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EC number: 200-665-9 | CAS number: 67-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
AMES test
In the bacterial reverse mutation test using S.typhimurium strains TA98, TA100, TA1535 and TA1538, test substance did not induce any significant increase of His revertants. Thus, it is concluded that test substance is not genotoxic.
In vitro gene mutation assay in Mammalian cells
In the in vitro chromosome aberration test using Chinese Hamster Lung (CHL) cells, no abberation effects were seen.Therefore, the test substance is considered to be non mutagenic in Chinese Hamster Lung (CHL) cells.
Link to relevant study records
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from peer-reviewed journal
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate genetic potential of substance dimethyl sulphone (Methylsulfonylmethane).
- GLP compliance:
- no
- Type of assay:
- in vitro mammalian chromosome aberration test
- Target gene:
- Chinese Hamster Lung (CHL) cells
- Species / strain / cell type:
- mammalian cell line, other: Chinese Hamster Lung (CHL) cells
- Details on mammalian cell type (if applicable):
- Details on mammalian cell line
- Type and identity of media: The cell were cultured in Eagles minimum essential medium (EMEM) supplemented with 10% fetal bovine serum. The cell were incubated in a 95% air and 5% CO₂ atmosphere at 37°C. The cell were plated at a density of 2.5ẋ10⁵ cells on 6cm plates and grown for 22h, and then colcemid (0.25g/ml) was added 2hr before harvest.
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: No data available
- Periodically checked for karyotype stability: No data available
- Periodically "cleansed" against high spontaneous background: No data available - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 fraction
- Test concentrations with justification for top dose:
- 0, 5, 2.5, 1.25 mg/ml
- Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: No data available
- Justification for choice of solvent/vehicle: No data available - Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- methylmethanesulfonate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: plate incorporation
DURATION
- Preincubation period: No data available
- Exposure duration: 24hr
- Expression time (cells in growth medium): 22 hr
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): 24 hr
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: No data available
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: No data available
OTHER EXAMINATIONS
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available - Rationale for test conditions:
- No data
- Evaluation criteria:
- No data
- Statistics:
- No data
- Key result
- Species / strain:
- mammalian cell line, other: Chinese Hamster Lung (CHL) cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data available
- Effects of osmolality: No data available
- Evaporation from medium: No data available
- Water solubility: No data available
- Precipitation: No data available
- Other confounding effects: No data available
RANGE-FINDING/SCREENING STUDIES: No data available
COMPARISON WITH HISTORICAL CONTROL DATA: No data available
ADDITIONAL INFORMATION ON CYTOTOXICITY: No data available - Remarks on result:
- other: no mutagenic potential
- Conclusions:
- In the in vitro chromosome aberration test using Chinese Hamster Lung (CHL) cells, no abberation effects were seen.Therefore, the test substance is considered to be non mutagenic in Chinese Hamster Lung (CHL) cells.
- Executive summary:
The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate the potential of test substance damaging chromosome. The cell were exposed to test substance at concentration 0, 5, 2.5, 1.25 mg/ml for 24 hr. Methylmethane sulfon ate (MMS)(0.02 mg/ml) and Benzo[a]pyrene (B[a]P) (0.02mg/ml) used as positive control substances. After treatment with test substance , no significant increase in the number of aberrant cells was observed. In presence or absence of S9 mix, the chromosome aberration produced by treatment with Methylsulfon ylmethane was less than 2 %. These results indicate that methylsulfonylmethane did not increase chromosome aberration as compared with the negative control. Therefore, the test substance is considered to be non genotoxic.
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from peer-reviewed journal
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The potential genotoxicity of test substance was evaluated in S. typhimurium strains TA98, TA100, TA1535 and TA1538 by Ames test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- No data available
- Target gene:
- Salmonella typhimurium TA98, TA100, TA1535 and TA1538
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535 and TA1538
- Details on mammalian cell type (if applicable):
- No data available
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 2500, 5000, 1000 µg/plate
- Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: No data available
- Justification for choice of solvent/vehicle: No data available - Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 9-aminoacridine hydrochloride (9-AA)
- Remarks:
- For Strain TA1538 (50 µg/plate)
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hr
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: 3 replicate plates per dose
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: No data available
OTHER EXAMINATIONS: No data available
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available - Rationale for test conditions:
- No data available
- Evaluation criteria:
- No data available
- Statistics:
- No data available
- Key result
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data available
- Effects of osmolality: No data available
- Evaporation from medium: No data available
- Water solubility: No data available
- Precipitation: No data available
- Other confounding effects: No data available
RANGE-FINDING/SCREENING STUDIES: No data available
COMPARISON WITH HISTORICAL CONTROL DATA: No data available
ADDITIONAL INFORMATION ON CYTOTOXICITY: No data available - Remarks on result:
- other: no mutagenic potential
- Conclusions:
- In the bacterial reverse mutation test using S.typhimurium strains TA98, TA100, TA1535 and TA1538, test substance did not induce any significant increase of His revertants. Thus, it is concluded that test substance is not genotoxic.
- Executive summary:
The reverse mutation tests were carried out using S. typhimurium strainsTA98, TA100, TA1535 and TA1538. A 0.1 ml volume of the test substance solutions (2500, 5000, 1000 µg/plate) was mixed with 0.1ml of the 10 h cultured bacteria and then bacterial culture was mixed with 0.5ml of the S9 mix or a 0.1 M phosphate buffer (pH 7.4) and incubated for 20min at 37 °C. After incubation a top agar (2ml) was added and the mixture overlaid on a minimal glucose agar plate. After 48 hrs of incubation, the revertant colonies were counted.The treatments were performed in the presence and absence of metabolic activation. There was no increase in the revertant numbers as a result of test substance treatment for any of the S.typhimurium strain with and without the S9 mix. Thus, it was concluded that test substance was non mutagenic in S.typhimurium strains TA98, TA100, TA1535 and TA1538.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
The micronucleus assay results confirmed that dimethyl sulphone (methylsulfonylmethane) at doses up to 5000 mg/kg did not exhibit any genotoxicity in mouse bone marrow.
Link to relevant study records
- Endpoint:
- genetic toxicity in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from peer-reviewed journal
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The in vivo micronucleus test was carried out in mouse bone marrow, to find out genetic effect of dimethyl sulphone (Methylsulfonylmethane).
- GLP compliance:
- no
- Type of assay:
- other: micronucleus assay
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- No data available
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: Carboxymethylcellulose
- Justification for choice of solvent/vehicle: No data available
- Concentration of test material in vehicle: No data available
- Amount of vehicle (if gavage or dermal): 0.5% w/v (vehicle control group)
- Type and concentration of dispersant aid (if powder): No data available
- Lot/batch no. (if required): No data available
- Purity: No data available - Details on exposure:
- No data available
- Duration of treatment / exposure:
- 48 hr
- Frequency of treatment:
- Once
- Post exposure period:
- No data available
- Dose / conc.:
- 1 250 other: mg/kg
- Dose / conc.:
- 2 500 other: mg/kg
- Dose / conc.:
- 5 000 other: mg/kg
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Positive control(s):
- Positive controls: mitomycin C
- Justification for choice of positive control(s): No data available
- Route of administration: intra peritoneal (i.p)
- Doses / concentrations: 4 mg/kg - Tissues and cell types examined:
- bone marrow
- Details of tissue and slide preparation:
- No data available
- Evaluation criteria:
- No data available
- Statistics:
- No data available
- Sex:
- not specified
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: non mutagenic
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: No data available
- Solubility: No data available
- Clinical signs of toxicity in test animals: No data available
- Evidence of cytotoxicity in tissue analyzed: No data available
- Rationale for exposure: No data available
- Harvest times: No data available
- High dose with and without activation: No data available
- Other:
RESULTS OF DEFINITIVE STUDY
- Types of structural aberrations for significant dose levels (for Cytogenetic or SCE assay): No data available
- Induction of micronuclei (for Micronucleus assay): No data available
- Ratio of PCE/NCE (for Micronucleus assay): No data available
- Appropriateness of dose levels and route: No data available
- Statistical evaluation: No data available - Conclusions:
- The micronucleus assay results confirmed that test substance at doses up to 5000 mg/kg did not exhibit any genotoxicity in mouse bone marrow.
- Executive summary:
The in vivo micronucleus test was carried out in mouse bone marrow, to find out genetic effect of test substance . Methylsulfonylmethane was administered to group of mice using an oral gavage at doses of 1250, 2500, 5000mg/kg. The vehicle control groups received carboxymethyl cellulose at an equivalent oral volume of 0.5% w/v, whereas the positive control group received an i.p. dose of mitomycin C at 4mg/kg. No significant difference in body weight was noted between test substance treated mice and the solvent control. The number of micronucleated polychromatic erythrocytes, where the frequency in the solvent control was 0.4±0.5, the frequency in the positive control was 12.2±1.2, which was significantly higher and the frequency after test substance treatment with 1250, 2500, 5000mg/kg was 0.1±0.5, 0.5±0.5 and 0.4±0.5 respectively. Therefore, the test substance treatment did not cause any change in the frequency of micronucleated polychromatic erythrocytes when compared with the solvent control. Consequently, the micronucleus assay results confirmed that test substance at doses up to 5000 mg/kg did not exhibit any genotoxicity in mouse bone marrow.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Gene mutation in vitro:
Data for the various test chemicals was reviewed to determine the mutagenic nature of dimethyl sulfone, methanesulfonylmethane (67-71-0). The studies are as mentioned below:
AMES test
The reverse mutation tests were carried out using S. typhimurium strainsTA98, TA100, TA1535 and TA1538. A 0.1 ml volume of the test substance solutions (2500, 5000, 1000 µg/plate) was mixed with 0.1ml of the 10 h cultured bacteria and then bacterial culture was mixed with 0.5ml of the S9 mix or a 0.1 M phosphate buffer (pH 7.4) and incubated for 20min at 37 °C. After incubation a top agar (2ml) was added and the mixture overlaid on a minimal glucose agar plate. After 48 hrs of incubation, the revertant colonies were counted.The treatments were performed in the presence and absence of metabolic activation. There was no increase in the revertant numbers as a result of test substance treatment for any of the S.typhimurium strain with and without the S9 mix. Thus, it was concluded that test substance was non mutagenic in S.typhimurium strains TA98, TA100, TA1535 and TA1538.
Supported by other studies.
The mutagenic potential of test substance was insvestigated in a reverse mutation assay in Salmonella typhimurtum strains TA98, TA100, TA102, TA1535 and TA1537 in the presence and absence of S9 liver homogenate fraction. Two independent experiments were conducted. The first trial was conducted using the plate incorporation assay with and without metabolic activation, while the second one was conducted without metabolic activation and the preincubation method with metabolic activation. In both experiments, five concentrations of MSM ranging from 50 to 5000 ug/plate were tested. No significant cytotoxic effects of MSM were noted. test substance did not induce any significant increase in the number of reversions, either in the presence or absence of metabolic activation.Therefore,test substance did not meet the criteria for a potential mutagen, at concentrations up to 5000 µg/plate. Thus, it was concluded that test substance was non mutagenic at concentrations up to 5000 µg/plate in S.typhimurium strains TA98, TA100, TA102, TA1535 and TA1537 with and without the metabolic activation.
Pre-incubation Ames test was carried out according to the method of Maron and Ames (1983), to observe genetic effect of test substance in S.typhimurium strains TA98, TA100 and TA102. All strains were tested at dose 0.003-300 µmol/plate both with and without metabolic activation (RAT, LIVER, S-9, AROCLOR 1254). Four plates were used per dose and incubated for 3 days and the colonies counted with an automated Fisher Count-All colony counter. 2-nitrofluorene, sodium azide, mitomycin C, 2-aminofluorene used as a positive control substances. Water is used as solvent as well as negative control. In results,test substance dose not induced any mutagenic activity in any of the tester strains. Therefore, the test substance is considered to be non mutagenic in Salmonella typhimurium strains TA98, TA100 and TA102 with and without metabolic activation.
In vitro gene mutation assay in Mammalian cells
The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate the potential of test substance damaging chromosome. The cell were exposed to test substance at concentration 0, 5, 2.5, 1.25 mg/ml for 24 hr. Methylmethane sulfon ate (MMS)(0.02 mg/ml) and Benzo[a]pyrene (B[a]P) (0.02mg/ml) used as positive control substances. After treatment with test substance , no significant increase in the number of aberrant cells was observed. In presence or absence of S9 mix, the chromosome aberration produced by treatment with Methylsulfon ylmethane was less than 2 %. These results indicate that methylsulfonylmethane did not increase chromosome aberration as compared with the negative control. Therefore, the test substance is considered to be non genotoxic.
Genetic toxicity in vivo
The in vivo micronucleus test was carried out in mouse bone marrow, to find out genetic effect of test substance . Methylsulfonylmethane was administered to group of mice using an oral gavage at doses of 1250, 2500, 5000mg/kg. The vehicle control groups received carboxymethyl cellulose at an equivalent oral volume of 0.5% w/v, whereas the positive control group received an i.p. dose of mitomycin C at 4mg/kg. No significant difference in body weight was noted between test substance treated mice and the solvent control. The number of micronucleated polychromatic erythrocytes, where the frequency in the solvent control was 0.4±0.5, the frequency in the positive control was 12.2±1.2, which was significantly higher and the frequency after test substance treatment with 1250, 2500, 5000mg/kg was 0.1±0.5, 0.5±0.5 and 0.4±0.5 respectively. Therefore, the test substance treatment did not cause any change in the frequency of micronucleated polychromatic erythrocytes when compared with the solvent control. Consequently, the micronucleus assay results confirmed that test substance at doses up to 5000 mg/kg did not exhibit any genotoxicity in mouse bone marrow.
Based on the data summarized, dimethyl sulfone, methanesulfonylmethane (67-71-0) did not induce gene mutation .Hence it is not likely to be mutagenic in vitro.
Justification for classification or non-classification
Thus based on the above annotation and CLP criteria the test chemical dimethyl sulfone, methanesulfonylmethane (67-71-0)did not induce gene mutation .Hence it is not likely to be mutagenic in vitro.
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