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EC number: 605-399-0
CAS number: 165252-70-0
Table 1: Deviations from the study plan
Section of study plan
Impact on the study
2.1 Test item
The date of the certificate of analysis changed from 05 September 2011 to 24 November 2010.
Mistake during study plan preparation.
2.2 Reference item
The stock solution of the reference item was not prepared with 0.5 g with a final volume of 500 mL but with 0.25 g filled up to a final volume of 250 mL.
Mistake during study plan preparation
None, since the concentration was the same
2.5 Microbial Inoculum
The sludge was not used on the same day as collec-tion but on the next day.
Mistake in study performance
None, since the guideline allows storage up to two days
The sludge was not centrifuged for 20 min at 4500 rpm but for 10 min at 3000 rpm.
Mistake in study performance.
None, since the sludge was centrifuged to produce a clear supernatant and pellet of sewage solids, therefore fulfilling guideline criteria as the guide-line does not stipulate a speed of centrifugation.
2.8 Test conditions
The temperature of the test decreased to 17.6 °C.
None, since the deviation did not have an adverse effect on the validity and outcome of the study. The guideline recommends a temperature range of 20 ± 2 °C. The lowest recorded temperature was 17.6°C. However, the test item showed no adverse effect and the EC50 value of the reference item was within the recommended range. Therefore, the temperature difference of 0.4°C is considered not to have had an adverse effect on the validity and the outcome of the study.
2.7.2 Procedure of the Test
The pH measured ranged from 7.92 – 8.22. This is above the recommended range of 7.5 ± 0.5.
None, since the deviation did not have an adverse effect on the validity and outcome of the study. The test item showed no adverse effect and the EC50 of the reference item was within the recommended range. Therefore, the pH difference of 0.2 is considered not to have had an adverse effect on the validity and the outcome of the study.
The test item dinotefuran technical had no adverse effects on the oxygen
uptake at the highest concentration tested (1000 mg/L). Hence, the EC50
of dinotefuran technical is > 1000 mg/L and the NOEC is 1000 mg/L.
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