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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial specific surface area
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2019-11-05 to 2019-11-06, with the definitive exposure phase on 2019-11-06 at the test facility
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test item Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol
Batch number ESD0033040
Purity UVBC substance, ca. 95% dry residue
Composition N-Oleyl-DL-methionine: 57 % (w/w) (100 % minus by-products)
By-products:
N-Oleyl-DL-methionine
isopropylester: 24 % (w/w) (gas chromatography, calculated)
Oleic acid: 12.6 % (w/w) (gas chromatography)
Oleic acid
isopropylester: 1.8 (w/w) (gas chromatography)
Methionine: 1.6 % (w/w) (gas chromatography)
Water: 0.5 % (w/w) (Karl Fischer titration)
Unknown
by-products: 2.5 % (a/a) (gas chromatography)
Acid number: 138 mg KOH/g (titration)
Water solubility <10 g/L (25°C) partially
Density 0.98 g/cm3
Appearance Brownish liquid
Expiry date 2021-08-31
Recommended storage Room temperature - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Based on the results of the preliminary test (see Annex I for details) the definitive study was carried out with 5 concentrations within the range of 10 to 1000 mg/L (with and without ATU) in a geometrical series and with a dilution factor of approx. 3.2. No pH adjustment was made.
The test item concentrations were weighed out directly in the test vessels.
Triplicates of the control (without ATU) without test item were included at the beginning and at the end of the replicates without ATU.
Triplicates of the control (with ATU) without test item were included at the beginning of the replicates with ATU and at the end of the test.
The reference item Copper (II)-sulfate was tested with three concentrations within the concentration range 58 - 180 mg/L and a dilution factor of approx. 1.8 (without ATU).
The reference item N-Methylanilin was tested with three concentrations within the concentration range 0.1 – 1.8 – 32 mg/L and a dilution factor of 18 (with and without ATU).
A fresh sample of activated sludge taken from the sewage plant Pattensen was used for the test procedure. It was washed twice and adjusted with chlorine free tap water if necessary to a dry sludge concentration of 3.0 g/L ± 10 %. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Municipal sewage treatment plant of 30982 Pattensen, Germany
Non-adapted activated sludge from the sewage plant at Pattensen is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
- Receipt: 2019-11-05
- Pretreatment: The sludge was washed twice and adjusted with chlorine free tap water if necessary. The sludge was used within 24 h after sampling.
- Dry sludge concentration: 2.89 g/L, corresponding to 1.45 g/L in the test vessels for replicates without ATU and 2.85 g/L, corresponding to 1.43 g/L in the test vessels for replicates with ATU. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not determined
- Test temperature:
- 20.6 °C
- pH:
- pH-value of the activated sludge for test without ATU 7.29
pH-value of the activated sludge for test with ATU 7.25
pH-value of the synthetic waste water 7.21 - Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5
- 500 mL Erlenmeyer flasks, DIN 12380
- Aeration:Permanent, to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension.
- Pretreatment: Stirring overnight in 200 mL ultrapure water at 400 rpm
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6 (Triplicates at the beginning of the test + triplicates at the end of the test.)
- No. of vessels per reference item Copper-II-Sulfat (replicates): 9 (three concentrations within the range 58 - 180 mg/L and a dilution factor 1.8)
- No. of vessels per reference item N-Methylaniline (replicates): 9 (three concentrations within the range 0.1 - 32 mg/L and a dilution factor 18)
- Composition of test medium:
Test concentrations [mg/L] 10 - 32 - 100 - 320 - 1000 mg/L
The test item will be weighed out directly in test vessels
Dilution Table for the Test Item without ATU
Test item concentration [mg/L] 10 32 100 320 1000
Test item [mg] 5 16 50 160 500
Synthetic waste water [mL] 16
Ultrapure water [mL] Filled up to 250 mL
Inoculum [mL] 250
Dilution Table for the Test Item with ATU
Test item concentration [mg/L] 10 32 100 320 1000
Test item [mg] 5 16 50 160 500
Synthetic waste water [mL] 16
ATU solution [mL]* 2.5
Ultrapure water [mL] Filled up to 250 mL
Inoculum [mL] 250
*) Concentration of the ATU solution: 2.32 g/L
The reference item concentration (total respiration) was diluted out of a stock solution.
Dilution Table for the Reference Item (Total Respiration)
Reference item concentration [mg/L] 58 100 180
Stock solution [µL]* 96.7 166.7 300
Synthetic waste water [mL] 16
Demineralized water [mL] Filled up to 250 mL
Inoculum [mL] 250
*) Concentration stock solution: 300 g/L
The reference item concentration (nitrification) was diluted out of a stock solution.
Dilution Table for the Reference Item (Nitrification) without ATU
Reference item concentration [mg/L] 0.1 1.8 32
Stock solution [mL]* 0.05 0.9 16
Synthetic waste water [mL] 16
Demineralized water [mL] Filled up to 250 mL
Inoculum [mL] 250
*) Concentration stock solution: 1 g/L
Dilution Table for the Reference Item (Nitrification) with ATU
Reference item concentration [mg/L] 0.1 1.8 32
Stock solution [mL]* 0.05 0.9 16
Synthetic waste water [mL] 16
ATU solution [mL]** 2.5
Demineralized water [mL] Filled up to 250 mL
Inoculum [mL] 250
*) Concentration stock solution: 1 g/L
**) Concentration of the ATU solution: 2.32 g/L
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter every 5 seconds after transferring the solution to the measuring chamber and the oxygen depletion was recorded for at least 5 minutes with the software PreSens Measurement Studio 2.
TEST CONCENTRATIONS
Inhibitions in the Preliminary Tests
A preliminary range finding test (non GLP) was carried out with the test item concentrations of 1 – 10 – 100 – 1000 mg/L with one replicate each. The pH was measured in one replicate with 1000 mg/L test item. The flasks were aerated for 3 hours.
In the preliminary range finding test, additional replicates were prepared to determine the inhibition of nitrification. One replicate each of 1 – 10 – 100 - 1000 mg/L was measured with the addition of Allylthiourea (ATU).
Inhibitions in the Preliminary Test (with / without ATU)
Nominal Test Item Concentration
[mg/L] pH
Inhibition of total respiration (without ATU)
[%] Inhibition of heterotrophic respiration (with ATU)
[%] Inhibition of nitrification
[%]
1 - 0 1 -2
10 - 5 -3 12
100 - -3 -13 7
1000 6.61 49 48 50
An inhibition of heterotrophic respiration for test item concentration of 1000 mg/L as well as a clear difference between inhibition of nitrification and heterotrophic respiration for test item concentration of 10 mg/L was observed. Due to the request of the sponsor the test was performed with the determination of both endpoints.
A second preliminary range finding test was carried out in the concentrations of 1 - 100 - 1000 mg/L with and without Allylthiourea (ATU) and one replicate
each. Stirring overnight and pH-adjustment were carried out for preparation of the test item solutions. The flasks were aerated for 3 hours.
pH-adjustment in the second Preliminary Test (with / without ATU)
Nominal Test Item Concentration
[mg/L] pH
(test item in ultrapure water) pH
(adjusted with 1 mol/L HCL to) pH
(test item in medium)
1 8.82 - 7.40
100 9.27 7.40 7.44
1000 12.27 7.48 7.42
1 ATU 7.45 - 7.75
100 ATU 11.39 8.88 7.77
1000 ATU 12.28 6.92 7.79
An inhibition of heterotrophic respiration for test item concentration of 1000 mg/L as well as a clear difference between inhibition of nitrification and heterotrophic respiration for test item concentration of 10 mg/L was observed. Due to the request of the sponsor the test was performed with the determination of both endpoints.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 251 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: CI (184 - 359 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 286 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks on result:
- other: CI (158 - 462 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 213 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Remarks:
- respiration rate
- Remarks on result:
- other: CI (118 -423 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 497 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration tate
- Remarks on result:
- other: CI (348 - 697 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 460 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: CI (323 - 776 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 470 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Remarks:
- respiration rate
- Remarks on result:
- other: CI (247 - > 1000 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Reported statistics and error estimates:
- The EC-values of the test item and the reference item were calculated by sigmoidal dose-response regression (biphasic regression where necessary) and linear regression, respectively, using software GraphPadPrism. Calculation of the confidence intervals for the EC50-value was carried out using standard procedures. The coefficient of variation of the oxygen uptake rates of the control replicates was calculated with standard methods using Excel.
The calculation of the oxygen uptake rates (R) and inhibition of respiration rates (I) was performed according to the guideline.
The NOEC was determined by calculation of statistical significance of the oxygen uptake rate in comparison to the control. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC calculation. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-value for both tests was 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is =0.05. - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol for Total Respiration is 100 mg/L.
The EC10 for Total Respiration is 251 mg/L and the EC50 is >1000 mg/L.
The NOEC for heterotrophic respiration is 100 mg/L.
The EC10 for heterotrophic respiration is 286 mg/L and the EC50 is >1000 mg/L.
The NOEC for nitrification is 320 mg/L.
The EC10 for nitrification is 213 mg/L and the EC50 is >1000 mg/L. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol (batch number: ESD0033040) from 2019-11-05 to 2019-11-06, with the definitive exposure phase on 2019-11-06 at the test facility. The test system was activated sludge of the municipal treatment plant of 30982 Pattensen, Germany. The test was carried out under static conditions with the test item concentrations 10 - 32 - 100 - 320 - 1000 mg/L with and without ATU. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of total respiration for the test item replicates ranged from -10 % to 29 %. The mean inhibition of the heterotrophic respiration for the test item replicates ranged from -9 % to 30 %. The mean inhibition of nitrification for the test item replicates ranged from -13 % to 29 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate and N-Methylaniline (with and without ATU) as reference item and the reference toxicity was determined. The EC50-value for the reference item copper (II) sulphate pentahydrate was 104 mg/L. The EC50-value for the reference item N-Methylaniline was
1.92 mg/L.
Endpoints of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol for Inhibition of Respiration
Total Respiration
Heterotrophic Respiration
Nitrification
EC-values
[mg/L]CI
P = 95 [%]
[mg/L]EC-values
[mg/L]CI
P = 95 [%]
[mg/L]EC-values
[mg/L]CI
P = 95 [%]
[mg/L]NOEC
100
–
100
–
320
–
EC10
251
184 – 359
286
158 – 462
213
118 – 423
EC20
497
348 – 697
460
323 – 776
470
247 – > 1000
EC50
> 1000
–
> 1000
–
> 1000
–
EC80
> 1000
–
> 1000
–
> 1000
–
Reference
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L/h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g/h. The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison to the oxygen uptake rates of the control. The results are listed in the tables below.
Oxygen Uptake Rates and Specific Respiration Rates of the Total Respiration of the Control
Repl. Oxygen
Concentration* Oxygen Uptake Rate R Specific Respiration Rate Rs
[mg O2/L] [mg O2/(L×h)] [mg O2/g×h]
Control 1 8.08 23.9 16.5
2 7.92 24.6 17.0
3 7.75 24.3 16.8
4 7.53 21.7 15.0
5 7.32 22.9 15.8
6 7.76 22.4 15.4
Mean value 23.3 16.1
Standard deviation 1.0
CV [%] 4.5
*) determined at start of the measurement
Repl. = Replicate ; CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of the Total Respiration of the
Test Item Concentrations
Test Item Concentration Repl. Oxygen
Concentration* Oxygen Uptake Rate R Inhibition Mean Inhibition
[mg/L] [mg O2/L] [mg O2/(L×h)] [%] [%]
10 1 7.89 22.8 2 -9
2 7.67 27.0 -16
3 7.81 24.2 -4
4 7.60 26.5 -14
5 7.37 26.5 -14
32 1 6.39 25.3 -9 -10
2 6.19 25.8 -11
3 5.95 25.1 -8
4 6.14 26.2 -12
5 5.90 26.1 -12
100 1 7.03 21.5 8 0
2 6.28 25.2 -8
3 6.37 22.8 2
4 7.07 22.2 5
5 7.53 24.4 -5
320 1 6.49 19.7 15 13
2 6.20 21.5 8
3 6.31 20.0 14
4 6.77 20.4 12
5 5.24 19.5 16
1000 1 7.66 17.0 27 29
2 6.94 16.9 27
3 7.51 17.9 23
4 7.88 16.1 31
5 7.48 14.7 37
*) determined at start of the measurement
Repl. = Replicate
Oxygen Uptake Rates of the Heterotrophic Respiration of the Control Replicates with ATU
Repl. Oxygen
Concentration* Oxygen Uptake Rate R
[mg O2/L] [mg O2/(L×h)]
Control 1 8.39 10.5
2 8.27 10.3
3 8.37 10.5
4 7.98 10.2
5 8.30 10.4
6 8.51 10.5
Mean value 10.4
Standard deviation 0.1
CV [%] 1.1
*) determined at start of the measurement
Repl. = Replicate
CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of the Heterotrophic Respiration of the
Test Item Concentrations
Test Item
Concentration
[mg/L] Repl. Oxygen
Concentration*
[mg O2/L] Oxygen Uptake Rate R
[mg O2/Lh] Inhibition
[%] Mean Inhibition
[%]
10 + ATU 1 8.28 10.5 -1 -5
2 8.26 11.1 -7
3 8.35 10.8 -4
4# 7.57 21.6 -108
5 8.26 11.1 -7
32 + ATU 1 8.05 11.5 -11 -9
2 7.80 11.8 -13
3 7.32 12.1 -16
4 7.82 12.0 -15
5 6.86 9.2 12
100 + ATU 1 7.81 11.6 -12 -4
2 7.76 10.2 2
3 7.84 9.6 8
4 7.83 12.5 -20
5 7.91 10.1 3
320 + ATU 1 7.59 8.9 14 12
2 6.84 8.9 14
3 6.69 9.7 7
4 8.23 9.2 12
5 7.56 8.8 15
1000 + ATU 1 7.99 7.3 30 30
2 7.89 7.2 31
3 7.58 7.8 25
4 8.47 7.3 30
5 8.19 7.1 32
*) determined at start of the measurement
#) Replicate without ATU, determined as an outlier (Grubb´s outlier test)
Repl. = Replicate
Calculated Oxygen Uptake Rates of the Nitrification of the Control
Repl. Oxygen Uptake Rate R
[mg O2/(L×h)]
Control 1 13.4
2 14.3
3 13.8
4 11.5
5 12.5
6 11.9
Mean value 12.9
Standard deviation 1.0
CV [%] 7.8
Repl. = Replicate
CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of the Nitrification of the Test Item Concentrations
Test Item
Concentration
[mg/L] Repl. Oxygen Uptake Rate R
[mg O2/Lh] Inhibition
[%] Mean Inhibition
[%]
10 1 11.9 8 -13
2 16.1 -25
3 13.3 -3
4 15.6 -21
5 15.6 -21
32 1 14.0 -8 -11
2 14.5 -12
3 13.8 -7
4 14.9 -15
5 14.8 -15
100 1 10.7 17 4
2 14.4 -12
3 12.0 7
4 11.4 12
5 13.6 -5
320 1 10.6 18 14
2 12.4 4
3 10.9 16
4 11.3 12
5 10.4 19
1000 1 9.7 25 29
2 9.6 26
3 10.6 18
4 8.8 32
5 7.4 43
Repl. = Replicate
Description of key information
The NOEC of Reaction products of DL-methionine and C18 unsaturated fatty acid chloride and isopropanol for Total Respiration is 100 mg/L.
The EC10 for Total Respiration is 251 mg/L and the EC50 is >1000 mg/L.
The NOEC for heterotrophic respiration is 100 mg/L.
The EC10 for heterotrophic respiration is 286 mg/L and the EC50 is >1000 mg/L.
The NOEC for nitrification is 320 mg/L.
The EC10 for nitrification is 213 mg/L and the EC50 is >1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 251 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.