Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 445-090-6 | CAS number: 5614-37-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 November 2002 - 18 January 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD and EC test guidelines and in compliance with GLP, so the data is considered reliable without restrictions.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 92/69/EEC, C2
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Analytical purity: 99.9%
- Lot/batch No.: 020618 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations (nominally 10, 22, 46, 100, and 220 mg/L).
- Sampling method: Duplicate 30 mL samples taken from each test concentration and control at 0 hours (main stock solutions) and after 48 hours (replicates pooled). - Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: The test substance was directly spiked into Elendt M4 medium.
Preliminary investigations into the solubility and stability of the test substance indicated that it was volatile and readily lost from aqueous mixtures. Therefore, following the addition of the Daphnia, each test vessel was sealed with a screw-capped lid to prevent loss of material. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: Parthenogenesis
- Feeding during test
- Food type: Chlorella vulgaris
- Frequency: daily (during test organism culture; no suplementary feeding was given during the study period) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 to 21°C
- pH:
- 7.6 - 7.9
- Dissolved oxygen:
- 8.3 - 8.6 mg O2/L
- Nominal and measured concentrations:
- Nominal: 0, 10, 22, 46, 100, and 220 mg/L
Measured: 0 (no test item detected), 9.7, 20, 46, 100, 213 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass jars
- Type (delete if not applicable): closed (sealed to prevent loss of the volatile test material)
- Material, size, headspace, fill volume: 250 mL capacity / 250 mL fill volume.
- Aeration: No supplementary aeration during the test period
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 50 mL test solution per organism
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light / 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation; Daphnia were considered to be immobile if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Approximately 2.2
- Range finding study
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes. Preliminary study indicated that the EC50 was likely to lie between 10 and 100 mg/L. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 62 - 88 mg/L (95% CL)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 27 - 40 mg/L (95% CL)
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No Daphnia were immobilised in the control group
- Conclusions:
- The 48 hour EC50 (immobilisation) value for NZE with Daphnia magna was determined to be 35 mg/L.
The 'No-observed effect concentration' (NOEC) was 20 mg/L.
Reference
Description of key information
Daphnia Magna, EC50 (48 hours, immobilisation) = 35 mg/L
NOEC (48 hours) = 20 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 35 mg/L
Additional information
A determination of the acute toxicity to aquatic invertebrates of the substance was conducted (Huntingdon Life Sciences, 2003). The study was performed in accordance with EC method C2 and OECD test guideline 202, and in compliance with GLP. Groups of 20 Daphnia magna were exposed to 0 (control group), 10, 22, 46, 100, or 220 mg/L the substance in Elendt M4 Culture medium.
After 24 hours' exposure, all of the Daphnia in the 220 mg/L group, and 95% of the Daphnia in the 100 mg/L group were immobilised but no Daphnia in other groups were immobilised. After 48 hours, all of the Daphnia in the 100 mg/L group and 95% of the Daphnia in the 46 mg/L group were immobilised, but none of the Daphnia in the lower concentration groups were immobilised. The EC50 value and NOEC were expressed in terms of the mean measured concentration of the substance (mean of measurements at 0 hours and 48 hours).
It was concluded that the 48 -hour EC50 in Daphnia Magna was 35 mg/L. The no-observed effect concentration was concluded to be 20 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.