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EC number: 904-155-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 Dec 2020 - 25 Jan 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Guideline study to GLP standards with no deviations
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Commission Regulation (EC) No. 440/2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: A stock solution containing 100.4 mg/L test item in M4-medium was prepared. The lower treatments (4.6 / 10 / 22 / 46 mg/L nominal) were prepared by dilution of this stock solution with M4-medium.
- Sampling method: QC samples (11 mg/L)
- Sample storage conditions before analysis: closed vessel at room temperature (17.3 –23.0 °C). - Vehicle:
- no
- Details on test solutions:
- Before measurement, the taken samples of the concentrations 22 / 46 / 100 mg/L were diluted with demineralised water as follow: 500 μL of the sample with a concentration of 22 mg/L was diluted with 500 μL demineralised water (2-fold dilution). 250 μL of the sample with a concentration of 46 mg/L was diluted with 750 μL demineralised water (4-fold dilution). 500 μL of the sample with a concentration of 100 mg/L was diluted with 500 μL demineralised water (2-fold dilution). Afterwards, 250 μL of this solution was diluted with 750 μL of demineralised water (8-fold dilution at the end).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.
Adult female daphnids were maintained in glass vessels containing Elendt M4 medium in a temperature controlled room maintaining the water temperature at 18 to 22 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with neon LED's. Each culture was fed with a mixture of algal suspension (Desmodesmus subspicatus). Culture conditions ensured that reproduction was by parthenogenesis. 18.75 hours before the start of the test, the adult animals were separated from the young. 0.75 hours before the test start, adults were caught with the help of a glass tube. Newborn daphnia (age < 24h) were sieved from the medium and immediately placed into a beaker containing dilution water. After the acclimation period, animals which showed no apparent damage were used for the test. The diet and diluent water are considered not to contain a contaminant that would affect the integrity or outcome of the study. - Test type:
- flow-through
- Water media type:
- other: Reconstituted water (Elendt M4 medium)
- Remarks:
- The test item was melted in a water bath (max. 80°C) until it was liquid. A stock solution containing 100.4 mg/L test item in M4-medium was prepared. The lower treatments were prepared by dilution of this stock solution with M4-medium.
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Observations at 24 and 48h
- Hardness:
- 294 mg/L as CaCl2*2H2O
- Test temperature:
- 20.0 – 20.4 °C
- pH:
- 7.5-7.9
- Dissolved oxygen:
- 8.6-8.9 mg O2/L
- Salinity:
- Not applicable
- Conductivity:
- Not stated
- Nominal and measured concentrations:
- 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)
The concentrations tested are based on the result of a non-GLP pre-test. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50mL glass
- Volume of solution: 20 ± 5 mL
- No. of organisms per vessel: five daphnids
- No. of vessels per concentration/control (replicates): Four replicate test and control vessels
TEST MEDIUM / WATER PARAMETERS
CaCl2.2H2O 293.8 mg/L
MgSO4.7 H2O 123.3 mg/L
NaHCO3 64.8 mg/L
KCl 5.8 mg/L
Na2SiO3.9 H2O 10.0 mg/L
NaNO3 0.274 mg/L
K2HPO4 0.184 mg/L
KH2PO4 0.143 mg/L
H3BO3 2.8595 mg/L
Na2EDTA.2 H2O 2.50 mg/L
FeSO4.7 H2O 0.9955 mg/L
MnCl2.4H2O 0.3605 mg/L
LiCl 0.306 mg/L
SrCl2.6 H2O 0.152 mg/L
RbCl 0.071 mg/L
Na2MoO4.2 H2O 0.0615 mg/L
CuCl2.2 H2O 0.0165 mg/L
NaBr 0.0160 mg/L
ZnCl2 0.0130 mg/L
CoCl2.6 H2O 0.010 mg/L
KI 0.00325 mg/L
Na2SeO3 0.00219 mg/L
NH4VO3 0.000575 mg/L
Thiamine hydrochloride 0.075 g/L
Cyanocobalamine 0.0010 g/L
D(+) biotin (Vitamin H) 0.00075 g/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- None of the animals were immobilised in the blank control. The highest tested concentration showed 5 % immobilisation, but this could be stated as not significant. Only one daphnia died in the highest tested concentration of 100 mg/L and it is not clear if this is a reaction on the test substance or only a biological reason. Therefore, 5 % immobilisation is considered not significant.
No sub-lethal effects of exposure were observed throughout the test in the test groups, however, one daphnid from the control and solvent control was observed to be coated in algae at 48 hours.
The test was considered to be valid given that less than 10% of the control and solvent control daphnids showed immobilization or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L
Temperature was maintained at approximately 19C to 20C throughout the test, while there were no treatment related differences for oxygen concentration or pH in the control and test vessels
At the start and throughout the test all control and test solutions were observed to be clear colorless solutions.
- Behavioural abnormalities: None stated
- Observations on body length and weight: None stated
- Other biological observations: None stated
- Mortality of control: No
- Other adverse effects control: one daphnid from the control and solvent control was observed to be coated in algae at 48 hours
- Immobilisation of control:
- Abnormal responses: None stated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None stated
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- The results from the positive control with potassium dichromate were within the normal range ( 0.6 mg/L to 2.1 mg/L) for this reference item
- Reported statistics and error estimates:
- All validity criteria were fulfilled:
- Immobilisation in the controls may not exceed 10 %. Immobilisation in the control was 0 %.
- The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.6 mg/L.
Because of no significant toxicity, no statistically evaluation was performed. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 value for HB TMP is above 100 mg/l (high concentration tested).
- Executive summary:
The 48 hr-acute toxicity of HB TMP to Daphnia magna flow through conditions. Test species were exposed to potassium dichromate and the test chemical at nominal concentration of 0,4, 6, 10, 22, 46, and 100 mg/L for 48 hr. Immobilisation and sublethal effects were observed daily. The 48 hour EC50 was greater than 100 mg/l. Based on the results of this study, HB TMP would not be classified as toxic to Daphnia magna to in accordance with the CLP classification. This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1st instar): Daphnia magna
Test Type (Flowthrough, Static, Static Renewal): Flowthrough
EC50: > 100 mg/L HB TMP
Endpoint(s) Effected: Immobilisation
Reference
Description of key information
In a 48 hr GLP acute toxicity study, Daphnia magna showed to have an EC50 value greater than 100 mg HB TMP/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- > 100 mg/L
Additional information
The 48 hr-acute toxicity of HB TMP to Daphnia magna flow through conditions. Test species were exposed to potassium dichromate and the test chemical at nominal concentration of 0, 4, 6, 10, 22, 46, and 100 mg/L for 48 hr. Immobilisation and sublethal effects were observed daily. The 48 hour EC50 was greater than 100 mg/l. Based on the results of this study, HB TMP would not be classified as toxic to Daphnia magna to in accordance with the CLP classification
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