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EC number: 205-858-1 | CAS number: 156-57-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2018 - May 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 23 march 2006
- Deviations:
- yes
- Remarks:
- The breeding of the organisms is carried out according to method APAT IRSA CNR 8020 appendix 1 . It is believed that these modifications have not had an effect on the quality and integrity of the study and the data produced
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 46, 15 e 10 mg/l
- Sampling method: aliquot were taken
- Sample storage conditions before analysis: refrigerated storage 4±2°C - Vehicle:
- no
- Details on test solutions:
- - Separation method : GC
- Conditions (column, mobile phase, etc.): solvent detection column (capillary column DB1, lenght: 60 m, int. diam. 0.53 mm)
- Detection method: FID
- Reproducibility in % (indicate method of evaluation; should be given for stated concentration levels): 20%
- Linearity range: 1-50 mg/l
- Extraction recovery (indicate if results are corrected or not for recoveries): not corrected
- Method of confirmation of identity of measured compound: standard (conc. 46 mg/l) - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Strain: PSEUDOKIRCHNERIELLA SUBCAPITATA (KORSHIKOV) F.HINDAK (1990)
- Source (laboratory, culture collection): Breeding of a certified Algaltox F kit
- Age of inoculum (at test initiation): 4/5 days
- Method of cultivation: APAT IRSA CNR 8020 appendix 1 .
ACCLIMATION
- Acclimation period: continuos breeding
- Culturing media and conditions (same as test or not): same as test excluding photoperiod (16 h light/ 8 h dark) and culturing media (see APAT IRSA CNR 8020 appendix 1) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20-24°C ( tmin 21.4°C; tmax 22.1°C);
- pH:
- 8.02÷8.43
- Nominal and measured concentrations:
- 1.5, 4.6, 6.8, 10 (measured 7.3 mg/l), 15 (measured 13.3 mg/l), 22, 46 mg/L (measured 47.8 mg/l)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flask
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 50 ml, sample 20 ml
- Aeration: no
- Initial cells density: 104 cell/ml
- Control end cells density: 1.64 x 106 cell/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: OECD TG 201 medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: iso stock solution
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: CONTINUOUS
- Light intensity and quality: 4400÷8800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber )
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Annex 4 OECD 215 (excluding end concentrations)
- Range finding study: yes (1- 10- 100 mg/l)
- Test concentrations: 1.5 – 4.6 – 6.8 – 10 – 15 – 22 - 46 mg/l (definitive test)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- zinc heptahydrate suplhate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Flocculation: no
- Aggregation of algal cells: no
- Any stimulation of growth found in any treatment: no - Reported statistics and error estimates:
- The growth rates and the percentages of inhibition were calculated.
The curve dose /effect was done using tese values.
The value of the ErC50 was calculated from the curev dose / effect (calculation made using the Excel software and confirmed with the software R) - Validity criteria fulfilled:
- yes
- Conclusions:
- In a 72h acute toxicty study, the cultures of Pseudokirchneriella subcapitata were exposed to Mercaptamine HCl at measure concentrations of 1.5, 4.6, 6.8, 10, 15, 22, 46 mg/L under static conditions in accordance to OECD guideline 201
The 72h EC50 (calculated form initial measured concentrations) was 19.8 mg/L
This study is classified as reliability 1 and satisfies the guideline requirements for an algal growth inhibition study
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 19.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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