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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable publication

Data source

Reference
Reference Type:
publication
Title:
The results of microbial mutation test for forty-three industrial chemicals
Author:
Shimizu et al..
Year:
1985
Bibliographic source:
Jpn. J. Ind. Health 27, 400-419

Materials and methods

Principles of method if other than guideline:
Method: other: similar to OECD Guideline 471
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethoxyethanol
EC Number:
203-804-1
EC Name:
2-ethoxyethanol
Cas Number:
110-80-5
Molecular formula:
C4H10O2
IUPAC Name:
2-ethoxyethanol
Details on test material:
purity in all test = 98 %
Name of test substance as stated in publication: 2-Ethoxyethanol

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
Metabolic activation:
with and without
Metabolic activation system:
S9 from liver of male Sprague-Dawley rats
Test concentrations with justification for top dose:
5, 10, 50, 10, 500, 1000, 5000 µg/plate
Controlsopen allclose all
Positive controls:
yes
Positive control substance:
benzo(a)pyrene
Positive controls:
yes
Positive control substance:
9-aminoacridine
Positive controls:
yes
Positive control substance:
N-ethyl-N-nitro-N-nitrosoguanidine
Positive controls:
yes
Positive control substance:
4-nitroquinoline-N-oxide
Positive controls:
yes
Positive control substance:
2-nitrofluorene
Positive controls:
yes
Positive control substance:
other: 2-aminoanthracene
Positive controls:
yes
Positive control substance:
furylfuramide
Details on test system and experimental conditions:
OTHER: Ames Test

Results and discussion

Test results
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks on result:
other: other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538; Escherichia coli WP2uvrA
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
According to the results in this gene mutation assay the test substance 2-ethoxyethanol was not mutagenic to the microbial strains of Salmonella typhimurium (TA 98, TA 100, TA 1535, TA 1537, TA 1538) and Escherichia coli (WP2uvrA).

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