Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-668-9 | CAS number: 2855-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- EC 84/449, Nov 1989
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A suspension of 1 g/L of the test substance in synthetic freshwater was stirred for 18 hours. Then the solution was filtered and the carbon content was measured. This solution has been used as stock solution. The TOC in the stock solution was 15.8 mg/L corresponding to 17.1 mg/L test substance. The calculated test substance concentrations of 3.8 mg/l, 5.5. mg/L, 7.7 mg/L, 11.1 mg/L, and 15.4 mg/L were used in the test.
- Controls: freshwater without test substance
- Evidence of undissolved materia: not specified - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus, Strain IRCHA
- Source: breeding in the laboratory (Klon 5)
- No feeding during the test
ACCLIMATION
- Acclimation conditions (same as test or not): Breeding in M4-medium according to Elendt in 1L vessels
- Type and amount of food: Scenedesmus suspicatus, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): parthenogenetic reproduction is warranted
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Adult daphnids were isolated 24 h before the test begin. The offspring has been transfered into test water und has been used for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- pH:
- 8.0 - 8.6 at test end
- Dissolved oxygen:
- 8.7 - 8.8 mg O2/l at test end
- Nominal and measured concentrations:
- concentration of the stock solution 17.1 mg/L. Test concentrations 3.8 mg/L; 5.5 mg/L; 7.7 mg/L; 11.1 mg/L; 15.4 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Test vessel with 10 mL test solution
- Aeration: no
- No. of organisms per vessel: 5 daphnids per vessel (1 daphnid/ 2 mL)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Source/preparation of dilution water: synthetic freshwater
- Photoperiod: darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : (im)mobility
VEHICLE CONTROL PERFORMED: no vehicle - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 15.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 15.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 15.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The highest test substance concentration, where no immobility has been observed was 15.4 mg/L after 48 h. The lowest concentration with 100% immobility after 48 h is >15.4 mg/L. The EC50 is >15.4 mg/L after 48 h exposure. This corresponds to the highest test substance concentration tested and is the limit of water solubility found in the test. All concentrations are based on the product.
- Results with reference substance (positive control):
- A concentration of 0.9 mg/L potassium dichromate resulted in 5 % immobilised daphnids after 24 h. 1.9 mg/L potassium dichromate showed 95% immobilised daphnids after 24 h exposure.
- Reported statistics and error estimates:
- An EC50 value could not be determined by probit analysis referring to Cavalli-Sforza (1972).
- Validity criteria fulfilled:
- yes
- Remarks:
- Not more than 10% of the daphnids in the control are immobilised. The dissolved oxygen concentration is >3 mg/L at the test end.
- Conclusions:
- In a static toxicity test according to EC 84/449/EWG part C2 and OECD202 (1984), the acute toxicity of 1,2,4-Trivinylcyclohexane on Daphnia magna was investigated. The highest tested concentration at which no immobilisation (<=10%) was 15.4 mg/L. The EC50 after 48 h is >15.4 mg/L. The concentration of 15.4 mg/L is the highest test substance concentration tested and is the limit of water solubility found in the test. The lowest concentration with 100% immobilisation is >15.4 mg/L.
Reference
Description of key information
The acute toxicity on Daphnia magna of 1,2,4 -Trivinylcyclohexane has been examined according to EC 84/449/EWG part C2 and OECD202 (1984). The EC50 for daphnia is >15.4 mg/L after 48 h.
Key value for chemical safety assessment
Additional information
The acute toxicity of 1,2,4 -Trivinylcyclohexane on Daphnia magna has been examined in a GLP conform toxicity test according to EC 84/449/EWG part C2 and OECD202 (1984). The highest tested concentration without any observed immobilisation (<=10%) has been 15.4 mg/L, which is the highest tested test substance concentration and displays the limit of water solubility found in the test. Therefore, the EC50 for daphnia is >15.4 mg/L after 48 h of exposure.
In this study, the test substance concentration has not been measured by a substance-specific analytical method. Nevertheless, the study is a guideline study, which has been performed in accordance to GLP, and the results are sufficiently documented.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.