Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 451-160-7
CAS number: 17913-76-7
The study was conducted according to a method that was designed to
assess the potential mutagenicity of the test material on the thymidine
kinase, TK +/-, locus of the L5178Y mouse lymphoma cell line, >>The
method used meets the requirements of the OECD (476) and the Method B17
of Commission Regulation (EC) No. 440/2008 of 30 May 2008.
Two independent experiments wre performed. In Experiment 1, L5178Y TK
+/- 3.7.2c mouse lymphoma cells (heterozyous at the thymidine kinase
locus) were treated with the test material at up to eight dose levels,
in duplicate, together with vehicle (solvent) and positive controls
using 4 -hour exposure groups both in the asence and presence of
metabolic activation (2% S9). In experiment 2, the cells were treated
with the test material at up to eight dose levels using a 4 -hour
exposure group in the absence of metabolic activation.
The dose range of test material was selected following the results of a
preliminary toxicity test and for the first experiment was 4.38 to 140
µg/ml in the absence of metabolic activation, and 17.5 to 280 µg/ml in
the presence of metabolic activation. For the second experiment the dose
range was 4.38 to 140 µg/ml in the absence of metabolic activation, and
17.5 to 210 µg/ml in the presence of metabolic activation.
THe maximum dose level used in the mutagenicity test was limited by test
material-induced toxicity. Precipitate of the test material was not
observed at any of the dose levels in the mutagenicity test. The vehicle
(solvent) controls had acceptable mutant frequency values that were
within the normal range for the L5178Y cel line at the TK +/- locus. The
positive control materials induced marked increases in the mutant
frequency indicating the satisfactory perfomance of the test and of the
activity of the metabolising system.
The test material did not induce any toxicologically significant
dose-related increases in the mutant frequency at any dose level, either
with or without metabolic activation, in either the first or the second
The test material was considered to be non-mutagenic to L5178Y cells
under the conditions of the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Esta web utiliza cookies para mejorar su experiencia de navegación en nuestros sitios web.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again