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EC number: 619-290-0 | CAS number: 97780-06-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 002
- Cas Number:
- 97780-06-8
- Test material form:
- solid
- Details on test material:
- Purity: 98.9%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Community Sewerage Plant, Bharath Electronics Limted, Vidyaranyapuram, Bangalore, INDIA.
- Preparation of inoculum for exposure: This effluent was allowed to settle for one hour and decanted. The decanted effluent was then used in the test
- Bacterial population: The bacterial population in the inoculum was determined as colony forming units (CFU/mL) by diluting the inoculum up to 10e-4 dilution and then plating on nutrient agar plates.
- Preconditioning: The decanted effluent was preconditioned by aerating for 6 days at 21.3 to 23.3°C. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 33.33 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: A - Potassium dihydrogen orthophosphate, Dipotassium hydrogen orthophosphate, Disodium hydrogen orthophosphate dihydrate, Ammonium chloride (These four constituents were dissolved in and made up to 1000 mL using Milli-Q water. The pH of the solution was 7.53); B - Calcium chloride dihydrate (This was dissolved in and made up to 1000 mL using Milli-Q water); C - Magnesium sulphate heptahydrate (This was dissolved in and made up to 1000 mL using Milli-Q water); D - Iron (III) chloride hexhydrate (This was dissolved in and made up to 1000 mL using Milli-Q water).
The test medium for test flasks, was prepared by mixing 24 mL of solution A with 1920 mL of Milli-Q water, then 2.4 mL each of solution B, C and D was added and finally the volume was made up to 2400 mL with Milli-Q water in each flask. Similarly, 3000 mL of test medium was prepared by mixing 30 mL of solution A with 2400 mL of Milli-Q water, then 3 mL each of solution B, C and D was added and finally the volume was made up to 3000 mL with Milli-Q water.
- Test temperature: 20.9-23.0°C
- pH: 7.53 to 7.58
TEST SYSTEM
- Culturing apparatus: 5-L flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The flasks were aerated with CO2-free air at 43 to 50 mL/minute, overnight to purge the system of carbon dioxide
- Measuring equipment: Bubble flow meter
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2400 mL mineral salts medium, 300 mL additional mineral salts medium and 300 mL inoculum
- Procedure control: 2400 mL mineral salts medium, 300 mL reference standard solution (75.2 mg reference standard in 300 mL mineral salts medium) and 300 mL inoculum
- Toxicity control: 2400 mL mineral salts medium, 300 mL inhibition suspension (50 mg test item and 37.6 mg reference standard in 300 mL mineral salts medium) and 300 mL inoculum
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- Cumulative CO2 production in the controls was 106.61 mg CO2/3L that was typical for this type of test and inoculum source and was within the acceptable range for this assay system.
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 12.67
- Sampling time:
- 6 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 18.62
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 30.74
- Sampling time:
- 29 d
- Details on results:
- The degradation of sodium benzoate was also rapid in the presence of test substance and had achieved 63.23% of its CO2 after 14 days. These results show that the test substance did not cause any inhibitory effect on the test system at this concentration.
BOD5 / COD results
- Results with reference substance:
- The degradation of sodium benzoate was rapid and had achieved 18.87% of its CO2 after 2 days, 38.52% after 4 days, 79.43% after 14 days and 99.49% after 29 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Cumulative CO2 production by the mixtures containing test substance had achieved 12.67% of ThCO2 after 6 days, 18.62% after 14 days and 30.74% after 29 days.
- Executive summary:
The present study was conducted to determine the ready biodegradability of the test substance. The ready biodegradability was tested using the CO2 Evolution Test (Modified Sturm Test) as per the OECD Guideline No. 301B.
The test substance was added to two test vessels at the concentration of 33.33 mg/L of mineral medium [equivalent to 14.62 mg Carbon (C)/L]. Two control treatments containing only the inoculum, one positive control treatment containing inoculum plus reference standard (sodium benzoate), and one toxicity control treatment containing the inoculum plus test substance and reference standard were also tested. All the treatments were prepared with inoculum from a secondary effluent treatment plant receiving predominantly domestic sewage.
Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released by each treatment mixture was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each test vessel. The residual barium hydroxide was measured on the 2, 4, 7, 10, 14, 18, 22, 25 and 29 days after the initiation of the test by titration. The pH of the treatment mixtures was measured on Day 28.
Sodium benzoate had biodegraded by 38.52% at Day 4 and 79.43% by Day 14 in the absence of the test substance meeting the validity criteria of the test. Test mixture containing sodium benzoate and the test substance had biodegraded by 63.23% at Day 14 showing that the test substance was not inhibitory at this concentration.
The cumulative CO2 production by mixtures containing only the test substance had achieved 30.74% degradation of test substance during the treatment period. the test substance had biodegraded by 1.33% by Day 2 and by 18.62% at Day 14. Based on the pass levels (60% bio-degradation in the 10-day window period), the test substance cannot be considered as readily biodegradable since only 30.74% degradation was achieved during the test period of 28 days. However, it may be considered biodegradable as there was 30.74% degradation during the treatment period.
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