1-methoxy-2-propanamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-03-06 to 1989-03-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

609-333-1

EC Number:

609-333-1

Cas Number:

37143-54-7

Molecular formula:

C4 H11 N O

IUPAC Name:

609-333-1

Details on test material:

- Analytical purity: 99.2%
- Test substance No.: 88/512
- Physical state: Liquid
- Homogeneity: Ensured since the substance is a genuine liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An aqueous solution was prepared (5 g/500 mL resp. 2.5 g/250 mL); subsequently the fish were placed into the aquaria
- Controls: Reconstitued testwater according to guideline; preparation from fully demineralized tap water

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Age of test organisms: >= 1 month
- Length at study initiation: 6 cm (range: 5.5 - 7.1)
- Weight at study initiation: 1.8 g (range: 1.2 - 2.8)

HOUSING
- Type and amount of food: Growing Feed F/B 50, ad libitum
- Mortality during the time of housing: about 0.4 - 2.4%
- Mortality during the adaptation period: 0%

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

About 2.5 mmol/L

Test temperature:

20 - 21 °C

pH:

7.4 - 7.7 (pH adjusted in the concentration 215 mg/L)
9.8 in the test concentration 215 mg/L without adjusted pH

Dissolved oxygen:

6.9 - 8.6 mg/L

Nominal and measured concentrations:

Nominal: 0, 10, 21.5, 46.4, 100.0 and 215.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30x22x24 cm)
- Fill volume: 10 liters
- Aeration: continuous aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.8 g/L
- Corpulence factor of the batch: 0.8 (determined on 1989-03-03)
- Withdrawal of food: One day before and during exposure

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Conductivity: max. 10 µMHO
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness

EFFECT PARAMETERS MEASURED: Mortality, symptoms

TEST CONCENTRATIONS
- Range finding study: Yes
- Test concentrations: 0, 10, 21.5, 46.4, 100.0 and 215.0 mg/L (nominal values)
- Results used to determine the conditions for the definitive study: Yes, LC50 was determiend to be between 10 and 100 mg/L
- To study the effects of the hgh pH on the toxicity, the concentration 215 mg/L was added 2 weeks later after pH-adjustment using hydrochloric acid.

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

symptoms

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 46 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Symptoms observable: None
- Mortality of control: None
- Any observations that might cause a difference between measured and nominal values: None

Results with reference substance (positive control):

- Mortality with reference substance: LC50 after 48 hours: About 23 mg/L (determined on 1989-03-06)

Reported statistics and error estimates:

Statistics used: Probit analysis