (with and without metabolic activation): 0, 1000, 2000, 4000, 6000 and 8000 µg/plate
3rd experiment, Preincubation test (with and without metabolic activation): 0, 20, 100, 500, 2500 and 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Due to the insolubility of the test substance in water, DMSO was selected as the vehicle, which had been demonstrated to be suitable in bacterial reverse mutation tests and for which historical control data are available.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AA), 2.5-60 µg/ml for all strains
- Remarks:
- with S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N-nitro-N-nitrosoguanidine (MNNG), 5 µg/plate for TA1535 and TA100
- Remarks:
- without S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine (NOPD), 10 µg/plate for TA98
- Remarks:
- without S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9 mix
Migrated to IUCLID6: 100 µg/plate for TA1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- without S9 mix
Migrated to IUCLID6: 5 µg/ml for E. coli WP2 uvrA
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
DURATION
The experimental procedure of the Standard Plate Test (plate incorporation method) was based on the method of Ames et al (Mut. Res., 31 347 -364, 1975). The experimental procedure of the Preincubation Test was based on the method described by Yahagi et al. (Mut. Res., 48 121 - 130, 1977) and Matsushima et al. (In: Norpoth, K.H. and R.C. Garner, Short-Term Test Systems for Detecting Carcinogens. Springer Verlag Berlin, Heidelberg, New York, 1980).
NUMBER OF REPLICATIONS: 3
- Evaluation criteria:
- - The test chemical is considered positive in this assay if a dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strains either without S-9 mix or after adding a metabolizing system.
- A test substance is generally considered non-mutagenic in this test if the number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100, and E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 500 µg/plate onwards in the preincubation assay
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100, and E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 8000 µg/plate only using TA 100 in the standard plate test
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found
RANGE-FINDING/SCREENING STUDIES: Dose selection used in repeat studies or further experiments were based an the findings of the 1st experiment. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Expt. 1: Standard plate test: Mean Revertants/ plate (±SE)
Dose (µg/plate) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2uvrA |
|||||
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
|
0 |
30±4 |
41±6 |
141±9 |
147±3 |
17±1 |
21±2 |
11±2 |
9±2 |
27±4 |
33±3 |
20 |
24±7 |
32±2 |
141±12 |
151±10 |
20±5 |
20±4 |
8±2 |
9±1 |
28±7 |
29±6 |
100 |
23±3 |
33±7 |
146±14 |
173±37 |
22±2 |
23±4 |
8±3 |
13±1 |
28±3 |
39±2 |
500 |
24±4 |
26±5 |
139±11 |
154±8 |
28±3 |
19±2 |
10±3 |
11±2 |
29±4 |
34±2 |
2500 |
25±5 |
26±6 |
185±9 |
172±18 |
25±5 |
23±4 |
10±3 |
6±3 |
26±4 |
28±3 |
5000 |
18±2 |
24±3 |
181±15 |
187±28 |
22±4 |
16±4 |
9±3 |
6±2 |
28±7 |
24±3 |
PC |
984±58 |
969±69 |
641±48 |
1333±133 |
621±20 |
174±16 |
722±101 |
114±16 |
888±44 |
215±10 |
Expt. 2: Standard plate test: Mean Revertants/ plate (±SE)
Dose (µg/plate) |
TA 100 |
|
-S9 |
+S9 |
|
0 |
118±14 |
133±9 |
1000 |
134±16 |
144±17 |
2000 |
176±12 |
144±13 |
4000 |
126±15 |
168±21 |
6000 |
112±17 |
138±7 |
8000 |
79±20 |
91±3 |
PC |
729±114 |
1480±70 |
Expt. 3: Pre-incubation test: Mean Revertants/ plate (±SE)
Dose (µg/plate) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2uvrA |
|||||
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
|
0 |
34±3 |
28±3 |
124±5 |
117±9 |
18±1 |
18±4 |
9±3 |
12±2 |
23±3 |
27±2 |
20 |
29±4 |
32±3 |
105±11 |
114±15 |
19±3 |
21±4 |
9±2 |
12±3 |
21±7 |
24±5 |
100 |
26±2 |
27±3 |
119±10 |
102±10 |
20±1 |
17±1 |
10±1 |
9±3 |
19±2 |
23±3 |
500 |
27±6 |
24±4 |
92±5 |
78±3 |
21±5 |
13±2 |
9±1 |
10±2 |
22±3 |
17±5 |
2500 |
26±2 |
26±4 |
108±4 |
99±8 |
22±4 |
15±4 |
7±3 |
7±1 |
20±3 |
15±2 |
5000 |
19±2 |
23±2 |
84±1 |
110±5 |
19±2 |
13±1 |
6±2 |
6±1 |
14±5 |
17±4 |
PC |
894±94 |
1023±70 |
1626±55 |
649±43 |
1310±112 |
114±10 |
704±21 |
118±20 |
699±83 |
208±17 |
An increase in the number of his+ or trp+ revertants was not observed both in the standard plate test and in the pre-incubation test either without S-9 mix or after the addition of a metabolizing system. According to the results of the present study, the test substance 520 F-Chlormethyloximether (Stufe 9 der BAS 520 F-Synthese) is not mutagenic in the Salmonella typhimurium/ Escherichia coli reverse mutation assay under the experimental conditions chosen here.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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