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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The data was evaluated by experts in a report of NATIONAL INDUSTRIAL CHEMICALS NOTIFICATION AND ASSESSMENT SCHEME(NICNAS), which is administered by the National Occupational Health and Safety Commission (NOHSC) of Australian Commission. Also the Data were determined by the OECD guideline.

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
Phosphoric acid, (1-methylethylidene) di-4,1-phenylene tetraphenyl ester (Fyrolflex BDP)
Author:
National Occupational Health and Safety Commission (Australia)
Year:
2000
Bibliographic source:
In:NATIONAL INDUSTRIAL CHEMICALS NOTIFICATION AND ASSESSMENT SCHEME
Reference Type:
other company data
Title:
Unnamed
Year:
1997
Reference Type:
other: monograph provided by ECHA
Title:
Endpoint record:Short term toxicity to aquatic algae and cyanobacteria
Author:
ECHA
Year:
2010
Bibliographic source:
Document from ECHA

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Six replicate tests were conducted in 250 mL Erlenmeyer flasks, together with three control flasks containing no chemical. Three solvent controls (ie water and the appropriate amount of dimethylformamide) were also run. Each flask contained 100 mL of the test medium, and the flasks were continuously agitated to maintain the algal cells in suspension.

Test solutions

Vehicle:
yes
Details on test solutions:
The test substance was firstly dissolved in dimethylformamide to produce a 10 g/L solution. An aliquot of this was added to the test vessels in order to produce the indicated nominal concentrations.

Test organisms

Test organisms (species):
Selenastrum sp.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Hardness:
around 100 mg/L as CaCO3
Test temperature:
21 ± 1°C
pH:
The pH of the test solutions increased from around 8.0 at the start of the period, to around 10 after 72 hours.
Dissolved oxygen:
between 9.4 and 10.1 mg/L
Nominal and measured concentrations:
Analysis of the solutions for the test compound after 72 hours indicated approximately 74% recovery (compared with a nominal initial concentration of 1 mg/L).

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 1 mg/L
Nominal / measured:
nominal
Details on results:
The growth of algal biomass was determined over the test period by measurement of cell density using absorbance at 665 nm. At 0 hours the mean cell density in the control was 18,500 cell/mL, increasing to 2,170,000 cells/mL after 72 hours. The corresponding values for the solvent control were 21,700 and 1,300,000 cell/mL, respectively. While the report did not detail the actual cell densities for the test media, the absorbance closely paralleled that of the controls (and solvent control). While the report did not detail the actual cell densities for the test media, the absorbance closely paralleled that of the controls (and solvent control), and consequently it was concluded that the BDP is not inhibitory to growth of algae up
to the limits of its water solubility.

Any other information on results incl. tables

It was concluded that the BDP is not inhibitory to growth of algae up to the limits of its water solubility.

Analysis of the solutions for the test compound after 72 hours indicated approximately 74% recovery (compared with a nominal initial concentration of 1 mg/L). This suggests that some of the test material had adsorbed to the surface of the algal cells.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
An OECD guideline test by peer reviewed
Conclusions:
It was concluded that BDP is not inhibitory to growth of algae up to the limits of its water solubility.