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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: This study was conducted according to the methods of Annex V, Part B.1. (acute toxicity (oral), to Directive 67/548/EEC of the Council of the European Communities of June 27, 1967 (Official Journal of the European Communities L196/1 of August 16, 1967)
Principles of method if other than guideline:
This study was conducted according to the methods of Annex V, Part B.1. (acute toxicity (oral), to Directive 67/548/EEC of the Council of the European Communities of June 27, 1967 (Official Journal of the European Communities L196/1 of August 16, 1967) in the current version as amended for the seventeenth time by Directive 92/69/EEC of the Commission of the European Communities of July 31, 1992 (Official Journal of the European Communities L 383 A of December 29, 1992).
GLP compliance:
yes
Test type:
other: Acute oral toxicity
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Tris(2-chlorisopropyl)phosphat CAS-No. 13674-84-5
designation: tris(2-chloropropyl)phosphate
manufacturer: Bayer AG
Batch: 07026
CAS no: 13674-84-5
appeartance: colourleaa liquid
storage: room temperature
expiry date: 07 Feb 1998

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: corn oil
Doses:
200, 500 mg/kg (both sexes) 2000mg/kg females only
No. of animals per sex per dose:
5/sex/dose
only female rats received 2000 mg/kg bw.
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
632 mg/kg bw
Sex:
male
Dose descriptor:
LD50
Effect level:
> 500 - < 2 000 mg/kg bw
Mortality:
All animals in the female group 2000 mg/kg bw died after 3-6 hours.
Clinical signs:
In female rats after 2000 mg/kg bw following signs of intoxication were observed: Apathy, palmospasm, blood-crusted snout and lateral position.
A single dose of 200 and 500 mg/kg bw was tolerated by male and female rats without any clinical signs.
Body weight:
Growth of male and female rats was not affected.
Gross pathology:
At necropsy mottled reddened lungs were noticed in the animals which died during the observation period.
None of the animals sacrified at the end of the 14-day observation period showed any noticeable gross pathological findings.

Any other information on results incl. tables

RS-Freetext:
LD50 > 500 mg/kg (males); LD50 = 632 mg/kg (females)

RS-Freetext:
mortality: 0/5 at 200 and 500 mg/kg (both sexes), 5/5 at
2000 mg/kg (females); clinical signs observed only at 2000
mg/kg: apathy, palmospasm, blood-crusted snout and lateral
position; necropsy findings only at 2000 mg/kg: reddened
lungs

Applicant's summary and conclusion

Executive summary:

Acute toxicological investigations in male and female Wistar rats were conducted after single oral administration of TCPP.

The LD50 was calculated as approximate 632 mg/kg body weight for female rats and was greater than 500 mg/kg and less than 2000 mg/kg bw for the male rats.