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EC number: 277-274-5 | CAS number: 73070-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/01/2006 - 05/01/2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: marine
- Details on inoculum:
- - Source of inoculum/activated sludge: Natural seawater collected from Anglesey Sea Zoo (North Wales), on collection the temperature was 15 °C, pH 7.95 and 100% dissolved oxygen.
- Storage conditions: Stored in darkness, 15 (+- 2) °C and gently aerated
- Water filtered: yes, coarse filtered - Duration of test (contact time):
- >= 0 - <= 28 d
- Initial conc.:
- 7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 9 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: nutrient fortified seawater
- Additional substrate: positive control included reference material (sodium benzoate)
- Test temperature: 18 - 19.5 °C
- pH: 7.95
- pH adjusted: no
- Aeration of dilution water: prior to definite test for aging only
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: BOD bottles of nominal 272 ml volume
- Number of culture flasks/concentration: 2 replicate flasks per time period tested per test material concentration (7 and 9 mg/ L). The same for the positive control (2.5 mg/ L sodium benzoate). For the blank control 3 replicates per time point measured were included.
- Test performed in open system: closed bottle method, sealed
SAMPLING
- Sampling frequency: 24 hour intervals
- Sampling method: dissolved oxygen content measured
CONTROL AND BLANK SYSTEM
- Inoculum blank: Nutrient fortified seawater
- Toxicity control: Nutrient fortified seawater with reference substance (sodium benzoate) - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The reference material had a degradation figure of > 100 % after 28 days demonstrating that the inoculum was biologically active.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 54.6
- Sampling time:
- 28 d
- Remarks on result:
- other: 7 mg/ L test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 41.7
- Sampling time:
- 28 d
- Remarks on result:
- other: 9 mg/ L test substance
- Details on results:
- Dissolved oxygen was measured for the test substance and the controls
- DOC 7.0 mg/ L test substance (average): 7.69, 6.82, 5.57, 4.72, 4.56 and 4.43 mg O2/ L for 0, 3, 7, 14, 21 and 28 days, respectively
- DOC 9.0 mg/ L test substance (average): 7.68, 6.40, 4.61, 3.40, 2.82 and 4.46 mg O2/ L for 0, 3, 7, 14, 21 and 28 days, respectively
- DOC 2.5 mg/ L sodium benzoate: 7.74, 3.61, 3.00, 2.82, 2.36 and 2.34 mg O2/ L for 0, 3, 7, 14, 21 and 28 days, respectively
- DOC blank (nutrient fortified seawater only): 7.68, 7.02, 6.55, 6.54, 6.35 and 6.50 mg O2/ L for 0, 3, 7, 14, 21 and 28 days, respectively - Key result
- Parameter:
- COD
- Value:
- 0.565 other: mg mg-1
- Results with reference substance:
- Reference substance (Sodium benzoate) had >100% degradability at 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The study showed 54.6% biodegradability for 7.0 mg/L test material and 41.7% biodegradability at 9 mg/ L test material at 28 days. These results show the test substance has good potential for biodegradation.
- Executive summary:
The study assesses the potential for 25% M528 -7 (W/W) neutralised with DEA in deionised water to readily biodegrade in water. The study was conducted under GLP in accordance with OECD guideline 306: Biodegradability in seawater, using a closed bottle method. All validity criteria were met. Testing was conducted using natural seawater that was coarse filtered, aged via aeration and darkness prior to testing and fortified with nutrients. The inoculum shown to have suitable biological activity based on the reference material results. Test substance experiments were performed with 2 replicates per concentration, per time period. For the degradability negative control dissolved oxygen was at an average of 7.68, 7.02, 6.55, 6.54, 6.35 and 6.50 mg O2/ L for 0, 3, 7, 14, 21 and 28 days (3 replicates). For the positive control (reference substance, sodium benzoate) the degradability was 83.1%, 86.5%, 90.5%, 97% and >100% for 3, 7, 14, 21 and 28 days (2 replicates).
The definitive test showed that at 7.0 mg/L the test substance readily biodegradability was 54.6% at day 28, while at the test concentration of 9 mg/L the readily biodegradability was 41.7% at Day 28. The results from this study show that the test substance show a good potential to biodegrade but it is not deemed to be readily biodegradable.
Reference
Description of key information
The study assesses the potential for 25% M528 -7 (W/W) neutralised with DEA in deionised water to readily biodegrade in water. The study was conducted under GLP in accordance with OECD guideline 306: Biodegradability in seawater, using a closed bottle method. All validity criteria were met. Testing was conducted using natural seawater that was coarse filtered, aged via aeration and darkness prior to testing and fortified with nutrients. The inoculum show to have suitable biological activity based on the reference material results. Test substance experiments were performed with 2 replicates per concentration, per time period. For the degradability negative control dissolved oxygen was at an average of 7.68, 7.02, 6.55, 6.54, 6.35 and 6.50 mg O2/ L for 0, 3, 7, 14, 21 and 28 days (3 replicates). For the positive control (reference substance, sodium benzoate) the degradability was 83.1%, 86.5%, 90.5%, 97% and >100% for 3, 7, 14, 21 and 28 days (2 replicates).
The test showed that at 7.0 mg/L the test substance readily biodegradability was 54.6% at day 28, while at the test concentration of 9 mg/L the readily biodegradability was 41.7% at Day 28. The results from this study show that the test substance show a good potential to biodegrade but it is not deemed to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
- Type of water:
- marine water
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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