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Diss Factsheets

Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08-Jan-1985 to 22-Jan-1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohexane-1,2,4-triyltris(ethylene)
EC Number:
220-668-9
EC Name:
Cyclohexane-1,2,4-triyltris(ethylene)
Cas Number:
2855-27-8
Molecular formula:
C12H18
IUPAC Name:
1,2,4-triethenylcyclohexane
Test material form:
other: Liquide
Details on test material:
IUCLID4 Test substance: other TS: 1,2,4-Trivinylcyclohexan; purity: 99.5 %

Test animals

Species:
rabbit
Strain:
other: Kleine weiße Russen, Chbb-SPF
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Species/Strain: Rabbit (Kleine weiße Russen), Chbb-SPF
- Source: Fa. Dr. Karl Thomae GmbH, Biberach
- Weight at study initiation: 2.2 - 2.5 kg
- Housing: single housing
- Diet: K4 Alleindiät für Kaninchen, SSniff Spezialfutter GmbH, 4770 Soest, ad libitum
- Water: Tap water, ad libitum
- Acclimation period: 14 days befor the first test-substance application

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/-1°C
- Humidity (%): 60 +/-5 %
- Air changes (per hr): 15fold
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Amount / concentration applied:
0.5 ml, undiluted
Duration of treatment / exposure:
4 hour(s)
Observation period:
14 days
Number of animals:
3 Males and 3 Females
Details on study design:
Application of 0.5 ml of undiluted test substance on a 6 cm2 shaved area of skin covered with a gauze patch. Removal of residual test substance after 4 hours using water. Evaluation of results 1 and 24 hours after removal of the patch, and after 6, 8, 10, 14 days.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
animal #4
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
animal #5
Time point:
24/48/72 h
Score:
2.3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
animal #6
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
other: Trend to reversibility clearly conceivable at the end of the observation period of 14 days.
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
2.3
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #4
Time point:
24/48/72 h
Score:
1.7
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #5
Time point:
24/48/72 h
Score:
1.3
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #6
Time point:
24/48/72 h
Score:
1.3
Max. score:
4
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
The test substance is irritating to skin.

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The test substance is irritating to skin.