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The inherent property of HDI trimer is to hydrolyse rapidly in an aquatic environment.

In the following the toxicity of the hydrolysis product(s) are reported for tests which have been conducted with specially layed down test conditions and which are thus reproducible tests:

For fish (Danio rerio) the acute toxicity expressed in terms of lethal loadings (LL0, 96 h) of HDI trimer was >= 100 mg/l. The studies were conducted according to Directive 92/69/EEC, C1 (Bayer AG, 2000/Bayer AG, 2001).

HDI trimer was tested with respect to the acute toxicity towards invertebrates (Daphnia magna) according to Directive 92/69/EEC, C2. After 48 hours effective concentration values expressed in terms of effective loadings (EL0, 48 h) of >= 100 mg/l and 25 mg/l, respectively were obtained. The 48 h-EL100 for HDI trimer was > 100 mg/l (Bayer AG, 2001).

The effect of HDI trimer towards the growth inhibition of algae (Desmodesmus subspicatus) was assessed performing a study according to the German standard method “DIN 38 412, Part 9 (1989) "Zellvermehrungshemmtest: Scenedesmus subspicatus CHODAT (Grünalge)", which is in most part equivalent to the OECD TG. In 72 hours the nominal concentration which reduces the growth rate by 10 % was 370 mg/L and the one which reduces by 50 % was > 1000 mg/L (Bayer AG, 1989).

In another test the toxicity of HDI trimer to algae (Desmodesmus subspicatus CHODAT) was assessed performing a study according to the Directive 92/69/EEC, C.3: “Algal inhibition test”, which is in most parts equivalent to the OECD TG 201. No inhibition of the algal population as reduction in growth rate was observed after 72 h at a test concentration of 100 mg/l (Bayer AG, 2001).