2-(2-methoxyethoxy)ethanol

Immunotoxicity

Administrative data

Endpoint:

immunotoxicity: acute oral

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No data on analytical purity of test substance otherwise sufficiently well reported to judge the results reliable with restrictions for use in risk assessment.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Comparative immunosuppression of various glycol ethers orally administered to Fischer 344 rats.

Author:

Smialowicz RJ, Williams WC, Riddle, MM et al

Year:

1992

Bibliographic source:

Fundam Appl Toxicol, 18(4), 621-7

Reference 2

Reference Type:

publication

Title:

The immunotoxicity of 2-methoxyethanol and its metabolites.

Author:

Smialowicz RJ

Year:

1996

Bibliographic source:

Occup Hyg, vol 2, p269-74

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Rats orally administered substance in two doses over a 2 day period then the humoral immune system functionality assessed with a T-dependent antigen response assay

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-methoxyethoxy)ethanol

EC Number:

203-906-6

EC Name:

2-(2-methoxyethoxy)ethanol

Cas Number:

111-77-3

Molecular formula:

C5H12O3

IUPAC Name:

2-(2-methoxyethoxy)ethanol

Details on test material:

-Source:Aldrich Chemical Company, Milwaukee, Mo
- Analytical purity:no data

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
-Source: Source: Charles River Laboratory, Raleigh, NC
-Age at study initiation: 8-10 weeks
-Housing: 3 per polycarbonate cage, heat treated pine shavings bedding.
-Diet: Purina Lab Chow, Purina Mills, ad libitum
-Water: ad libitum

ENVIRONMENTAL CONDITIONS
-Temperature: 22C
-Humidity: 50-60%
-Photoperiod: 12hrs dark/12hrs light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: distilled water

Details on exposure:

Preparation of dosing solution: no data

Analytical verification of doses or concentrations:

no

Details on analytical verification of doses or concentrations:

dosing volume 2.5ml/kg

Duration of treatment / exposure:

2 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
50, 100, 200, 400mg/kg per day
Basis:

No. of animals per sex per dose:

6

Control animals:

yes, concurrent vehicle

Examinations

Humoral immunity examinations:

Method: Rats were immunized with a single intravenous injection of trinitrophenyl-Lipopolysaccharide (TNP-LPS) 2-4hrs prior to substance exposure. 3 days after final exposure, a splenic antibody plaque forming assay (PFC) using SRBC was carried out using the method of Smialowicz (Toxicology 44, 271, 1987).
Dose groups: See above
Number of animals: See above.

Statistics:

Results expressed as mean and standard deviation. Data analysed by one way analysis of variance with post analysis using Dunnett’s multiple comparison t-test or the Student-Newman-Kuels multiple range test as required. Significance level set at p<0.05.

Results and discussion

Results of examinations

Clinical signs:

not examined

Mortality:

not examined

Body weight and weight changes:

not examined

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

not examined

Clinical biochemistry findings:

not examined

Urinalysis findings:

not examined

Gross pathological findings:

not examined

Details on results:

Humoral immunity examinations: When expressed as cells/spleen, the 400mg/kg dose group showed a siginficant reduction but this was not evident in the 800mg/kg dose group, therefore the change in the 400mg/kg group was not regarded as biologically significant. No significant effects were seen in any of the other parameters assessed.

Specific immunotoxic examinations

Cell viabilities:

not examined

Humoral immunity examinations:

no effects observed

Specific cell-mediated immunity:

not examined

Non-specific cell-mediated immunity:

not examined

Other functional activity assays:

not examined

Other findings:

not examined

Effect levels

Dose descriptor:

NOAEL

Effect level:

> 800 mg/kg bw/day

Sex:

male

Basis for effect level:

other: Humoral immunity markers

Applicant's summary and conclusion

Conclusions:

Substance has no significant effects on humoral immunity.

Executive summary:

Treatment of rats acutely with 2 -(2 -methoxyethoxy)ethanol causes no significant suppression of the antibody response to TNP-LPS at doses up to 800mg/kg.