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EC number: 421-660-1 | CAS number: - Z-27
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 June 1995 to 29 September 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed to a guideline and used GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1050 (Algal Toxicity, Tiers I and II)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis(dimethyl-(2-hydroxyethyl)ammonium) 1,2-ethanediyl-bis(2-hexadecenylsuccinate)
- EC Number:
- 421-660-1
- EC Name:
- Bis(dimethyl-(2-hydroxyethyl)ammonium) 1,2-ethanediyl-bis(2-hexadecenylsuccinate)
- Molecular formula:
- Hill formula: C50 H96 N2 O10 CAS formula: C42 H74 O8. 2(C4 H11 N O)
- IUPAC Name:
- bis((2-hydroxyethyl)dimethylazanium) (4E)-3-{[2-({3-carboxylato-3-[(2E)-hexadec-2-en-2-yl]propanoyl}oxy)ethoxy]carbonyl}-4-methyloctadec-4-enoate
- Reference substance name:
- BIS(DIMETHYL-(2-HYDROXYETHYL)AMMONIUM) 1,2-ETHANEDIYL-BIS (2-HEXADECENYLSUCCINATE)
- IUPAC Name:
- BIS(DIMETHYL-(2-HYDROXYETHYL)AMMONIUM) 1,2-ETHANEDIYL-BIS (2-HEXADECENYLSUCCINATE)
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The concentration and stability of the test material in the test solutions were verified by chemical analysis.
- Sampling method: Water samples were taken from the control and 100 mg/L test group (replicates pooled) at 0 and 96 hours for quantitative analysis.
- Sample storage conditions before analysis: NDA
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: No auxillary solvent was used.
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test material was prepared by a direct dispersion in culture medium. An amount of test material (100 mg) was dissolved in culture medium and the volume adjusted to 500 mL to give a 200 mg/L stock solution. This stock solution was mixed with 500 mL of algal suspension to give the test concentration of 100 mg/L.
- Eluate: NDA
- Differential loading: NDA
- Controls: The control was maintained under identical conditions but not exposed to the test material.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/A
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): N/A
- Evidence of undissolved material (e.g. precipitate, surface film, etc): NDA
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Liquid cultures were obtained from the Culture Centre for Algae and Protozoa (CCAP), Institute of Freshwater Ecology, Cumbria.
- Age of inoculum (at test initiation): The culture conditions gave an algal suspension in log growth phase growth charactersied by an absorbance of 0.534 (at 665 nm). This suspension was diluted to an absorbance of 0.025 prior to use.
- Method of cultivation: NDA
ACCLIMATION
- Acclimation period: NDA
- Culturing media and conditions (same as test or not): The culture medium used for the studies was the same as that used to maintain the stock culture.
- Any deformed or abnormal cells observed: NDA
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- NDA
- Test temperature:
- 24 ± 1 °C
In the first 35 minutes of the study the temperature in the incubator dropped to 21.9-22.9 °C but this was caused by the incubator being opened to allow the test vessels to be placed in the incubator. - pH:
- The pH of the culture medium after equilibration with air was approximately 7.5.
Mean pH of control at 0h: 8.0
Mean pH of control at 96 h: 9.9
Mean pH of test sample flasks at 0 h: 8.1
Mean pH of test sample flasks at 96 h: 10.1 - Dissolved oxygen:
- NDA
- Salinity:
- NDA
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Mean measured concentration at 0 h: 80 mg/L
Mean measured concentration at 96 h: 63 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Closed - the flasks were covered with aluminium foil
- Material, size, headspace, fill volume: Six flasks each containing 100 mL of solution were prepared for the treatment group
- Aeration: The flasks were constantly shaken at 100 rpm for 96 hours
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- Initial cells density: 10^4 cells per mL
- Control end cells density: Mean cell density of control at 96 h: 2.24x10^6 cells/mL
- No. of organisms per vessel: N/A
- No. of vessels per concentration (replicates): 6 vessels
- No. of vessels per control (replicates): 3 vessels
- No. of vessels per vehicle control (replicates): N/A
GROWTH MEDIUM
- Standard medium used: Yes
- Detailed composition if non-standard medium was used: The composition of the culture medium was identical to that given in the OECD guideline 201 with the following exceptions:
Na2EDTA·2(H2O) was not present
FeCl3·6(H2O) was present at 0.08 mg/L instead of 0.064 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: NDA
- Total organic carbon: NDA
- Particulate matter: NDA
- Metals: NDA
- Pesticides: NDA
- Chlorine: NDA
- Alkalinity: NDA
- Ca/mg ratio: NDA
- Conductivity: NDA
- Culture medium different from test medium: No
- Intervals of water quality measurement: The pH of each control and test flask was determined at initiation of the study and after 96 h exposure.
OTHER TEST CONDITIONS
- Sterile test conditions: NDA
- Adjustment of pH: No
- Photoperiod: Continuous illumination
- Light intensity and quality: Intensity approximately 7000 lux
- Salinity (for marine algae): N/A
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Samples were taken at 0, 24, 48, 72 and 96 hours and the cell densities determined by direct counting with the aid of a haemocytometer.
- Chlorophyll measurement: None
- Other: None
TEST CONCENTRATIONS
- Spacing factor for test concentrations: NDA
- Justification for using less concentrations than requested by guideline: See below
- Range finding study
- Test concentrations: Nominal test concentrations: 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: The results showed no effect on growth at all concentrations tested. Based on the result of the range finding study a limit test was conducted for the definitive study at a test concentration of 100 mg/L to confirm that at the maximum test concentration given in the test guidelines, no effect on algal growth was observed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 63 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 63 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 63 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 63 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): The cell concentration of the control cultures increased by a facotr of 36 after 72 hours and by a factor of 182 after 96 h.
- Observation of abnormalities (for algal test): There were no abnormalities detected in any of the control or test cultures.
- Any stimulation of growth found in any treatment: Neither the growth (r) or the biomass (b) of Selenastrum capricornutum was affected by the presence of 100 mg/L of the test material over the 96 h exposure period.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: NDA
- Effect concentrations exceeding solubility of substance in test medium: NDA - Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- NDA
Any other information on results incl. tables
Table 1 Inhibition of growth
Nominal concentration (mg/L) |
Area under curve at 72 h |
% inhibition |
Area under curve at 96 h |
% inhibition |
Growth rate (0-24 h) |
% inhibition |
Control |
1.17x107 |
- |
4.36x107 |
- |
0.078 |
- |
100 |
1.39x107 |
[19] |
4.62x107 |
[6] |
0.085 |
[9] |
[increase in growth as compared to control]
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC values based on the 96 h mean measured test concentration were greater than 63 mg/L. Correspondingly the NOEC was greater than or equal to 63 mg/L.
- Executive summary:
In a 96 hour acute toxicity study, the cultures of the green alga Selenastratum capricornutum were exposed to OS 114451A at a nominal concentration of 100 mg a.i./L under static conditions in accordance with Method C.3 of Commission Directive 92/69/EEC. The NOEC and EC50values based on cell density were 100 and >100 mg a.i./L (nominal) and 63 and >63 mg a.i./L (measured concentration), respectively. There were no compound related phytotoxic effects.
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