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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22/11/1999 - 10/02/2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP, Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: granule

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At 0-hour and 48 hour

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Strain: Daphnia magna Straus
Age: 6-24 hours old
Breeding method: The Daphnids from the clone line were bred in the laboratories of RCC in reconstituted water under identical conditions as in the tests.
Kind of food: Not reported
Amount of food: Not reported
Feeding frequency: Not reported
Pretreatment: No
Feeding of animals during test: No

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
1.25 mmol/L (= 125 mg/L) as CaCO3
Test temperature:
20 – 21 ºC
pH:
7.7 – 7.8
Dissolved oxygen:
8.7 mg/L
Salinity:
not applicable
Nominal and measured concentrations:
See table below: Nominal and measured concentrations
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The analytically determined mean test item concentrations at the start and the end of the test were 97% of the nominal value. The test item MTI-446 was stable under the test conditions during the test period of 48 hours. Therefore all results are reported as nominal test concentrations. In the control (0 mg/L) and the test item concentration of 1000 mg/L, no immobilized or dead test organisms or other signs of intoxication were determined during the entire test period (48 hours).

The 48-hour NOEC and the 48-hour EC0 of dinotefuran to Daphnia magna were determined to be ≥ 1000 mg/L. The 48-hour EC50 and the 48-hour EC100 were >1000 mg/L.

No remarkable observations were made concerning the appearance of the test medium. It was a clear solution throughout the whole test duration. At the beginning and the end of the test period, the oxygen concentrations in the test medium and the control was 8.7 mg/L and the pH-values ranged from pH 7.7 - 7.8.
The EC50 and EC100 values (with 95% C.I.) could not be calculated due to insufficient immobility. The NOEC and LOEC were determined visually.

See Tables 1 and Table 2.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
The NOEC and EC0 were determined directly from the raw data.

Any other information on results incl. tables

Nominal and measured concentrations:

Nominal test

Concentration (mg/L)

Sample

(h)

Measured Concentration

(mg/L)

1000

0

0

965.8

974.8

48

48

964.7

967.8

Table 1:           Immobility of dinotefuran on Daphnia magna

Treatment

mg/L

(Nominal conc.)

Observation period

24 hours

48 hours

endpoint

% affected

endpoint

% affected

Dilution water Control

Immobile

0

Immobile

0

Solvent Control

N/A

N/A

N/A

N/A

Positive Control, if used

N/A

N/A

N/A

N/A

968.3 (1000)

Immobile

0

Immobile

0

 

Table 2:          Effect data of dinotefuran on Daphnia magna

Treatment

mg/L

(Nominal conc.)

Observation period

24 hours

48 hours

NOEC, mg/L

≥1000

≥1000

LOEC, mg/L

>1000

>1000

EC50(with 95% C.I.), mg/L

>1000

>1000

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 was > 1000 mg/L, which categorizes dinotefuran as nontoxic to the water flea (Daphnia magna) on an acute toxicity basis. The 48-hour NOEC and LOEC levels were ≥ 1000 and > 1000 mg/L, respectively.