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EC number: 221-334-5 | CAS number: 3069-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 - 29 Apr 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2011
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatlisches Gewerbeaufsichtsamt Hildesheim, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations and method: analytical determination of the test item and the control were carried out from freshly prepared media after 0 h (with algae) and old test media after 72 h (with algae). The hydrolysis product of the test item was analytically verified, since the substance hydrolyses fast in aqueous media.
- Sample storage conditions before analysis: all original samples were stored at room temperature
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of 100 mg/L was prepared in dilution water and was stirred for 24 ± 1 h (1100 rpm, room temperature) - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: HINDAK, SAG 61.81
- Source (laboratory, culture collection): fresh stocks are prepared every month on Z-Agar (algae originaly from Sammlung von Algenkulturen (SAG) Pflanzenphysiologisches Institut der Universitat Goettingen)
- Age of inoculum (at test initiation): a three to four day old preculture, prepared in ditulion water, will be used as inoculum - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.5 - 23.5 °C
- pH:
- in vessels without pH adjustment: 8.05 - 9.67
in vessels with pH adjustment: 8.31 - 9.02
in control vessel: 8.05 - 9.46 - Nominal and measured concentrations:
- 6.25, 12.5, 25, 50, 100, 100 (with pH adjustment) mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile Erlenmeyer flasks (vol. 250 mL) with cotton wool plugs
- Fill volume: 100 mL
- Initial cells density: 5 x 10^3 - 10^4 cells/mL
- Control end cells density: 2088469 cells(mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: pH was measured in one additional replicate of each test item concentration and the control at the start of the exposure. After 24 and 48 h an aliquot of each test item concentration and the control was taken out of the test replicates for pH measurement. The pH measurement at the end of the exposure were conducted in a pooled sample of each test item concentration and the control
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: three replicates of the highest test concentrations, adjusted with 1 M NaOH and HCL to pH 8.1 +/- 0.2
- Photoperiod: 24 h light
- Light intensity and quality: 60 - 120 µE m^-2 s^-1 (fluorescent tubes, OSRAM L 36 W/865, cool daylight)
- Other: oscillation at 70 rpm
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: algae cells were evaluated microscopically at the start and at the end of the incubation period. Ccells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algae cells.
- Chlorophyll measurement: cell density was measured daily via Chlorophyll-a-fluorescence (excitation at 436 nm, emission at 685 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes
- Test concentrations: 10 mg/L and 100 (with pH adjustment and without) mg/L
- Results used to determine the conditions for the definitive study: growth rate inhibition was 6-13% in the 100 mg/L concentrated solution - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
-Observation of abnormalities (for algal test): icroscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities - Results with reference substance (positive control):
- - ErC50: 0.460 mg/L (95% CI: 0.443 - 0.481 mg/L)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study with Pseudokirchneriella subcapitata as the test organism, resulted in a 72 h ErC50 of > 100 mg/L and 72 h NOErC of 50 mg/L. The results are expressed in nominal concentrations, since 100% of the test substance was analytical verified at test end.
Reference
Further biological results reported:
EyC50 > 100 mg/L (nominal)
NOEyC = 50 mg/L (nominal)
Table 1: Cell densities.
Nominal Test Item Concentration [mg/L] |
Mean values of cell densities [cells/mL] | |||
0 hours | 24 hours | 48 hours | 72 hours | |
100 (pH-adjusted) | 6305 | 21188 | 231419 | 1247674 |
100 | 6305 | 12151 | 144951 | 1452935 |
50 | 6305 | 23258 | 295991 | 2064620 |
25 | 6305 | 33666 | 388919 | 2212299 |
12.5 | 6305 | 37933 | 404444 | 2447685 |
6.25 | 6305 | 37335 | 422602 | 2535487 |
Control | 6305 | 34458 | 394313 | 2088469 |
Table 2: Mean growth rate and % inhibition of growth rate.
Nominal Test Item Concentration [mg/L] |
Mean growth rate [d-1] | Inhibition of growth rate [%] |
100 (pH-adjusted) | (+) 1.76 | 9 |
100 | (+) 1.81 | 6 |
50 | (-) 1.93 | 0 |
25 | (-) 1.95 | -1 |
12.5 | (+)* 1.99 | -3 |
6.25 | (+)* 2 | -3 |
Control | 1.93 | - |
(+) Statistically significant differences of growth rates and yield compared to control values
(-) Not significant differences
* Statistically significance caused by growth stimulation
Table 3: Section-by-section and average specific growth rates of the control group (0 - 72 hours).
Replicate No. | Specific growth rate [d-1] section-by-section | Mean {0-72 hours) | SD ± | CV [%] |
Mean CV [%] |
|||
0 - 24 hours | 24-48 hours | 48 - 72 hours | ||||||
Control | 1 | 1.43 | 2.5 | 1.81 | 1.91 | 0.542 | 28.4 | |
2 | 1.87 | 2.35 | 1.66 | 1.96 | 0.358 | 18.3 | ||
3 | 1.85 | 2.39 | 1.63 | 1.96 | 0.391 | 20 | 23.5 | |
4 | 1.67 | 2.35 | 1.73 | 1.92 | 0.377 | 19.6 | ||
5 | 1.74 | 2.43 | 1.71 | 1.96 | 0.41 | 20.9 | ||
6 | 1.56 | 2.63 | 1.48 | 1.89 | 0.642 | 34 | ||
Mean | 1.93 | |||||||
SD ± | 0.03 |
The measured concentrations of the hydrolysis product of the test item in the fresh media (0 h) were in the range of 113 to 118 % of the nominal values at all tested concentration levels. The measured concentrations in the old media (72 h) were in the range of 109 to 120 % of the nominal values of ail concentrations. Therefore, the nominal concentrations of the test item were used for the calculations.
Description of key information
ErC50 (72 h) > 100 mg/L, NOErC (72 h) = 50 mg/L (nominal, OECD 201, P. subcapitata)
Key value for chemical safety assessment
Additional information
The toxicity of N-methyl-3-(trimethoxysilyl)propylamine (CAS 3069-25-8) to algae was tested according to OECD 201 and GLP standards (Noack Laboratories, 2016). In the 72 h study, the test organism Pseudokirchneriella subcapitata was exposed to nominal test item concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L. The highest test item concentration of 100 mg/L was additionally tested with a pH adjustment to a pH of 8.1 ± 0.2. Since the tested substance hydrolyses rapidly in aqueous media, its hydrolysis product was analytically monitored by LC-MS. 109 – 120% of the hydrolysis product was recovered at test end. The test resulted in an NOEC (72 h) of 50 mg/L and an EC50 (72 h) > 100 mg/L.
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