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EC number: 935-721-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012, Sept 10 - 2013, March 24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (1-phenylethyl)-phenyloxymethyloxirane
- IUPAC Name:
- (1-phenylethyl)-phenyloxymethyloxirane
- Test material form:
- other: yellow liquid
- Details on test material:
- - Name of test material (as cited in study report): Novares LR 600, (1-phenylethyl)-phenyloxymethyloxirane
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: liquid
- Analytical purity:
- Impurities (identity and concentrations):
- Composition of test material, percentage of components: Mixture of epoxidised mono- and distyrenated phenols (ratio 2:1)
- Isomers composition:
- Stability under test conditions:
- Storage condition of test material: Room temperature (20 +/- 5°C); keep away from light and humidity
- Other:
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal concentration < 5 mg/L in aqueous media: 100 mL of the solution in medium was extracted with 2 mL iso-octane, and the upper phase
was measured via GC/FID. 50-fold enrichment was achieved.
- Nominal concentration ≥ 5 mg/L but < 80 mg/L in aqueous media: 20 mL of the solution in medium was extracted with 2 mL iso-octane and the
upper phase was measured via GC/FID. Tenfold enrichment was achieved.
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- As the solubility of the test item in medium is limited (≤10 mg/L), water-accommodated fractions were prepared for the test. This was done by
weighing of the nominal loads, adding the corresponding amount of dilution water and shaking vigorously for 24 hrs.
The resulting solutions were filtrated through 0.45 µm filters.
- During validation of th analytical method, stability in test medium eithout alga cells after 72 h had been determined as 78 % (open system) and 77 % (closed system)
- Loss of test item in the main study might be also caused by uptake of the test item by algal cells.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular freshwater green alga, Desmodesmus subspicatus
- Strain: CHODAT
- Source: Stock culture (origin: MBM Sciencebridge GmbH, University of Göttingen in Oct 2011)
- Age of inoculum (at test initiation):
- Method of cultivation: Subcultivation from permanent culture on solid agar at 8 °C
ACCLIMATION
- Four days (96 h) before start of the test, pre-culture medium was inoculated with an aliquot of the stock culture.
- The resulting pre-culture was growing exponentially.
- Before usage the pre-culture was checked on the absence of cell aggregates
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no data
Test conditions
- Hardness:
- no data
- Test temperature:
- 23 °C - 34 °C
- pH:
- - Start of study: 7.6 - 7.8 in treatments, 8.0 in control (without test item)
- End of study: 8.5 - 9.1 in treatments, 9.3 in control (without test item) - Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal: 0.46 / 1.00 / 2.20 / 4.60 / 10.0 mg/L (loadings)
Measured: 0.50 / 1.02 / 1.83 / 2.67 / 6.37 mg/L at beginning of exposure - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks (50 ml nominal volume), unsealed
- Type: open
- Fill volume: 45 ml +/- 5 ml
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate): no data
- Initial cells density: 2206 cells/ml in each treatment
- Control end cells density: 176848 cells/ml (standard deviation: 15150) at 72 h
- No. of vessels per concentration (replicates): 3
- No. of vessels per positive control (replicates): 6
- No. of vessels per medium control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Photoperiod: no data
- Light intensity and quality: 5800 Lux
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Spectrophotometer; measurement of absorbtion (optical density) at 440 nm every 24 h
- Cell density was calculated based on the correlation curve between absorbtion and microscopic counts.
- Chlorophyll measurement: no data
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 (for loadings) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC
- Remarks on result:
- other: AUC: Area Under Curve = integral of the biomass (according to EU Method C.3)
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC
- Remarks on result:
- other: AUC: Area Under Curve = integral of the biomass (according to EU Method C.3)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC
- Remarks on result:
- other: AUC: Area Under Curve = integral of the biomass (according to EU Method C.3)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC
- Remarks on result:
- other: AUC: Area Under Curve = integral of the biomass (according to EU Method C.3)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: Yield: Biomass at the end minus biomass at the start of exposure (according to OECD Guideline 201)
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: Yield: Biomass at the end minus biomass at the start of exposure (according to OECD Guideline 201)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: Yield: Biomass at the end minus biomass at the start of exposure (according to OECD Guideline 201)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: Yield: Biomass at the end minus biomass at the start of exposure (according to OECD Guideline 201)
- Details on results:
- - Exponential growth in the control (for algal test): yes
- No inhibition was observed up to 2.2 mg test item per L (nominal; 1.3 mg/L measured) - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- The EC50 values of potassium dichromate (positive control) tested in a seperate reference test (LAUS study no. 2012 06R301) lay within the
normal range of the laboratory.
- The data showed a statistical sufficient dose-response- relation - Reported statistics and error estimates:
- The measured concentrations at the start of the test tended to decrease in a dose related manner (range between 58 % and 109 %).
Therefore, the determination of biological results was based on the geometric mean of the measured concentrations.
For the estimation of the EC50 a sufficient statistical dose-response-relation was found.
Any other information on results incl. tables
Due to the limited solubility of the test item the correlation between nominal and measured concentrations - especially at the higher concentrations - was weak (see table below). This was observed in both analysed peaks.
Following tables are excerpts from the original report:
Table 10.1-d: Measured concentrations (in mg/L) and corresponding recovery rates (in %)
Nominal conc. |
Measured conc. 0 h |
% Nominal conc. |
Measured conc. 72 h |
% Nominal conc. |
% Recovery |
Geometric Mean |
mg/l |
mg/L |
% |
mg/ml |
% |
% |
mg/ml |
Control |
< LOQ |
- |
< LOQ |
- |
- |
- |
0.46 |
0.50 |
108,70 |
0.32 |
69.57 |
64.00 |
0.40 |
1 |
2.02 |
101.5 |
0.59 |
58.50 |
57.64 |
0.77 |
2.2 |
1.83 |
82.95 |
0.97 |
43.86 |
52.88 |
1.33 |
4.6 |
2.67 |
58.04 |
2.26 |
49.13 |
84.64 |
2.46 |
10 |
6.37 |
63.65 |
5.18 |
51.80 |
81.38 |
5.74 |
Table 10.1-a: Cell Number/mL (Main Study)
Nominal Concentration in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
0 |
Mean |
2206 |
7905 |
26104 |
176848 |
0 |
SD |
0 |
450 |
4662 |
15150 |
0.46 |
Mean |
2206 |
6618 |
26840 |
169127 |
0.46 |
SD |
0 |
1103 |
3874 |
36086 |
1 |
Mean |
2206 |
6986 |
27575 |
161773 |
1 |
SD |
0 |
637 |
2206 |
10008 |
2.2 |
Mean |
2206 |
8456 |
31987 |
165818 |
2.2 |
SD |
0 |
637 |
1103 |
13343 |
4.6 |
Mean |
2206 |
7721 |
23531 |
75739 |
4.6 |
SD |
0 |
1103 |
4592 |
5213 |
10 |
Mean |
2206 |
5883 |
7353 |
8089 |
10 |
SD |
0 |
637 |
3184 |
2776 |
SD = Standard Deviation
Table 10.1-e: Growth Rate µ, Area under the Curve AUC, Yield Main Study (0 – 72 h)
Nom. Conc. in mg/L |
Meas. Conc. in mg/L (geom. mean) |
Growth Rate [day-1] |
AUC |
Yield [Cell Conc./mL] |
|
0 |
Mean |
1.46 |
116918 |
174642 |
|
SD |
0.03 |
11982 |
15150 |
||
0.46 |
0.40 |
Mean |
1.44 |
112506 |
166921 |
SD |
0.07 |
15898 |
36086 |
||
1 |
0.77 |
Mean |
1.43 |
109932 |
159567 |
SD |
0.02 |
6851 |
10008 |
||
2.2 |
1.33 |
Mean |
1.44 |
117837 |
163612 |
SD |
0.03 |
6392 |
13343 |
||
4.6 |
2.46 |
Mean |
1.18 |
63606 |
73533 |
SD |
0.02 |
3324 |
5213 |
||
10 |
5.74 |
Mean |
0.42 |
11765 |
5883 |
SD |
0.12 |
4284 |
2776 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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