Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to fish:

The 96 -hour LC50 value based on time-weighted-mean measured concentrations was 0.25 mg/L with 95% confidence limits of 0.22 - 0.28 mg/L. The NOEC was determined to be 0.18 mg/L.

Short-term toxicity to aquatic invertebrates

The 48-hour EC50 value based on time-weighted mean measured concentrations was greater than 1.2 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 1.2 mg/L.

 

Toxicity to aquatic algae

The 72-hour EC50 value based on the time-weighted mean measure test concentrations was greater than 1.5 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 1.5 mg/L.

Toxicity to microorganism

Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50 and EC80 were higher than 1000 mg/L. The NOEC was determined to be 1000 mg/L.

Additional information

Toxicity to fish:

TODI was tested for acute toxicity to fish according to EU-method C.1 and OECD guideline 203. The 96-hour LC50 based on nominal test concentrations was 1.0 mg/L with 95% confidence limits of 0.90 - 1.1 mg/L. The No Observed Effect Concentration was 0.72 mg/L.

TODI as such is hardly soluble in water (See IUCLID section 4.6., water solubility), therefore Dimethylformamide was used as auxiliary solvent / dispersant in the acute toxicity fish test. Moreover, TODI showed to be highly unstable in aqueous media, with hydrolysis occurring virtually instantaneously on contact with water and the degradation product was found to be insoluble in organic and aqueous solvents. (See IUCLID section 6.1.1, short-term toxicity to fish). That means that (1.) the acute toxicity test in fish represents the worst case as the solubility in water was increased by an auxiliary solvent and (2.) a chronic exposure to the TODI, respectively to its degradation product is negligible due to the insolubility of both if no auxiliary solvent is used. Since (2.) represents the real and not the worst case scenario, a long-term fish study is not justified.

Toxicity to daphnia:

The acute toxicity of the test material TODI to the freshwater invertebrate Daphnia magna has been investigated and based on the nominal concentration gave a 48-hour EC50 of greater than 4.0 mg/L. Correspondingly the No Observed Effect Concentration was greater than or equal to 4.0 mg/L. The 48-hour EC50 value based on time-weighted mean measured concentrations was greater than 1.2 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 1.2 mg/L.

TODI as such is hardly soluble in water (See IUCLID section 4.6., water solubility), therefore Dimethylformamide was used as auxiliary solvent / dispersant in the acute toxicity fish test. Moreover, TODI showed to be highly unstable in aqueous media, with hydrolysis occurring virtually instantaneously on contact with water and the degradation product was found to be insoluble in organic and aqueous solvents (see IUCLID section 6.1.3, short-term toxicity to aquatic invertebrate). That means that (1.) the acute toxicity test in aquatic invertebrate represents the worst case as the solubility in water was increased by an auxiliary solvent and (2.) a chronic exposure to the TODI, respectively to its degradation product is negligible due to the insolubility of both if no auxiliary solvent is used. Since (2.) represents the real and not the worst case scenario, a long-term study in aquatic invertebrate is not justified.

Toxicity to algae:

TODI was assessed in aquatic toxicity test to algae according EU-method C.3 and OECD guideline 201. The 72-hour EC50 value (nominal) was greater than 4.0 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 4.0 mg/L. The 72-hour EC50 value based on the time-weighted mean measure test concentrations was greater than 1.5 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 1.5 mg/L.

Toxicity to aquatic microorganisms:

TODI was assessed in an activated sludge respiration inhibition test, according to the EU-method C. 11 and OECD guideline 209. Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50 and EC80 were higher than 1000 mg/L. The NOEC was determined to be 1000 mg/L.