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EC number: 942-225-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From FEB 2003 to MAR 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- For the preparation of the stock solution the test substance was not treated in an ultrasonic bath, because after short mixing in demiralized water a clear solution was obtained (minor deviation)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- For the preparation of the stock solution the test substance was not treated in an ultrasonic bath, because after short mixing in demiralized water a clear solution was obtained (minor deviation)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Schering AG, Bergkamen, Germany - Batch No. 61703003
- Purity test date: 99.5%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Stability: Stable according to reanalysis no. 04205349
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: The substance was dissolved in demineralized water
- Nominal concentration of the unfiltered solution: 125 mg/L
OTHER SPECIFICS:
Selected physio-chemical properties
Water solubility: S20-c > 546 g/L (according to Schering report A09525)
Vapor pressure: P25 < 10-3 Pa (according to Schering report A07301)
Hydrolytic stability: t½ > 1 year at 25°C (according to Schering report A0S 129 - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Remarks:
- dernineralized water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the preparation of the test solutions 125 mg/L iotroxic acid, disodium salt, was dissolved in demineralized water. This solution was used for the preparation of the test solutions.
- Controls: A control solution was prepared with demineralized water without test material.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Demineralized water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): The highest concentration was a 1: 1.25 dilution obtained by adding nutrient solution and inoculum, the further concentrations were obtained by dilutions of 1 :2.5, 1 :5, 1: 10, 1 :20, and 1 :40 with demineralized water, nutrient solution and inoculum. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Sammlung von Algenkulturen, Universitat Goettingen" (Collection of Algae Cultures, University Goettingen)
- Age of inoculum (at test initiation): The algae for the pre-culture were taken from a stock up to seven days old, approximately 72 hours before the start of the incubation in the test.
ACCLIMATION
- Acclimation period: The test vessels were incubated under intermittent stirring for approximately 72 hours in an incubating apparatus (Abimed AlgenTest XT) with continuous light (at approximately 6000-8000 lux) and controlled temperature (water bath). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- The algae were microscopically examined at the end of exposure. The algae of one test vessel of each test concentration group including the control were examined with regard to cell abnormalities
- Test temperature:
- The temperature was 23°C during the whole test period fulfilling the validity criterion.
- pH:
- The pH at the end of the test was between 10.1 and 10.3.
- Nominal and measured concentrations:
- Nominal concentration of iotroxic acid, disodium salt (mg/L): 0.0, 3.1, 6.3, 12.5, 25.0, 50.0, 100.0.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Fluorescence determination: As a parameter for the cell density, the chlorophyll fluorescence in the test and control solutions were determined at 0, 24, 48 and 72 hours of incubation. All measurements were made by means of a fluorescence photometer at a wavelength of 320 and 800 nm for agitation and measurement, respectively. Sampling volume and sampling time was electronically controlled. For statistical reasons, it is aimed that the coefficient of variation between replicates at 72 hours does not exceed 25%. The test is valid, when the fluorescence increase is at least 16-fold in the controls after 72 hours.
Calculation of results and statistical analysis: The values for the average area below the growth curves, specific growth rate, and the percentage inhibition were calculated by the computer program Abimed "AlgenTest XT 2.21 ". The program calculates the respective parameters according to the mathematical formulas given in the OECD guideline (1). The EbCso/72 hours (biomass) and the ErCso/72 hours (growth rate) as well as the 95% confidence limits are also calculated by the above referenced computer program on the basis of a probit analysis. - Validity criteria fulfilled:
- yes
- Remarks:
- The validity criteria recommended by the guideline (1 ), i.e. ≥ 16-fold increase of algae cells after 72 hours in the controls, were fulfilled.
- Conclusions:
- Iotroxic acid, disodium salt had no inhibitory effect on the growth of Desmodesmus subspicatus. The EbC50 (biomass) and the ErC50 (growth rate) was > 100 mg/L.
- Executive summary:
In a 72 hour acute toxicity study, the freshwater aquatic vascular plants green algae Desmodesmus subspicatus were exposed to iotroxic acid, disodium salt at nominal concentrations of 0, 3.1, 6.3, 12.5, 25.0, 50.0, 100.0 mg a.i. /L under static or renewal conditions in accordance with the OECD 201 guideline. The 72 hour ErC50 and EbC50 values based on growth rate and biomass were > 100 and > 100 mg a.i./L, respectively. The validity criteria recommended by the the OECD 201 guideline (i.e. > 16-fold increase of algae cells after 72 hour) in the controls, were fulfilled.
No changes in morphological structures or other abnormalities were observed except some oversized cells in the highest test concentration.
This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 201 Growth inhibition test of iotroxic acid, disodium salt on the green algae Desmodesmus subspicatus toxicity study.
Results Synopsis
Test Organism: Desmodesmus subspicatus
Test Type: Static
72 hour ErC50: >100 mg a.i./L (growth rate)
72 hour EbC50: >100 mg a.i./L (biomass)
Reference
Inhibition of biomass and the growth rate(%) of Desmodesmus suhspicatus after 72 hours exposure to iotroxlc acid, disodium salt |
||
Nominal concentration of iotroxic acid, disodium salt (mg/L) |
Inhibition in percent | |
Biomass (integral) | Growth rate | |
0.0 (control) | 0.0 | 0.0 |
3.1 | -4.6 | 10.4 |
6.3 | -11.0 | 6.3 |
12.5 | -8.5 | -9.4 |
25.0 | -5.6 | -10.7 |
50.0 | -6.4 | -1.1 |
100.0 | -4.0 | -7.2 |
Description of key information
Iotroxic acid, disodium salt had no inhibitory effect on the growth of Desmodesmus subspicatus. The EbC50 (biomass) and the ErC50 (growth rate) was > 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
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