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EC number: 251-311-5 | CAS number: 32961-44-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-06-23 - 2016-08-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- version dated 1992-07-17
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Mainzer Straße 80, D-65189 Wiesbaden
Test material
- Reference substance name:
- Isobutyl 4-chloro-3,5-diaminobenzoate
- EC Number:
- 251-311-5
- EC Name:
- Isobutyl 4-chloro-3,5-diaminobenzoate
- Cas Number:
- 32961-44-7
- Molecular formula:
- C11H15ClN2O2
- IUPAC Name:
- 2-methylpropyl 3,5-diamino-4-chlorobenzoate
- Test material form:
- solid
- Remarks:
- dark brown
- Details on test material:
- - Density: 1.106 g/cm³ (25°C)
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At ambient temperature, protected from light
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage plant at Taunusstein-Bleidenstadt
- Preparation of inoculum for exposure: It was washed twice with mineral nutrient solution of the CO2-Evolution-Test to eliminate organic components and carbonates from the sludge. After resolution with mineral nutrient medium the sludge was aerated by means of compressed humidified air for about four hours. Before use as inoculum for the CO2-Evolution-Test the sludge was homogenised in a "Waring Blender" at low speed for 2 minutes and then filtered through a cotton filter previously carefully rinsed with deionised water. The filtrate was used as inoculum (1% of the final volume of the test solution) on the same day of preparation. - Duration of test (contact time):
- 29 d
Initial test substance concentration
- Initial conc.:
- 19.26 mg/L
- Based on:
- TOC
- Remarks:
- According to the carbon content of the test item the amount of test item to be applied was calculated to give a final concentration of 10 to 20 mg TOC/L (Total Organic Carbon): 67.4 mg / 3.5 L / ca.10 mg TOC/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
For preparation of the test solutions the follo¬wing stock solutions of mineral nutrient salts were prepared:
8.50 g KH2PO4
21.75 g K2HPO4
33.40 g Na2HPO4 · 2 H2O
0.50 g NH4Cl
were diluted in 1 L Ultrapure Water [Seral, Purelab plus] (this solution may be stored for at least 3 month at +2 to +8 °C).
36.40 g CaCl2 · 2 H2O (corresp. 27.5 g CaCl2)
were diluted in 1 L Ultrapure Water [Seral, Purelab plus] (this solution may be stored for at least 3 month at +2 to +8 °C).
22.50 g MgSO4 · 7 H2O
were diluted in 1 L Ultrapure Water [Seral, Purelab plus] (this solution may be stored for at least 3 month at +2 to +8 °C).
0.25 g FeCl3 · 6 H2O
were diluted in 1 L Ultrapure Water [Seral, Purelab plus] (this solution was prepared freshly before use in the test series).
The test solutions contained in a total volume of 3500 mL: 35 mL of the inoculum, 3.5 mL of the ferrichloride solution, 3.5 mL of the magnesium sulphate solution, 3.5 mL of the calcium chloride solution, and 35 mL of the phosphate mixture solution. The test item was given directly into the test solutions. At time t0 67.4 mg of the test item were given into the first treatment and 67.7 mg of the test item was given into the second treatment at time t0. The final (calculated) concentration in the test solutions was ca 10 mg C/L.
- Test temperature: 20-21°C
- Aeration of dilution water: yes
TEST SYSTEM
- Culturing apparatus: 5-litre amber carboys served as test vessels. They were closed with stoppers with tubing for gas inlet and outlet (gas exit line). The gas outlet (exit air line) was connected to at least three CO2 absorber bottles filled with 100 mL 0.025 N Ba(OH)2 (connection in series), and bubbling of CO2 free compressed air through the solution was continued. Periodically (if necessary) the CO2 absorber nearest the test vessel was removed for analysis, and a new CO2 absorber was connected farest the carboy. Precipitation of BaCO3 in the second CO2 trap indicated that the absorber bottle nearest the test vessel had to be changed and analysed for CO2.
- Number of culture flasks/concentration: 2 (test item), 1 (Sodium Benzoate), 1 (toxicity control), 2 (blanks)
- Method used to create aerobic conditions: Before starting the test, the mineral nutrient solution with inoculum but without any test component were aerated for 24 hours with CO2-free compressed air (use of a 'CO2 scrubbing apparatus' described in the OECD Test Guideline 301 B), in order to purge the system of carbon dioxide.
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency: Titrations were performed at t3d, t6d, t10d, t14d, t17d, t22d, t28d, and t29d.
- Sampling method: CO2 generated by the test item was trapped by 0.025 N Ba(OH)2 in a trap system described in the Guideline. Resting Ba(OH)2 (0.025 N) in the CO2 trap removed from the trap system was titrated with HCl (0.05 N) using phenolphthalein as an indicator.
CONTROL AND BLANK SYSTEM
- Inoculum blank: For Blank correction two test solutions were prepared in the same way as the test solutions with the test item, but without the addition of any test- or control item.
- Toxicity control: One further test solution was prepared using 123.5 mg sodium benzoate and 68.1 mg of the test item for toxicity control.
- Other: At the same time one test solution containing 123.3 mg sodium benzoate/3.5 L (ca. 20 mg C/L) of test solution was run in parallel.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 17
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- mean of 23% and 11% degradation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The study was conducted under GLP according to OECD 301 B without deviations on the registered substance itself. The method is to be considered scientifically reasonable and suitable for the test item, the available information allows the conclusion that the test was properly conducted, the positive control showed the appropriate results. Hence, the results can be considered as reliable to assess ready biodegradability of Isobutyl 4-chloro-3,5-diaminobenzoate. The test item attained 17% biodegradation after 28 days and therefore cannot be considered as readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
- Executive summary:
Isobutyl 4-chloro-3,5-diaminobenzoate was tested for biodegradability according to 'CO2-Evolution-Test' (OECD Guideline 301B). Calculated from the organic carbon content of the test item and the measured CO2 generation, 23 % of the theoretical CO2 (ThCO2) has been generated by the test item within 28d of test period in the first culture with 67.4 mg of test item. 11% of the theoretical CO2 (ThCO2) has been generated by the test item within 28d in the second culture with 67.7 mg of test item. The calculated mean degradation value of the test item was 17%. Thus, the test item may be regarded as “not readily biodegradable". The control item sodium benzoate was degraded 85 %, and the conditions of the "10-days-window" were met within 6 days. The total CO2-evolution of the Blank was 115 mg CO2. Thus the test is regarded to be valid. The results obtained with the toxicity control indicate that there was no toxicity of the test item towards microorganisms at the concentration used within the test as a degradation value of >30% was achieved in total within that treatment.
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