2,2-dimethylpropan-1-ol, tribromo derivative; 3-bromo-2,2-bis(bromomethyl)propan-1-ol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2003-2004

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP study conducted in an ISO 9001 lab using appropriate guidelines

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Principles of method if other than guideline:

Pre exposure test based on OECD guideline 302D was introduced where the inoculum was incubated during a period of few weeks with incremental additions of FR-513. thereafter the pre-exposed inoculum was used for the biodegradability test.

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not relevant

Oxygen conditions:

aerobic

Inoculum or test system:

other: 14:1 activated sludge/industrial sludge

Details on inoculum:

The inoculum used was a composite inoculum consisting of a mixture of activated sludge from Haifa municipal WWTP and sludge from an industrial WWTP (Broomchemie). The inoculum was prepared by mixing the sludge at a ratio of 14:1 activated sludge/industrial sludge.

Duration of test (contact time):

36 d

Initial conc.:

200 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

see attached file

Reference substance:

benzoic acid, sodium salt

Preliminary study:

not conducted

Test performance:

see results below

Parameter:

% degradation (DOC removal)

Value:

77

Sampling time:

36 d

Details on results:

The results from the study indicate that FR-5 13 is inherently biodegradable. Under the test conditions ultimate biodegradation of FR-5 13 at an extent of 77% was attained during a period of 36 days. The deviations between the replicates were less than 20% and therefore the results are considered valid. The validity of the test was demonstrated by the results of the procedure control where 90% DOC removal was attained in the first week.

Results with reference substance:

During the first 10 days of incubation, a decrease of 90% in DOC concentration was observed in the procedure control. Therefore the test can be defined as valid (over 70% degradation in 14 days).

see attached document on tables

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The results indicate that FR-513 can be defined as inherently biodegradable. An average decrease of 77% in DOC concentrations was observed in the test reactions in a period of 36 days, following a lag period of approx. a week.

Executive summary:

The present study was designed to assess the potential for inherent biodegradability of Tribromoneopentyl alcohol (FR-513) under aerobic conditions. The test was conducted according to OECD guidelines 302B. Prior to the biodegradability test, a pre-exposure stage based on OECD guidelines 302D was introduced in order to enrich the inoculum for bacterial populations capable of using FR-513. The concentration of FR-513 in the test was 200 mg/L (35 mg/L dissolved organic carbon DOC). Four controls were tested in parallel: a blank control without FR-513, a procedure control were the degradation of the reference substance sodium benzoate was analyzed, an inhibition control where the effect of FR-513 on sodium benzoate degradation was evaluated and abiotic poisned control where mercuric chloride was amended. The biodegradation process was followed by periodical analyses of DOC concentrations.

The results indicate that FR-513 is inherently biodegradable. Under the test conditions ultimate biodegradation of FR-513 at an extent of 77% was attained during a period of 36 days. The deviatonsbetween the replicates were less than 20% and therefore the results were considered valid. The validity of the test was demonstrated by the results of the procedure control where 90% DOC removal was attained in the first week.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and appropriate guidelines

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3120 (Sealed Vessel Carbon Dioxide Production Test)

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not relevant

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

The inoculum used for the test consisted of activated sludge from Haifa municipal wastewater treatment plant (WWTP). The sludge was collected three days prior to the initiation of the experiment and aerated during three days to reduce carbonaceous background. The day before the experiment was started, the sludge was diluted in the mineral medium to yield a final mixed liquor suspendedsolids (MLSS) concentration of 100 mg/L, and then continued to be aerated during the night with CO2 free air.

Duration of test (contact time):

28 d

Initial conc.:

110 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

other: inorganic carbon evolution

Details on study design:

see attached file on study design

Reference substance:

benzoic acid, sodium salt

Preliminary study:

not relevant

Test performance:

not relevant

Parameter:

% degradation (CO2 evolution)

Value:

2.5

Sampling time:

28 d

Details on results:

see attached document on results

Results with reference substance:

sodium benzoate degradation extent was 84% during the first 15 days of incubation , exceeding the minimal 60% required for the test to be valid, and was completly mineralized by the end of test.

not available

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The results from biodegradability test indicate that FR-513 is not readily biodegradable. The test was proven valid since sodium benzoate was mineralized by an average 84% during the first 14 days of the tst and the mean TIC present in the blank control at the end of the test was less than 15% of the organic carbon added initially as the test substance. In addition, FR-513 was not inhibitory to the biomass at the test conditions.

Executive summary:

A ready biodegradibility test was performed for tribromoneopentyl alcohol (FR-513). Study was conducted usin OECD guideline 310 and OPPTS guideline 835.3120. The biodegradation extent was determined by measuring the CO2 evolution resulting from the ultimate biodegradation (mineralization) of the test compound. The results from biodegradability test indicate that FR-513 is not readily biodegradable, since in the samples containg FR-513 the CO2 produced during 28 days was equivalent to an extent of 2.5% ultimate biodegradation. The test was proven valid since sodium benzoate was mineralized by an average 84% during the first 14 days of the tst and the mean TIC present in the blank control at the end of the test was less than 15% of the organic carbon added initially as the test substance. In addition, FR-513 was not inhibitory to the biomass at the test conditions.

Description of key information

Inherently biodegradable
Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater

Additional information

Inherent biodegradability test was conducted under aerobic conditions according to OECD guidelines 302B/302D.

The results indicate that FR-513 is inherently biodegradable. Under the test conditions ultimate biodegradation of FR-513 at an extent of 77% was attained during a period of 36 days. The deviatons between the replicates were less than 20% and therefore the results were considered valid. The validity of the test was demonstrated by the results of the procedure control where 90% DOC removal was attained in the first week.

 

 

Ready biodegradibility test was conducted using OECD guideline 310 and OPPTS guideline 835.3120. The results from biodegradability test indicate that FR-513 is not readily biodegradable, since in the samples containg FR-513 the CO2 produced during 28 days was equivalent to an extent of 2.5% ultimate biodegradation. The test was proven valid since sodium benzoate was mineralized by an average 84% during the first 14 days of the tst and the mean TIC present in the blank control at the end of the test was less than 15% of the organic carbon added initially as the test substance. In addition, FR-513 was not inhibitory to the biomass at the test conditions.