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EC number: 219-135-3 | CAS number: 2370-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-08-27 to 2012-08-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17 July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.25, 12.5, 25.0, 50.0, 100 mg/L
freshly prepared media: after 0 and 72 h, 24 hold test media: after 24 and 96 h
- Sample storage conditions before analysis: All original sampies were stored at 6 ± 2 °C until sample preparation, if necessary. Prepared samples were stored at room temperature until analysis. - Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Niedersächsischer Landesbetrieb für Wasserwirtschaft, Küsten- und Naturschutz, An der Scharlake 39, 31135 Hildesheim, Germany
- Length at study initiation (length definition, mean, range and SD): 2.4 cm
- Weight at study initiation (mean and range, SD): 0.243 g
- Feeding during test: no
ACCLIMATION
- Acclimation period: at least 12 d
- Type and amount of food: Sera Vipan; SERA GMBH; 4% of body weight per feeding day; fish were not fed 24 h before the test started
- Feeding frequency: 3 times/week
- Health during acclimation (any mortality observed): only fish with mortality <5% were used for the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 48 mg CaCO3/L
- Test temperature:
- 23.5 ± 0.2 °C
- pH:
- 7.47-7.84
- Dissolved oxygen:
- 82-100% saturation
- Nominal and measured concentrations:
- nominal 6.25, 12.5, 25.0, 50.0, 100 mg/L;
measured concentrations in freshly prepared media were in the range of 94 - 98 % of the nominal values and 90 - 95 % in 24 h aged test media - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria of 14 L, filled with 10 L of dilution water
- Type: covered with a floating lid (aluminium foil) to reduce the loss of the test item via the surface
- Aeration: no
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: fish density in the tanks was 0.170 g fish per liter test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water of local origin, filtered on activated charcoal and aerated for at least 24 h to remove chlorine
OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: natural
- Light intensity: 2.59 to 3.03 µmol photons/m²/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality (considered dead if there was no visible movement): 24, 48, 72, 96 h
Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 100% mortality at 100 mg/L - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 52.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 48.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - 7/7 fish were dead after 24 h in the 100 mg/L group, 3/7 fish died in the 50 mg/L group, no mortality up to 25 mg/L
- Behavioural abnormalities: lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities up to 25 mg/L
- Mortality of control: 0/7 fish died - Results with reference substance (positive control):
- n/a
- Sublethal observations / clinical signs:
Cumulative mortality
Nominal concentration [mg/L]
Cumulative mortality [%]
24 h
48 h
72 h
96 h
100
100
100
100
100
50
0
14
43
71
25
0
0
0
0
12.5
0
0
0
0
6.25
0
0
0
0
0 (control)
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 of ETMA in Danio rerio (zebrafish) was 48.7 mg a.i./L.
- Executive summary:
In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.
The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).
Sublethal effects like lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities were observed up to a concentration of 25 mg/L.
Results Synopsis
Test organism size/age: Danio rerio, mean length 2.4 cm
Test Type: Static Renewal
LC50: 48.7 mg a.i./L
Endpoint(s) Effected: mortality
Reference
Description of key information
96 h LC50 = 48.7 mg/L; OECD guideline 203; GLP
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 48.7 mg/L
Additional information
One short-term study in freshwater fish is available for ETMA, which is considered reliable without restriction (Klimisch score 1).
In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.
Sublethal effects like lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities were observed up to a concentration of 25 mg/L. The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).
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