dec-9-en-2-one

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Test dates: February 01 to 12, 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study was conducted according to an internationally recognised method, and under GLP. Deviations did not affect the validity of the study. The substance is adequately identified. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes (incl. QA statement)

Remarks:

GIPC; effective 16 March 2007 (validity 18 months)

Type of method:

flask method

Specific details on test material used for the study:

Storage conditions of test material: Stored at room temperature protected from direct sun light.

Key result

Water solubility:

272 mg/L

Conc. based on:

test mat.

Incubation duration:

24 - 72 h

Temp.:

20 °C

pH:

6.33

Estimated value

The hydrosolubility was first estimated using 20 µL of pure test substance in 10 mL of water in a 15 mL capacity glass tube. The tube was firmly shaken and then allowed to stand for 1 hour so that the phases separated. A 5 mL was then sampled from the water phase and extracted in 2 mL chloroform as validated.

The concentration was measured as 409 mg/L, so the main test was performed according to the flask method.

Measured value

8 tubes were rotated for 4 days at 20 +/-1°C. Four tubes were sampled at 24 h intervals and checked for that no particles may affect the results.

Day	mg/L (+/- SD, n=4)
1	286.6 (+/- 5.6)
2	256.2 (+/- 7.9)
3	273.6 (+/- 17.7)
4	274.5 (+/- 5.9)

The water solubility was calculated as mean from days 1, 2 and 3 which fulfilled the validity criteria: the standard deviation (6.4 mg/L) within the three successive values (287, 256 and 274 mg/L, rounded values) represented 2.4% of mean value.

Water solubility = 272 +/- 6 mg/L

pH of the water phase was 6.33 at the end of the test.

The equilibrium in water was achieved within 1 day, and the deletion of the incubation of the solutions at 30°C was justified.

Executive summary:

The water solubility of the test substance was determined under GLP based on EU A.6 guideline, flask method.

After preliminary testing, water was equilibrated with about 5 times the estimated substance amount, for 4 days, at 20°C. Concentrations were measured each day using a GC-FID validated method. Individual results fulfilled the validity criteria for reproducibility.

The water solubility of the test item was determined as 272 mg/L (at 20°C and pH=6.33).

Description of key information

Moderately soluble, with equilibrium reached within =< 24h. pH of saturated solution ca 6.3.

Key value for chemical safety assessment

Water solubility:

272 mg/L

at the temperature of:

20 °C

Additional information

A fully reliable experimental study, conducted according to a recognized EC method and under GLP, is available.

It is considered as a key study, and the result is retained as key data.