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EC number: 611-056-6 | CAS number: 538313-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2011
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: other: GLP certificate is not present in the report (Reliability Guarantee present). IUCLID informations according to english translation of the Japanese report ( CERI: 2011-03-29)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Remarks:
- 30 mg/L (as a suspended substance concentration). The suspended substance concentration of the active sludge was 3400 mg/L.
- Details on inoculum:
- Sludge (surface water of rivers, lakes, and sea inlets including topsoil, and sewage treatment facility return sludge) was collected from 10 locations in Japan according to the test method of 5 a), and active sludge (collection period: December 2010, Usage Start: January 5, 2011) that had been prepared and managed at this test facility were used. Synthesized sewage (glucose, peptone, di-hydrogen potassium phosphate were dissolved in purified water, and adjusted to a pH or 7.0±1.0), and the activated sludge used after 22 hours.
- Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 100 mg/L
- Based on:
- test mat.
- Remarks:
- 30 mg of the test sample were directly incorporated into the test flask (300 mL)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- Detection of 5,5'-bi-1H-tetrazole; copper (water soluble and insoluble) and ammonia
- Details on study design:
- TEST CONDITIONS
Supportive Medium 5L were prepared at a proportion where 3 mL of the respective A, B, C, and D solutions (according to JIS K 0102-2010) were added to 1L of purified water
pH adjusted to 7.0
Tested Substance Concentration: 100 mg/L
Active Sludge Concentration: 30 mg/L (As a suspended substance concentration)
Test Fluid Volume: 300 mL
Test Fluid Culture Temperature: 25±1°C
Test Fluid Culture
Time: 28 days (light-shielded)
Stirring method: rotating agitation with a stirrer
TEST SYSTEM
Closed-system Oxygen Demand Equipment
Thermostatic Chamber Data Processing Equipment
Test FluidAbiotic control to 100 mg/l
(Water + Tested Material): 1 Test Container [1]
300 mL of purified water and 30 mg of the tested substance were entered into a test container. Test suspension to 100 mg/L
(Sludge + Tested Material): 3 Test Containers [2][3][4]
The supportive medium [amount of the added active sludge volume (2.65 mL) subtracted from 300 mL] as well as the test sample 30 mg were entered into the test container. Procedure control to 100 mg/L
(Sludge + Aniline): 1 Test Container [6]
The supporting medium [amount with the added active sludge volume (2.65 mL) subtracted from 300 mL] and aniline 29.5 µL (30 mg) were entered into the test container so that the aniline concentration was 100 mg/L. The aniline was added after fractionation with a micro syringe.
Sludge Blank: 1 Test Container [5]
The supporting medium [amount with the added active sludge volume (2.65 mL) subtracted from 300 mL] and was entered into the test container - Reference substance:
- aniline
- Remarks:
- 100 mg/L (CAS number: 62-53-3; supplier: Wako Pure Chemical Industries; lot number: EPL1617)
- Preliminary study:
- From the results of the preliminary studies, it was determined that the solubility of the test material was very low in general-purpose solvents, and that 5,5'-bi-1H-tetrazole, copper and ammonia were liberated from the tested material in the test solution, and therefore it would be difficult to apply it to analysis in the tested product state. Thus, after the completion of the culturing period, a quantitative analysis was performed on the 5,5'-bi-1H-tetrazole, copper, and ammonia constituting the tested substance in order to estimate the biodegradation of the test material.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 3
- Sampling time:
- 28 d
- Remarks on result:
- other: continuous measurements
- Key result
- Parameter:
- other: Detection rate of 5,5'-bi-1H-tetrazole
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Detection rate for water-soluble 5,5'-bi-1H-tetrazole: 59% and for water insoluble 5,5'-bi-1H- tetrazole: 40%.
- Key result
- Parameter:
- other: Detection rate of copper
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Detection rate of water soluble copper: 0% and water insoluble copper: 97.7%
- Key result
- Parameter:
- other: Detection rate of ammonia
- Value:
- ca. 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Detection rate of ammonia: 67.7%
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Remarks on result:
- other: Quantitative analysis of the test material can not be performed, the degradation by direct analysis of the tested material was not calculated.
- Details on results:
- Average percentage detection of DOC: 42% (sludge + test material); 66% (water + test material)
These results implied that part of the 5,5'-bi-1H-tetrazole was not liberated from the tested material.
Average percentage detection of water-soluble 5,5'-bi-1H-tetrazole: 59% (sludge + test material); 64% (water + test material)
Average percentage detection of water insoluble 5,5'-bi-1H-tetrazole: 40% (sludge + test material); 36% (water + test material)
Thereby confirming that structural changes did not occur in the 5,5'-bi-1H-tetrazole after 28 days of incubation.
Average percentage detection of water-soluble copper: 0% (sludge + test material); 6% (water + test material)
Average percentage detection of water insoluble copper: 97.7% (sludge + test material); 97% (water + test material)
All copper constituting the tested material was released into the water and resides in an insoluble inorganic state.
Average percentage detection of ammonia: 67.7% (sludge + test material); 95% (water + test material). - Results with reference substance:
- % biodegradation BOD 28 d: 72%% biodegradation DOC 28 d: Not available
- Validity criteria fulfilled:
- yes
- Remarks:
- Difference between extremes of replicates values of % biodegradation (by BOD): 6% (< 20%); % biodegradation of aniline by BOD: after 7 days 61% (> 40%) and after 14 days 71% (> 65%); BOD value of control blank (after 28 days): 6.7 mg (< 60 mg/L)
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Report conclusion: Under these test conditions, 5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced.In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.
- Executive summary:
5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced. In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.
Under test condition no biodegradation is observed.
Reference
Percentage biodegradation of reference and test material (O2 consumption):
Vessel | 7th day | 7th day | 14th day | 14th day | 21st day | 21st day | 28th day | 28th day | Mean |
No. | BOD (mg) |
Deg. (%) |
BOD (mg) |
Deg. (%) |
BOD (mg) |
Deg. (%) |
BOD (mg) |
Deg. (%) |
Deg. (%) |
[6] |
57.2 |
61 |
67.9 |
71 |
71.6 |
72 |
71.6 |
72 |
|
[5] |
2.0 |
- |
4.0 |
- |
6.7 |
- |
6.7 |
- |
|
[2] |
1.7 |
-1 |
4.5 |
1 |
9.0 |
4 |
9.0 |
4 |
3 |
[3] |
1.6 |
-1 |
5.4 |
2 |
9.3 |
5 |
9.3 |
5 |
|
[4] |
1.5 |
-1 |
3.1 |
-2 |
6.0 |
-1 |
6.2 |
-1 |
|
[1] |
0.0 |
- |
0.3 |
- |
1.0 |
- |
1.0 |
- |
|
[1]: water + test material
[2] [3] [4]: sludge + test material
[5]: control blank
[6]: sludge + aniline
Description of key information
5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced. In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.
Under test condition no biodegradation is observed.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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