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EC number: 915-640-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to guideline and/or standard method but was non-GLP.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: EPA, 1972. Fish - pesticide acute toxicity test guideline. Pesticides Regulation Division, Environmental Protection Agency.
- Deviations:
- no
- Remarks:
- Not specified in report
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data - Analytical monitoring:
- no
- Details on sampling:
- No data
- Vehicle:
- no
- Details on test solutions:
- All test solutions were prepared in water on a weight-volume basis immediately prior to administering.
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- Source: Zetts Fish Hatcheries, Drifting, Pa.
Date: 5-21-73
Average Weight (g) : 0.85 Range (g) : 0.61-1.41
Average Length (mm) : 38.7 Range (mm) : 34-51
Loading (g/l): 0.49
All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- 32 mg/L CaCO3
- Test temperature:
- 75F +/- 2F
- pH:
- 7.2-7.6
- Dissolved oxygen:
- 7.8-8.1 mg/L
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal- 0, 4.60, 4.90, 5.24, 5.60, and 6.50 mg/L
- Details on test conditions:
- Water used in the static test was aerated deionized distilled water with 30 mg/l CaSO4, 30 mg/l MgSO4, 48 mg/l NaHCO3, and 2 mg/l KCL added.
Tests were conducted by placing 16 liters of reconstituted water in each vessel (Glass - 20 liter aquaria), aerating, then adding the fish. Fish loading requirements (<1.0 gm fish / liter water) limited the number of fish to 10 per vessel. If no deaths occurred in 24 hours, aeration was stopped and the test compound added with 1,500 mls of water for mixing, making a total of 17.5 liters. Any vessels containing dead fish were cleaned, reset, and observed for another 24 hours prior to compound addition. Constant temperature water troughs maintained the temperature +/- 2F.
Twenty fish were exposed to each concentration. Observations were made and recorded and dead fish removed daily at the same time for four days (96 hours).
A reference p,p' DDT toxicity was run for both species of fish.
All test solutions were prepared in water on a weight-volume basis immediately prior to administering. - Reference substance (positive control):
- yes
- Remarks:
- p,p' DDT
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.99 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 4.75-5.17
- Details on results:
- Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 4.06 3.44-4.40 5.22 4.98-5.42 6.71 6.26-7.71
48 4.10 3.57-4.40 5.06 4.84-5.23 6.24 5.92-6.88
72 4.09 3.56-4.39 5.03 4.81-5.20 6.19 5.88-6.80
96 4.02 3.45-4.34 4.99 4.75-5.17 6.19 5.88-6.84
"Confidence Limits
Controls- No deaths in controls - Results with reference substance (positive control):
- p,p' DDT
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 3.4 1.8-4.0 5.1 4.5-5.7 7.7 6.5-13.7
48 3.0 1.3-3.7 4.4 3.4-4.9 6.6 5.9-10.4
72 2.4 0.2-3.4 3.9 1.6-4.5 6.3 5.5-13.9
96 2.0 0.0-3.1 3.5 0.02-4.3 6.2 5.3-141.5
"Confidence Limits - Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 at 96 hours for bluegill is 4.99 mg/l.
- Executive summary:
The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.
Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to standard method but was non-GLP.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 96-hour flow-through toxicity test in fathead minnows using dechlorinated Lake Huron water
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data - Analytical monitoring:
- no
- Details on sampling:
- No data
- Vehicle:
- no
- Details on test solutions:
- Dechlorinated Lake Huron water was used for the flow-through toxicity with fathead minnows.
The flow-through system was thoroughly cleaned and rinsed with the appropriate toxicant concentration. The toxicant stock solution was made up in water and placed in a Mariotte bottle immediately prior to the test. The system was activated at least three hours before the fish were added so that equilibrium could be reached. - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Source: White Bear Lake, Minn.
Date: 2-27-73
Average Weight (g) : 1.25 Range (g) : ,0.59-2.00
Average Length (mni): 49 Range (mm) : 43-60
All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- No data
- Test temperature:
- 54F +/- 2F
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal- 0, 2.82, 3.75, 5.00 mg/L
- Details on test conditions:
- Dechlorinated Lake Huron water was used for the flow-through toxicity with fathead minnows. The flow-through system was thoroughly cleaned and rinsed with the appropriate toxicant concentration. The toxicant stock solution was made up in water and placed in a Mariotte bottle immediately prior to the test. The system was activated at least three hours before the fish were added so that equilibrium could be reached.
Ten fathead minnows were placed in each aquarium (glass, 8 liters) with a discharge rate of the diluter being approximately once every eight minutes, or a turnover rate per aquarium of about one/hour. This turnover rate was sufficient to keep ample D.O. A constant temperature water trough maintained the temperature +/-2F. Ten fish were exposed to each concentration. - Reference substance (positive control):
- yes
- Remarks:
- p,p' DDT (acute static)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 3.25-4.11mg/L
- Details on results:
- Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 3.15 1.94-3.64 4.26 3.71-5.25 5.76 4.85-11.04
48 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
72 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
96 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
"Confidence Limits
Controls- one control was injured at 24h and died - Results with reference substance (positive control):
- p,p' DDT (Acute Static)
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 13.87 --- 18.16 --- 23.76 ---
48 12.60 --- 20.32 --- 32.75 ---
72 12.13 0-13.8 17.47 14.88--- 25.14 18.17---
96 10.05 0-11.86 15.89 13.74-e5 25.13 17.92---
"Confidence Limits - Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 at 96 hours for fathead minnow is 3.66 mg/l.
- Executive summary:
The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.
Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to guideline and/or standard method but was non-GLP.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: EPA, 1972. Fish - pesticide acute toxicity test guideline. Pesticides Regulation Division, Environmental Protection Agency.
- Deviations:
- no
- Remarks:
- Not specified in report
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data - Analytical monitoring:
- no
- Details on sampling:
- No data
- Vehicle:
- no
- Details on test solutions:
- All test solutions were prepared in water on a weight-volume basis immediately prior to administering.
- Test organisms (species):
- other: Salmo gairdneri Richardson
- Details on test organisms:
- Source: Platte River, Michigan
Date: 10-1-73
Average Weight (g): 1.19 Range ( g ) : 0.95-1.650
Average Length (mm) : 54 Range (mm): 48-59
Loading (g/l) : 0.68
All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- 32 mg/L CaCO3
- Test temperature:
- 55F +/- 2F
- pH:
- 7.2-7.6
- Dissolved oxygen:
- 7.8-8.1 mg/L
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal- 0, 4.20, 4.60, 5.24, 5.60, 6.50, and 7.50 mg/L
- Details on test conditions:
- Water used in the static test was aerated deionized distilled water with 30 mg/l CaSO4, 30 mg/l MgSO4, 48 mg/l NaHCO3, and 2 mg/l KCL added.
Tests were conducted by placing 16 liters of reconstituted water in each vessel (Glass - 20 liter aquaria), aerating, then adding the fish. Fish loading requirements (<1.0 gm fish / liter water) limited the number of fish to 10 per vessel. If no deaths occurred in 24 hours, aeration was stopped and the test compound added with 1,500 mls of water for mixing, making a total of 17.5 liters. Any vessels containing dead fish were cleaned, reset, and observed for another 24 hours prior to compound addition. Constant temperature water troughs maintained the temperature +/- 2F.
Twenty fish were exposed to each concentration. Observations were made and recorded and dead fish removed daily at the same time for four days (96 hours).
A reference p,p' DDT toxicity was run for both species of fish.
All test solutions were prepared in water on a weight-volume basis immediately prior to administering. - Reference substance (positive control):
- yes
- Remarks:
- p,p' DDT
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 4.72-5.28 mg/L
- Details on results:
- Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 5.16 4.64-5.44 5.95 5.68-6.33 6.85 6.42-7.96
48 4.87 4.28- 5.21 5.80 5.47-6.14 6.90 6.46-7.82
72 4.43 3.84-4.73 5.17 4.88-5.43 6.02 5.68-6.80
96 4.27 3.65-4.58 5.02 4.72-5.28 5.91 5.57- 6.69
"Confidence Limits
Controls- No deaths in controls - Results with reference substance (positive control):
- p,p' DDT
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 9.7 7.2-11.0 13.5 12.1-15.2 18.8 16.4-26.2
48 8.7 6.6-9.9 11.7 10.4-12.8 15.6 14.0-19.6
72 7.6 5.1-9.0 11.0 9.5-12.4 16.1 14.1-21.6
96 5.5 2.3-7.3 9.2 6.7-10.7 15.2 12.9-23.3
"Confidence Limits - Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 at 96 hours for rainbow trout is 5.02 mg/l.
- Executive summary:
The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.
Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.66 mg/L
Additional information
There are multiple short-term studies forDOWFAX™ 3B2 with freshwater fish species: Fathead minnow (Pimephales promelas) 96 h LC50 was 1.65 mg/L (a.i.) or 3.66 mg/L formulation;Rainbow trout (Salmo gairdneri) 96 h LC50 was 2.26 mg/L (a.i.) or5.02 mg/L formulation; Bluegill sunfish (Lepomis macrochirus) 96 h LC50 was 2.25 mg/L (a.i.) or 4.99 mg/L formulation.
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