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EC number: 480-060-6 | CAS number: 24065-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Okt - 8 Nov 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV/VIS
- Details on sampling:
- - Sampling method: Aliquots of the samples from the biological test were directly analyzed by HPLC and UV/VIS-detection (range of the injection volume: 1-100 µL, depending on the expected concentration).
- Sample storage conditions before analysis: Routinely, the samples are analyzed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the test concentration, 105.2 mg of the test substance were added to 1 L of dilution water, treated for 1 hour in an ultrasonic bath and then stirred for 24 h on a magnetic stirrer.
- Controls: 1 control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: parthenogenetic females
- Age at study initiation: 0-24 h
- Stage and instar at study initiation: Neonates
- Method of breeding: well-established test culture
- Source: Bundesgesundheitsamt Berlin
- Feeding during test: no
ACCLIMATION
- Acclimation period: The culture water (so called 'M4 medium') is partly renewed once a week.
- Type and amount of food: The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS
- The neonates are separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 15.7°dH (=280.25 mg/L CaCO3)
- Test temperature:
- 20.4 to 20.5 °C
- pH:
- 7.9 to 8.3
- Dissolved oxygen:
- 8.6 mg/L
- Nominal and measured concentrations:
- 100 mg/L (nominal concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- Volume of solution: 20 mL of test medium
- Aeration: no
- No. of organisms per vessel: 10 neonates
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (M4-medium)
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
- Light intensity: < 1000 lux
EFFECT PARAMETERS MEASURED
- Immobilization rates are recorded at 24-hour intervals - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Calculated on the basis of EC0 ≥ 100 mg/L (nominal concentration).
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Immobilisation of control: 0%
- Immobilisation with test substance: 0% - Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- EC50 (48 h) >100 mg/L
- Executive summary:
The study was performed to assess the acute toxicity of the test substance to Daphnia magna under static conditions. The study was conducted in accordance with 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I- The 24h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath and a magenetic stirrer. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: EC0>/=100 mg/L nominal concentration (48 h). Recovery rates correspond to 104.9% of nominal value at 0 hours and to 98.7% of nominal value at 48 hours. The EC50 was derived from the raw data since no effects were observed.
Reference
Chemical analysis of the test substance
Chemical analysis | 0 h | 48 h |
HPLC replicates [mg/L] | 105.918/ 103.977 | 98.648/ 98.823 |
HPLC mean values [mg/L] | 104.95 | 98.74 |
Chemical analysis of the control
Chemical analysis | 0 h | 48 h |
HPLC replicates [mg/L] | <0.064/ <0.064 | <0.064/ <0.064 |
HPLC mean values [mg/L] | <0.064 | <0.064 |
Validity criteria for the measurement of the daphnia toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | Immobilization of control: 0% | Yes |
The dissolved oxygen concentration at the end of the test should be >/= 3 mg/L in control and test vessels. | Dissolved oxygen concentration was 8.6 mg/L in control and test vessels. | Yes |
The concentration of the test item shall be maintained to within 80% of the initial concentration throughout the study. The maintenance of concentration is proved by analytical measurements | Recovery rates correspond to 104.9% of nominal value at 0 hours and to 98.7% of nominal value at 48 hours. | Yes |
A limit test may be performed at 100 mg/L of the test substance in order to demonstrate that the EC50 is greater than this concentration. The limit test should be performed using 20 daphnids, with the same number in the control. If the percentage of immobilisation exceeds 10% at the end of the test, a full study chould be conducted. | Within the limit test, 20 daphnids were used for the test concentration of 100 mg/L (2 replicates and 10 daphnia per test vessel) and 20 daphnids for the control (2 replicates and 10 daphnia per test vessel). No immobilization of daphnids was observed within the test. | Yes |
Description of key information
A study was performed to assess the acute toxicity of the test substance to Daphnia magna under static conditions. The study was conducted in accordance with 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I- The 24 h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath and a magenetic stirrer. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: EC0 ≥ 100 mg/L nominal concentration (48 h). Recovery rates correspond to 104.9% of nominal value at 0 hours and to 98.7% of nominal value at 48 hours. The EC50 was derived from the raw data since no effects were observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- Calculated on the basis of EC0 ≥ 100 mg/L (nominal concentration).
- Effect concentration:
- > 100 mg/L
Additional information
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