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EC number: 206-354-4
CAS number: 330-54-1
- Mutagenicity in bacteria
reliable study, the mutagenic potential of Diuron was tested in
Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100
equivalent to OECD Guideline 471. Test substance concentrations of 0,
125, 250, 500, 1000 and 2000 µg/plate in DMSO were tested with and
without the addition of a rat liver homogenate metabolising system
(S9-mix).No increase in the frequency of revertant colonies compared to
concurrent negative controls were observed in all tested strains,
neither in the presence nor in the absence of metabolic activation.
Thus, Diuron did not induce gene mutations in the four tested Salmonella
strains under the given test conditions (Herbold, 1984). In general,
this negative result was confirmed in additional tests using the same
Salmonella strains as well as the tester starin WP2 uvrA (Becker, 2008,
In vitro cytogenicity in mammalian cells
reliable in vitro mammalian chromosome aberration test was performed
with Diuron in Chinese hamster ovary (CHO) according to OECD Guideline
473 (Shivaram, 1999). Diuron concentrations of 0, 90, 180 and 360 µg/mL
were used but no evidence for the induction of chromosome aberrations
was obtained in the presence and absence of metabolic activation while
the respective positive control substances produced a statistically
significant increase in aberrant metaphases under identical conditions.
- In vitro mutagenicity in mammalian cells
reliable in vitro Mammalian Cell Gene Mutation Test was performed with
Diuron in Chinese hamster ovary (CHO/ HPRT assay) according to OECD
Guideline 476 (Rickard, 1985). The cells were treated with the test
substance in duplicate, together with vehicle (DMSO) and positive
controls (ethylmethanesulphonate and 9, 10-dimethylbenz (a) anthracene).
The test concentration without metabolic activation was: 0, 0.010,
0.500, 1.000, 1.125 or 1.250 mM. The test concentration with metabolic
activation was: 0, 0.050, 1.000, 0.200, 0.500 or 0.750 mM.
positive control materials induced marked increases in the mutant
frequency indicating the satisfactory performance of the test and of the
activity of the metabolising system. Diuron did not induce a
dose-related or biologically significant increase in the mutation
frequency at the HPRT-locus with and without activation. However,
significant cytotoxicity was noted in the two trials with metabolic
activation at 0.5 and 0.75 mM.
- in vivo micronucleus assay
micronucleus test in mice was conducted according to OECD 474 and EEC
guidelines.After single i.p.
treatment with 700 mg/kg bw Diuron no mortalities were observed, but all
animals showed signs of toxicity.No
increase in micronucleated PCEs over solvent control was noted at any
sampling time point, so no indications of a clastogenic effect of Diuron
available data on genetic toxicity is conclusive but not sufficient for
classification according to directives DSD (67/548/EEC) or CLP
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