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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 August 2011 to 26 March 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable. Due to low aqueous solubility of the test substance, it was not possible to develop a suitable method of analysis with sufficient sensitivity to verify concentrations during the study. Therefore, results are reported in terms of the nominal loading rates.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test medium (100 mg/L) was prepared by weighing the test substance (100 mg) onto a glass cover slip, which was then added to a volumetric flask (1 L) and made up to volume with dilution medium. The contents were stirred overnight in the dark before the stirrer was turned off and a siphon tube inserted at the mid-vessel point. The bulk medium was then left to stand overnight, after which the mid-vessel fraction was siphoned off and used in the test (nominal loading rate of 100 mg/L WAF). - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source (laboratory, culture collection): a reputable supplier
- Age of inoculum (at test initiation): three days
- Method of cultivation: The liquid slope cultures were stored in an illuminated refrigerator. Sterile algal nutrient medium was inoculated with cells aseptically removed from the slope culture; these primary liquid cultures (100 mL) were incubated for approximately three days in an orbital incubator under continuous illumination (nominal temperatures 21 to 25C) to provide an inoculum in the log phase of growth, characterised by a cell density of 1.8 x E6 cells/mL.
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Hardness:
- Not reported
- Test temperature:
- 21.9 - 23.4 °C
- pH:
- 7.78 - 9.84
- Dissolved oxygen:
- Not reported
- Salinity:
- Not applicable to a freshwater study.
- Nominal and measured concentrations:
- Nominal concentration = 100 mg/L
Measured concentrations were not determined due to the very low water solubility of the test substance. - Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flask
- Type: loosely plugged with a foam bung
- Material, size, headspace, fill volume: glass, each flask contained 100 mL of control or test culture
- Aeration: orbital shaker, oscillating at a nominal 130 - 150 cycles per minute.
- Renewal rate of test solution (frequency/flow rate): none, test conducted under static conditions
- Initial cells density: 1.16 x E4/mL.
- Control end cells density: 1636117 cells/mL
- Test end cells density: 1916478 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered, dechlorinated tap water which had been softened and treated by reverse osmosis, before microfiltration and purification (resistivity of 18 Megohm/cm).
- Resistivity: 18 Megohm/cm)
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: none
- Photoperiod: continuous
- Light intensity and quality: nominally 4440 to 8880 lux, provided by 6 x 30 W “cool white” 1 metre fluorescent tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
The range finding test employed nominal test concentrations of 1.0, 10 and 100 mg/L. After 72 hours, no inhibition of growth occurred at 1.0, 10 or 100 mg/L. A definitive limit test was therefore carried out at a nominal loading rate of 100 mg/L. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- No microscopic abnormalities of the cells were detected.
The mean coefficient of variation (CoV) for section by section daily growth rates in control cultures was 9.2 during the definitive test and the CoV for the average specific growth rates of control cultures was 2.13 during the 72 hour exposure period.
Due to low aqueous solubility of the test substance, it was not possible to develop a suitable method of analysis with sufficient sensitivity to verify concentrations during the study to determine the acute toxicity of Daphnia Magna (HLS Study number MZD0032). Therefore, test results have been reported in terms of nominal loading rates. - Validity criteria fulfilled:
- yes
- Remarks:
- Specific growth rate of at least 0.92/day. Mean CoV for daily growth rates in controls <=35% and CoV average specific growth rate in controls <=7%.
- Conclusions:
- LZ1554 was not found to be acutely toxic to algae when dispersed in medium at a nominal loading rate of 100 mg/L.
- Executive summary:
The acute algal toxicity was determined in a GLP-compliant study in accordance with OECD no. 201 and EC test method C.3. LZ1554 was not found to be acutely toxic to algae when dispersed in medium at a nominal loading rate of 100 mg/L.
Reference
Description of key information
Algae
LZ1554 was not found to be acutely toxic to algae (Pseudokirchneriella subcapitata) when dispersed in medium at a nominal loading rate of 100 mg/L. Hence, the 72 hour EL50 (biomass, growth rate, yield) as well as the NOELR were determined to be > 100 mg/L nominal.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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