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EC number: 440-550-2 | CAS number: 244021-01-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed according to OECD-Guideline under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Details on test material:
- Identity: TKA 40270 (CGPS 345)
Batch no.: Lot 02243FC7, Op. 1/07
Purity: Confidential information (see Analytical Certificate No.38291-1)
Expiration date: 01-Oct-2012
Solubility in water: <0.21 mg/L
Aggregate state / physical form at room temperature: Liquid
Color: Colorless to yellow
Storage conditions: Storage at under 18 °C may lead to partial solidification. To reliquify, heat contents to 25-35 °C. Protect from light. (At RCC: At room temperature at about 20 °C, away
from direct sunlight.)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For measurement of the actual concentrations of the test item, duplicate samples were taken from
the test media of all test concentrations at the start of the test (without algae) and at the end of the
test (containing algae). At the same sampling times, duplicate samples were also taken from the
control. For the 72-hour stability samples, the contents of the test flasks of each treatment were
combined.
In addition, a vessel containing the undiluted filtrate was incubated without algae under test
conditions.
The samples were analyzed immediately after sampling. In order to stabilize the samples, 1 mL
acetonitrile per mL sample was added.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Due to the low water solubility of the test item, a dispersion with the loading rate of 100 mg/L was prepared by dispersing 150.5 mg of the test item
in 1500 mL of test water using intense stirring. No auxiliary solvent or emulsifier was used. The dispersion was stirred by a magnetic stirrer at room temperature in the dark over 96 hours to dissolve a maximum amount of the test item in the dispersion.
The stirring period of 96 hours was selected according to the results of a pre-test (non-GLP) which showed that the concentration of dissolved test
item was increasing over the stirring period of 96 hours.
After the 96-hour stirring period, the dispersion of the test item was filtered through a membrane filter (Schleicher & Schuell, Type NC45, pore size 0.45 µm). The undiluted filtrate was used as the highest concentrated test medium and as stock solution for the preparation of the test media of lower test concentrations. For the preparation of the test media of the lower test concentrations, the filtrate was diluted with test water. The test media were prepared just before the start of the test (= addition of algae).
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test organism used for the study was Scenedesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany). The algae were cultivated in RCCs laboratories under standardized conditions according to the test guidelines.
Due to the division of the genus Scenedesmus into the genera Scenedesmus and Desmodesmus, Scenedesmus subspicatus was transferred to the new genus Desmodesmus [2, 3]. Thus, Scenedesmus subspicatus was renamed as Desmodesmus subspicatus. However, the former name Scenedesmus subspicatus as specified in the guidelines is used in this report.
An inoculum culture was set up four days before the start of the test. The algae were cultivated under the test conditions. The inoculum culture was diluted threefold one day before the start of the test to ensure that the algae were in the exponential growth phase when used to inoculate the test solutions.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Exponentially growing cultures of this algal species were exposed to the test item over a period of 72 hours and
the inhibition of algal growth in relation to control cultures were assessed over several
generations.
Test conditions
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 22-23°C
- pH:
- The pH values was between 8.2 and 8.6 at the end of the test.
- Nominal and measured concentrations:
- Nomerial Concentration: 100 mg/L (loading rate) and dilutions of the filtrate 1:2.2, 1:4.6, 1:10, 1:22
Measured concentration: Undiluted filtrate: 0.35 mg/L; Dilution 1:2.2: 0.15 mg/L ; Dilution 1:4.6:- Details on test conditions:
- The test flasks were incubated in a temperature-controlled water bath at a temperature of 22-23 °C and illuminated by fluorescent tubes (Philips TLD 36W/840), installed above the test flasks. The mean measured light intensity at the level of the test solutions was approximately 5000 Lux (range:
4500 to 5400 Lux, measured at nine places in the experimental area).- Reference substance (positive control):
- yes
- Remarks:
- For evaluation of the algal quality and the experimental conditions, potassium dichromate is tested as a positive control twice a year to demonstrate satisfactory conditions of the test.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Details on results:
- The test item had no significant inhibitory effect on the growth of the algae (growth rate and yield) during the test period of 72 hours up to and
including the highest test concentration of 100 mg/L (loading rate ) corresponding to a measured concentrtion of the test item in the filtrate of 0.35 mg/L (results of Dunnetts tests, one-sided, a = 0.05).
The test concentration of 100 mg/L (loading rate) corresponding to a measured concentration of the test item in the fil.trate of 0.35 mg/L, thereforewas determined to be the 72-hour NOEC. The 72-hour LOEC was higher than 100 mg/l (loading rate). o.35 mg/L was representing the maximum
concentration of the test item, which could be dissolved in the test water. - Results with reference substance (positive control):
- The latest result of the positive control test in 2007 (72-h ECSO for the growth rate: 0.64 mg/L, RCC
Study No. B673S3) showed that the toxic performance was valid and within the historical range
ofthe RCC laboratory (from 1996 to 2007: 72-h ECSO: 0.44-1.16 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item TKA 40270 (CGPS 345) had no toxic effects on the algae up to its water solubility limit in test water under the present conditions of the test (corresponding to a loading rate of 100 mg/L).
- Executive summary:
The test item had no significant inhibitory effect on the growth of the algae (growth rate and yield) during the test period of 72 hours up to and including the highest test concentration of 100 mg/L (loading rate ) corresponding to a measured concentrtion of the test item in the filtrate of 0.35 mg/L (results of Dunnetts tests, one-sided, a = 0.05).
The test concentration of 100 mg/L (loading rate) corresponding to a measured concentration of the test item in the fil.trate of 0.35 mg/L, thereforewas determined to be the 72-hour NOEC. The 72-hour LOEC was higher than 100 mg/l (loading rate). o.35 mg/L was representing the maximum concentration of the test item, which could be dissolved in the test water.
The test item were analytically determined concentrations of the test item in the test media (dilution 1:2.2 and the undiluted filtrate) were 0.15 and 0.35 mg/L, respectively. During the test period of 72 hours, a decrease of test item concentration was determined in the presence of algae. At the end of the test, a concentration of 0.19 mg/L was determined in the undiluted filtrate with the loading rate of 100 mg/L. However, in samples from the undiluted filtrate incubated under the test conditions for 72 hours without algae, the test item was shown to be stable (measured concentration of 0.31 mg/L). Thus, the reason for the decrease of the test item in the samples containing algae was adsorption to algal cells. Consequently, the test item was not lost from the test system and the biological results are related to the initially measured test concentrations, as well as to the loading rate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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