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EC number: 629-620-5 | CAS number: 13534-99-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 2016 - Feb 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-bromopyridin-2-amine
- EC Number:
- 629-620-5
- Cas Number:
- 13534-99-1
- Molecular formula:
- C5 H5 Br N2
- IUPAC Name:
- 3-bromopyridin-2-amine
- Test material form:
- solid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly industrial, non-adapted
- Details on inoculum:
- The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant.
The freshly obtained sludge was preconditioned to experimental conditions by continuous aeration until further treatment. The concentration of suspended solids was determined to be 3.7 g/L in the concentrated. Before use, the sludge was allowed to settle (49 minutes) and the
supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium. - Duration of test (contact time):
- >= 0 - <= 28 d
Initial test substance concentration
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Pre-incubation medium The day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and
inoculum (1% of final volume) were added to each bottle.
This mixture was aerated with synthetic air overnight to purge the system of CO2.
Type and number of bottles Test suspension: containing test item and inoculum (2 bottles).
Inoculum blank: containing only inoculum (4 bottles total;
2 bottles as blanks for the positive control aeration line and
2 bottles as blanks for the test item aeration line)
Positive control: containing reference item and inoculum (1 bottle).
Toxicity control: containing test item, reference item and inoculum (1 bottle).
Preparation At the start of the test (day 0), test and reference item were added to the bottles containing the microbial organisms and mineral components.
The volumes of suspensions were made up to 2 litres with Milli-RO water, resulting in the mineral medium described before.
Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle.
Results and discussion
- Test performance:
- Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test suspension.
Titrations for the positive and toxicity control were made over a period of 14 days.
Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers was moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series.
Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
On day 28, the pH of the test suspensions associated with the test item and toxicity control was measured. Thereafter, 1 mL of concentrated HCl (37%, Merck) was added to the bottles of the inoculum blank and test suspension. The bottles were aerated overnight to drive off CO2 present in the test suspension. The final titration was made on day 29.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 29 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9
- Sampling time:
- 29 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of QAW039-C1 (2% and 9%, based on ThCO2).
In the toxicity control, more than 25% biodegradation occurred within 14 days (30%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.
Functioning of the test system was checked by testing the reference item sodium acetate, which showed a normal biodegradation curve.
Any other information on results incl. tables
The temperature recorded in a vessel with water in the same room varied between 21.7 and
22.4°C.
The pH values of the different test media are 7.5 - 7.9
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- QAW039-C1 was not readily biodegradable under the conditions of the modified Sturm test presently performed.
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