N-(2-nitrophenyl)phosphoric triamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-01-17 - 2005-07-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

Preparation of the test solution
The test solution was prepared in the following way:
- Weighing of 1000 mg test item
- Adding of dilution water up to a final volume of 1000 mL (stock solution)
- Filling with dilution water up to a final volume of 10 L
At the start of the test, the test item pool contained 10 L test solution with the test item at a nominal concentration of 100 mg/L dilution water. The control pool contained 10 L dilution water only.
The dilution water was prepared some days before the start of the test and stored without further aeration before use in the same cabinet and at the same temperatures as the fish test was performed. The pools were permanently aerated by an aeration pump.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Origin of the test organism:purchased from ZOO KNAPPIG, Sachsenpark, An der Passage 1, D-04356 Leipzig
Origin of the fish used in the test: holding and breeding in the testing facility under standardised laboratory conditions
Total length of the test fish: 2.4-3.0 cm

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

2 mmol/L CaCO3, which corresponds to a hardness of 200 mg/l CaC03

Test temperature:

21.7- 22.5 °C

pH:

7.39 - 7.66

Dissolved oxygen:

8.66 - 8.95 mg/l

Nominal and measured concentrations:

see below

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Cumulative mortality after 3, 6, 24, 48, 72 and 96 hours
Number of dead fish
Treatment group 3 h 6 h 24 h 48 h 72 h 96 h
Control (dilution water) 0 0 0 0 0 0
100 mgP 101/04/L 0 0 0 0 0 0

Sublethal observations / clinical signs:

Treatment group	Analysed content of P 101/04 (mgP 101/04/L)
	Oh	96 h
Control (dilution water)	0	0
100 mgP 101/04/L	100.9	100.2

Treatment group	Dissolved oxygen (mg/L)
	Oh	24 h	48 h	72 h	96 h
Control (dilution water)	9.10	8.24	9.04	8.53	8.97
100 mg P 101/04/L	8.93	8.72	8.66	8.95	8.83

Treatment group	pH value
	Oh	24 h	48 h	72 h	96 h
Control (dilution water)	7.44	7.32	7.59	7.24	7.06
lOOmgP 101/04/L	7.61	7.66	7.61	7.59	7.39

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the described study no effects of P 101/04 on the mortality of fish were determined. The NOEC (no observed effect concentration) after 96 hours was 100 mg P 101/04 /L.

Executive summary:

In a 96-h acute toxicity study, zebra fish (Danio rerio) were exposed to N- (2 -nitrophenyl) -phosphoric triamide at a nominal concentration of 100 mg/l under static conditions.

No effects of P101/04 on the mortality of fish were determined. The NOEC (no observed effect concentration) after 96 hours was 100 mg P 101/04 /L.

The validity criteria were accomplished:

Mortality at the end of the test:   < 10 % in the control (observed: 0 %)

Test conditions:                              constant (observed: constant)

Oxygen concentration:                  > 60 % of the air saturation value throughout the test (observed: > 91 %)

Description of key information

OECD 203 guideline study, GLP: NOEC: 100 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

100 mg/L

Additional information

In a 96-h acute toxicity study according to OECD guideline 203, zebra fish (Danio rerio) were exposed to N- (2 -nitrophenyl) -phosphoric triamide at a nominal concentration of 100 mg/l under static conditions.

No effects of N-(2-Nitrophenyl)phosphoric triamide on the mortality of fish were determined. The NOEC (no observed effect concentration) after 96 hours was 100 mg P N-(2-Nitrophenyl)phosphoric triamid/L.