main component 1 (isomer 1): 2-{6-fluoro-4-[3-(2,5-disulfo-phenylazo)-4-hydroxy-2-sulfonapht-7-ylamino]-1,3,5-triazin-2-ylamino}-3-{6-fluoro-4-[3-(1,5-disulfonaphth-2-ylazo)-4-hydroxy-2-sulfonaphth-7-ylamino]-1,3,5-triazin-2-ylamino}-propane sodium salt;main component 1 (isomer 2): 2-{6-fluoro-4-[3-(2,5-disulfo-phenylazo)-4-hydroxy-2-sulfonaphth-7-ylamino]-1,3,5-triazin-2-ylamino}-3-{6-fluoro-4-[3-(2,5-disulfo-phenylazo)-4-hydroxy-2-sulfonaphth-7-ylamino]-1,3,5-triazin-2-ylamino}-propane sodium salt;main component 2: 2,3-bis-{6-fluoro-4-[3-(2,5-disulfo-phenylazo)-4-hydroxy-2-sulfonaphth-7-ylamino]-1,3,5-triazin-2-ylamino}-propane sodium salt;main component 3: 2,3-bis-{6-fluoro-4-[3-(1,5-disulfonaphth-2-ylazo)-4-hydroxy-2-sulfonaphth-7-ylamino]-1,3,5-triazin-2-ylamino}-propane sodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 October 1996 to 28 November 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identity: FAT 40557/A
Batch-no.: PV1
Purity: 65 %
Appearance: Solid powder, red-brown
Solubility (g/l): >100
Expiration date: September, 2002
Storage conditions: At room temperature

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the fish bath at the beginning, after 48 hours and at the end of the experiment. The samples were taken out of the test solutions from the approximate center of the test vessels after mixing the medium. The samples were kept at -18 °C to -25 °C until analysis.

Vehicle:

yes

Details on test solutions:

0.5 g test substance was dissolved in 200 mL dechlorinated tap water, was homogenized for 5 minutes by ultrasonic treatment and then added up to 0.5 litre.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- The test organism was the species Zebra fish (Brachydanio rerio) supplied by Charles River Aquatics: Someren, Netherlands. The quality of the Zebra fish was checked with an acclimatization time of 12 days in dechlorinated tap water. For each concentration, 7 fish were used.
- Fish weight: 0.09 g (mean)
- Fish length: 23 mm (mean)
- Fish age: 109 days
- Treatment: none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

197 mg/L CaCO3

Test temperature:

20.6-21.4 °C

pH:

8.1 - 8.6

Dissolved oxygen:

98-99%

Nominal and measured concentrations:

Nominal: 100 mg/L. During the test the test article concentrations in the analysed test media were in the range of 97- 104 % of the nominal values.

Details on test conditions:

The fish were exposed in dechlorinated tab water (carbon filter) in 5 litre glass aquaria with a test volume of 2.88-2.96 litre (3.0 litre minus 6x 20 mL for analytical samples). During exposure the fish were kept at actually 20.5-21.4 °C, slowly aerated. Fish were not fed during the test. Lighting: yellow light 12 hours daily.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

In the blank control and at a test concentration of 100 mg/L no deaths occurred and no abnormal behaviour was noted.

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 value of FAT 40557/A to zebrafish is > 100 mg/L and is considered not toxic to fish.

Executive summary:

The acute toxicity of the test substance to zebrafish was determined in a GLP-compliant 96 hours static test according to the OECD Guideline 203. A range finding test was carried out at concentrations from 0.3 to 300 mg/L to determine the dose range for the main-test. The nominal concentration for the main-test was 100 mg/L (limit test). Analysis of the test media showed that concentrations maintained within 97- 104 % of the nominal value. In the control and the test concentration no mortalities or abnormal behaviour of the test fish was observed throughout the test. The LC50 value could not be determined, but was higher than 100 mg/L.

Description of key information

The 96-h LC50 value of test substance is >100 mg/L in zebrafish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

The acute toxicity of the test substance to zebrafish was determined in a GLP-compliant 96 hours static test according to the OECD Guideline 203. A range finding test was carried out at concentrations from 0.3 to 300 mg/L to determine the dose range for the main-test. The nominal concentration for the main-test was 100 mg/L (limit test). Analysis of the test media showed that concentrations maintained within 97- 104 % of the nominal value. In the control and the test concentration no mortalities or abnormal behaviour of the test fish was observed throughout the test. The LC50 value could not be determined, but was higher than 100 mg/L.